Abstract
The mechanism by which Bordetella pertussis organisms and their products induce lymphocytosis in mice was analyzed in terms of the localization of syngeneic Cr-51-labeled lymph node cells. Labeled lymphoid cells incubated in vitro with the supernatant of B. pertussis cultures and then injected intravenously into normal recipients, or labeled cells injected into pertussis-treated recipients were unable to "home" to lymphoid organs but persisted for long periods in the blood. In animals "equipped" with a population of Cr-51-labeled lymphoid cells, administration of B. pertussis organisms or culture supernatant effected a shift of radioactivity from lymph nodes and spleen into the peripheral blood, coincident with the lymphocytosis. In in vitro experiments it was found that the active principle could bind to both erythrocytes and lymphocytes and could spontaneously elute from these cells onto labeled lymphocytes which were then unable to home efficiently. The data suggest that Bordetella pertussis-induced lymphocytosis involves a reversible attachment of the pertussis factor onto the surfaces of lymphocytes which prevents their recirculation to lymphoid organs. Recirculating lymphocytes are presumably affected as they emerge from lymphoid organs to enter the blood.
Full Text
The Full Text of this article is available as a PDF (620.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dresser D. W., Taub R. N., Krantz A. R. The effect of localized injection of adjuvant material on the draining lymph node. II. Circulating lymphocytes. Immunology. 1970 May;18(5):663–670. [PMC free article] [PubMed] [Google Scholar]
- FARTHING J. R. The role of Bordetella pertussis as an adjuvant to antibody production. Br J Exp Pathol. 1961 Dec;42:614–622. [PMC free article] [PubMed] [Google Scholar]
- GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
- Lance E. M., Taub R. N. Segregation of lymphocyte populations through differential migration. Nature. 1969 Mar 1;221(5183):841–843. doi: 10.1038/221841a0. [DOI] [PubMed] [Google Scholar]
- Morse S. I., Barron B. A. Studies on the leukocytosis and lymphocytosis induced by Bordetella pertussis. 3. The distribution of transfused lymphocytes in pertussis-treated and normal mice. J Exp Med. 1970 Oct 1;132(4):663–672. doi: 10.1084/jem.132.4.663. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morse S. I., Bray K. K. The occurrence and properties of leukocytosis and lymphocytosis-stimulating material in the supernatant fluids of Bordetella pertussis cultures. J Exp Med. 1969 Mar 1;129(3):523–550. doi: 10.1084/jem.129.3.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morse S. I., Riester S. K. Studies on the leukocytosis and lymphocytosis induced by Bordetella pertussis. I. Radioautographic analysis of the circulating cells in mice undergoing pertussis-induced hyperleukocytosis. J Exp Med. 1967 Mar 1;125(3):401–408. doi: 10.1084/jem.125.3.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morse S. I., Riester S. K. Studies on the leukocytosis and lymphocytosis induced by Bordetella pertussis. II. The effect of pertussis vaccine on the thoracic duct lymph and lymphocytes of mice. J Exp Med. 1967 Apr 1;125(4):619–628. doi: 10.1084/jem.125.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pieroni R. E., Levine L. Adjuvant principle of pertussis vaccine in the mouse. Nature. 1966 Sep 24;211(5056):1419–1420. doi: 10.1038/2111419a0. [DOI] [PubMed] [Google Scholar]
- ROWLANDS D. T., Jr, CLAMAN H. N., KIND P. D. THE EFFECT OF ENDOTOXIN ON THE THYMUS OF YOUNG MICE. Am J Pathol. 1965 Feb;46:165–176. [PMC free article] [PubMed] [Google Scholar]
- Taub R. N., Gershon R. K. The effect of localized injection of adjuvant material on the draining lymph node. 3. Thymus dependence. J Immunol. 1972 Feb;108(2):377–386. [PubMed] [Google Scholar]
- Taub R. N., Krantz A. R., Dresser D. W. The effect of localized injection of adjuvant material on the draining lymph node. I. Histology. Immunology. 1970 Feb;18(2):171–186. [PMC free article] [PubMed] [Google Scholar]
- Taub R. N., Lance E. M. Histopathological effects in mice of heterologous antilymphocyte serum. J Exp Med. 1968 Dec 1;128(6):1281–1307. doi: 10.1084/jem.128.6.1281. [DOI] [PMC free article] [PubMed] [Google Scholar]