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. 1972 Oct 1;136(4):697–714. doi: 10.1084/jem.136.4.697

BLOOD MICROCIRCULATION IN THE LYMPH NODE DURING THE PRIMARY IMMUNE RESPONSE

Peter G Herman 1, Itaru Yamamoto 1, Harry Z Mellins 1
PMCID: PMC2139287  PMID: 5056671

Abstract

Microangiography performed after total blood replacement with contrast material provided complete visualization of the vascular structures of the lymph node. Starting of the 2nd day, there is capillary redistribution throughout the cortex of the lymph node. The previously rather avascular nodules dissolve, and the cortical lymphoid tissue becomes uniformly vascular. Beginning on the 2nd day and reaching its peak on the 5th day, there is a significant increase in diameter and density of the subcapsular and medullary cord capillaries. 15 days after the antigenic stimulus, the appearance of the microvasculature returns to normal. The postcapillary venules (the microvascular structures which follow the capillaries) are widely distributed throughout. Histologically, only a fraction of these venules have a high endothelial lining (HE venules). Therefore, it is suggested that among the postcapillary venules, those with high endothelial lining should be specifically denoted. Great individual variation in the number of HE venules was observed, and no correlation with the timing of the immune response could be established. Whether the microvascular changes described lead to cellular change or are mere expressions of it cannot definitely be stated. However, the significant hypervascularity along the intranodal lymph pathways and the diffuse, even redistribution of the capillaries and postcapillary structures could greatly facilitate the humoral and cellular exchange between the circulating blood, the circulating lymph, and the tissues of the lymph node.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURWELL R. G. Studies of the primary and the secondary immune responses of lymph nodes draining homografts of fresh cancellous bone (with particular reference to mechanisms of lymph node reactivity). Ann N Y Acad Sci. 1962 Oct 24;99:821–860. doi: 10.1111/j.1749-6632.1962.tb45365.x. [DOI] [PubMed] [Google Scholar]
  2. EHRICH W. E., DRABKIN D. L., FORMAN C. Nucleic acids and the production of antibody by plasma cells. J Exp Med. 1949 Aug 1;90(2):157–168. doi: 10.1084/jem.90.2.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. EVERETT N. B., CAFFREY R. W., RIEKE W. O. RECIRCULATION OF LYMPHOCYTES. Ann N Y Acad Sci. 1964 Feb 28;113:887–897. doi: 10.1111/j.1749-6632.1964.tb40710.x. [DOI] [PubMed] [Google Scholar]
  4. Ehrich W. STUDIES OF THE LYMPHATIC TISSUE : III. EXPERIMENTAL STUDIES OF THE RELATION OF THE LYMPHATIC TISSUE TO THE NUMBER OF LYMPHOCYTES IN THE BLOOD IN SUBCUTANEOUS INFECTION WITH STAPHYLOCOCCI. J Exp Med. 1929 Feb 28;49(3):347–360. doi: 10.1084/jem.49.3.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ford W. L., Gowans J. L. The role of lymphocytes in antibody formation. II. The influence of lymphocyte migration on the initiation of antibody formation in the isolated, perfused spleen. Proc R Soc Lond B Biol Sci. 1967 Sep 12;168(1012):244–262. doi: 10.1098/rspb.1967.0064. [DOI] [PubMed] [Google Scholar]
  6. Fukuda J. Studies on the vascular architecture and the fluid exchange in the rabbit popliteal lymph node. Keio J Med. 1968 Mar;17(1):53–70. doi: 10.2302/kjm.17.53. [DOI] [PubMed] [Google Scholar]
  7. GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
  8. GOWANS J. L. The effect of the continuous re-infusion of lymph and lymphocytes on the output of lymphocytes from the thoracic duct of unanaesthetized rats. Br J Exp Pathol. 1957 Feb;38(1):67–78. [PMC free article] [PubMed] [Google Scholar]
  9. HALL J. G., MORRIS B. The lymph-borne cells of the immune response. Q J Exp Physiol Cogn Med Sci. 1963 Jul;48:235–247. doi: 10.1113/expphysiol.1963.sp001660. [DOI] [PubMed] [Google Scholar]
  10. HARRIS T. N., HARRIS S. Histochemical changes in lymphocytes during the production of antibodies in lymph nodes of rabbits. J Exp Med. 1949 Aug 1;90(2):169–180. doi: 10.1084/jem.90.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hall J. G., Morris B., Moreno G. D., Bessis M. C. The ultrastructure and function of the cells in lymph following antigenic stimulation. J Exp Med. 1967 Jan 1;125(1):91–110. doi: 10.1084/jem.125.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Herman P. G., Ohba S., Mellins H. Z. Blood microcirculation in the lymph node. Radiology. 1969 Apr;92(5):1073–1080. doi: 10.1148/92.5.1073. [DOI] [PubMed] [Google Scholar]
  13. LEDUC E. H., COONS A. H., CONNOLLY J. M. Studies on antibody production. II. The primary and secondary responses in the popliteal lymph node of the rabbit. J Exp Med. 1955 Jul 1;102(1):61–72. doi: 10.1084/jem.102.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MARSHALL A. H. E., WHITE R. G. Reactions of the reticular tissues to antigens. Br J Exp Pathol. 1950 Apr;31(2):157–174. [PMC free article] [PubMed] [Google Scholar]
  15. McGregor D. D., Gowans J. L. THE ANTIBODY RESPONSE OF RATS DEPLETED OF LYMPHOCYTES BY CHRONIC DRAINAGE FROM THE THORACIC DUCT. J Exp Med. 1963 Jan 31;117(2):303–320. doi: 10.1084/jem.117.2.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. RINGERTZ N., ADAMSON C. A. The lymph-node response to various antigens; an experimental-morphological study. Acta Pathol Microbiol Scand Suppl. 1950;86:1–69. [PubMed] [Google Scholar]

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