Abstract
DNA synthesis of normal rat spleen cells in response to endotoxin increases markedly if adherent cells are first removed from the cell suspension. Addition of small numbers of purified macrophages to the cultures restores the response to a low level. T-deprived cells show these effects in very much lesser degree. Large numbers of macrophages completely suppress the response of both normal and T-deprived spleen. We conclude that two mechanisms of suppression are at work: a direct effect of macrophages and a macrophage-dependent "suppressor T cell" effect.
Full Text
The Full Text of this article is available as a PDF (342.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alter B. J., Bach F. H. Lymphocyte reactivity in vitro. I. Cellular reconstitution of purified lymphocyte response. Cell Immunol. 1970 Jul;1(2):207–218. doi: 10.1016/0008-8749(70)90008-0. [DOI] [PubMed] [Google Scholar]
- Britton S. When allogeneic mouse spleen cells are mixed in vitro the T-cells secrete a product which guides the maturation of B-cells. Scand J Immunol. 1972;1(1):89–98. doi: 10.1111/j.1365-3083.1972.tb03738.x. [DOI] [PubMed] [Google Scholar]
- Gershon R. K., Kondo K. Antigenic competition between heterologous erythrocytes. I. Thymic dependency. J Immunol. 1971 Jun;106(6):1524–1531. [PubMed] [Google Scholar]
- Gery I., Gershon R. K., Waksman B. H. Potentiation of the T-lymphocyte response to mitogens. I. The responding cell. J Exp Med. 1972 Jul 1;136(1):128–142. doi: 10.1084/jem.136.1.128. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gery I., Waksman B. H. Potentiation of the T-lymphocyte response to mitogens. II. The cellular source of potentiating mediator(s). J Exp Med. 1972 Jul 1;136(1):143–155. doi: 10.1084/jem.136.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirano S., Uyeki E. M. A study of mixed cell interactions between sensitized and allogeneic mouse spleen cells in vitro using the hemolytic plaque assay. J Immunol. 1971 Mar;106(3):619–628. [PubMed] [Google Scholar]
- Hoffmann M., Dutton R. W. Immune response restoration with macrophage culture supernatants. Science. 1971 Jun 4;172(3987):1047–1048. doi: 10.1126/science.172.3987.1047. [DOI] [PubMed] [Google Scholar]
- Holland P., Holland N. H., Cohn Z. A. The selective inhibition of macrophage phagocytic receptors by anti-membrane antibodies. J Exp Med. 1972 Mar 1;135(3):458–475. doi: 10.1084/jem.135.3.458. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mooney J. J., Waksman B. H. Activation of normal rabbit macrophage monolayers by supernatants of antigen-stimulated lymphocytes. J Immunol. 1970 Nov;105(5):1138–1145. [PubMed] [Google Scholar]
- Möller G., Sjöberg O. Effect of antigenic competition on antigen-sensitive cells and on adoptively transferred immunocompetent cells. Cell Immunol. 1970 May;1(1):110–121. doi: 10.1016/0008-8749(70)90064-x. [DOI] [PubMed] [Google Scholar]
- Nathan C. F., Karnovsky M. L., David J. R. Alterations of macrophage functions by mediators from lymphocytes. J Exp Med. 1971 Jun 1;133(6):1356–1376. doi: 10.1084/jem.133.6.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]