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. 1973 Jun 1;137(6):1311–1324. doi: 10.1084/jem.137.6.1311

ACTIVE SUPPRESSION OF IMMUNOGLOBULIN ALLOTYPE SYNTHESIS

III. IDENTITICATION OF T CELLS AS RESPONSIBLE FOR SUPPRESSION BY CELLS FROM SPLEEN, THYMUS, LYMPH NODE, AND BONE MARROW

Leonore A Herzenberg 1, Eva L Chan 1, Myrnice M Ravitch 1, Roy J Riblet 1, Leonard A Herzenberg 1
PMCID: PMC2139347  PMID: 4541122

Abstract

Thymus-derived cells (T cells) that actively suppress production of IgG2a immunoglobulins carrying the Ig-1b allotype have been found in adult (SJL x BALB/c)F1 mice exposed to anti-Ig-1b early in life. The suppression is specific for Ig-1b. The allelic product, Ig-1a, is unaffected. Spleen, lymph node, bone marrow, or thymus cells from suppressed mice suppress production of Ig-1b by syngeneic spleen cells from normal F1 mice. When a mixture of suppressed and normal cells is transferred into lethally irradiated BALB/c mice, there is a short burst of Ig-1b production after which Ig-1b levels in the recipient fall rapidly below detectability. Pretreatment of the cells from the suppressed mice with antiserum specific for T cells (anti-Thy-1b) plus complement before mixture destroys the suppressing activity. Similar results with suppressor cells were obtained in vitro using Mishell-Dutton cultures. Mixture of spleen cells from suppressed animals with sheep erythrocyte (SRBC)-primed syngeneic normal spleen before culture suppresses Ig-1b plaque-forming cell (PFC) formation while leaving Ig-1a PFC unaffected. Treatment of the suppressed spleen with anti-Thy-1b before transfer removes the suppressing activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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