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The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1973 Jun 1;137(6):1511–1525. doi: 10.1084/jem.137.6.1511

STUDIES ON CELL-MEDIATED IMMUNITY TO LYMPHOCYTIC CHORIOMENINGITIS VIRUS IN MICE

Ole Marker 1, Mogens Volkert 1
PMCID: PMC2139352  PMID: 4709270

Abstract

A large amount of experimental evidence has already been presented indicating the great importance of the cell-mediated immunity in the pathogenesis of the LCM virus infection in mice. In this laboratory a method which makes it possible to measure this cellular immunity quantitatively in vitro has been developed. The method is based on the determination of the radioisotope released after the interaction between specifically sensitized lymphocytes and syngeneic 1Cr-labeled LCM virus-infected target cells. By using this technique the time-course of the cell-mediated immunity has been established in acutely infected mice and in virus carriers adoptively immunized with syngeneic sensitized lymphocytes. Lymphocytes from acutely infected mice showed a strong lysing effect on the target cells, with a sharp maximum at about the 9th day after infection. The cell-mediated immunity in adoptively immunized virus carrier mice showed the same time-course, but in these animals the lytic effect of the lymphoid cells was considerably less pronounced. Lymphocytes from untreated virus carriers did not, however, have any effect on the target cells, and in these animals it was not possible to demonstrate any evidence of enhancing antibodies, In experiments employing serial dilutions of sensitized lymphocytes in normal cells a direct linear relationship between the number of sensitized lymphocytes and target cell destruction was found. These experiments seem to indicate that the underlying mechanism in the cytotoxic reaction is a direct cell-to-cell interaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brunner K. T., Mauel J., Cerottini J. C., Chapuis B. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology. 1968 Feb;14(2):181–196. [PMC free article] [PubMed] [Google Scholar]
  2. Brunner K. T., Mauel J., Rudolf H., Chapuis B. Studies of allograft immunity in mice. I. Induction, development and in vitro assay of cellular immunity. Immunology. 1970 Apr;18(4):501–515. [PMC free article] [PubMed] [Google Scholar]
  3. Canty T. G., Wunderlich J. R., Fletcher F. Qualitative and quantitative studies of cytotoxic immune cells. J Immunol. 1971 Jan;106(1):200–208. [PubMed] [Google Scholar]
  4. Granger G. A., Kolb W. P. Lymphocyte in vitro cytotoxicity: mechanisms of immune and non-immune small lymphocyte mediated target L cell destruction. J Immunol. 1968 Jul;101(1):111–120. [PubMed] [Google Scholar]
  5. Henney C. S. Quantitation of the cell-mediated immune response. I. The number of cytolytically active mouse lymphoid cells induced by immunization with allogeneic mastocytoma cells. J Immunol. 1971 Dec;107(6):1558–1566. [PubMed] [Google Scholar]
  6. Holtermann O. A., Majde J. A. An apparent histoincompatibility between mice chronically infected with lymphocytic choriomeningitis virus and their uninfected syngeneic counterparts. Transplantation. 1971 Jan;11(1):20–29. doi: 10.1097/00007890-197101000-00003. [DOI] [PubMed] [Google Scholar]
  7. Lundstedt C. Interaction between antigenically different cells. Virus-induced cytotoxicity by immune lymphoid cells in vitro. Acta Pathol Microbiol Scand. 1969;75(1):139–152. [PubMed] [Google Scholar]
  8. Oldstone M. B., Dixon F. J. Tissue injury in lymphocytic choriomeningitis viral infection: virus-induced immunologically specific release of a cytotoxic factor from immune lymphoid cells. Virology. 1970 Dec;42(4):805–813. doi: 10.1016/0042-6822(70)90330-2. [DOI] [PubMed] [Google Scholar]
  9. Oren M. E., Herberman R. B., Canty T. G. Immune response to Gross virus-induced lymphoma. II. Kinetics of the cellular immune response. J Natl Cancer Inst. 1971 Mar;46(3):621–628. [PubMed] [Google Scholar]
  10. Pedersen I. R., Volkert M. Multiplication of lymphocytic choriomeningitis virus in suspension cultures of Earle's strain L cells. Acta Pathol Microbiol Scand. 1966;67(4):523–536. doi: 10.1111/apm.1966.67.4.523. [DOI] [PubMed] [Google Scholar]
  11. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  12. Tosolini F. A., Mims C. A. Effect of murine strain and viral strain on the pathogenesis of lymphocytic choriomeningitis infection and a study of footpad responses. J Infect Dis. 1971 Feb;123(2):134–144. doi: 10.1093/infdis/123.2.134. [DOI] [PubMed] [Google Scholar]
  13. VOLKERT M., LARSEN J. H., PFAU C. STUDIES ON IMMUNOLOGICAL TOLERANCE TO LCM VIRUS. 4. THE QUESTION OF IMMUNITY IN ADOPTIVELY IMMUNIZED VIRUS CARRIERS. Acta Pathol Microbiol Scand. 1964;61:268–282. doi: 10.1111/apm.1964.61.2.268. [DOI] [PubMed] [Google Scholar]
  14. VOLKERT M., LARSEN J. H. STUDIES ON IMMUNOLOGICAL TOLERANCE TO LCM VIRUS. 3. DURATION AND MAXIMAL EFFECT OF ADOPTIVE IMMUNIZATION OF VIRUS CARRIERS. Acta Pathol Microbiol Scand. 1964;60:577–587. doi: 10.1111/apm.1964.60.4.577. [DOI] [PubMed] [Google Scholar]
  15. Volkert M., Larsen J. H. Immunological tolerance to viruses. Prog Med Virol. 1965;7:160–207. [PubMed] [Google Scholar]
  16. Volkert M., Lundstedt C. The provocation of latent lymphocytic choriomeningitis virus infections in mice by treatment with antilymphocytic serum. J Exp Med. 1968 Feb 1;127(2):327–339. doi: 10.1084/jem.127.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wortis H. H., Taylor R. B., Dresser D. W. Antibody production studied by means of the LHG assay. I. The splenic response of CBA mice to sheep erythrocytes. Immunology. 1966 Dec;11(6):603–616. [PMC free article] [PubMed] [Google Scholar]

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