Abstract
Epstein-Barr virus (EBV)-transformed autologous lymphoblasts were repeatedly inoculated into three squirrel monkeys. Each animal developed the heterophile antibodies of infectious mononucleosis and EBV-specific antibodies. After serologic responses had disappeared or markedly declined, the animals were challenged with either whole cells, cell filtrate, or cell ghosts. Animals challenged with living cells and cell ghosts developed agglutinin responses; the recipient of filtrate did not. The results suggest that EBV induces the appearance of the infectious mononucleosis heterophile antigen on the transformed cell membrane.
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- Adams R. A., Hellerstein E. E., Pothier L., Foley G. E., Lazarus H., Stuart A. B. Malignant potential of a cell line isolated from the peripheral blood in infectious mononucleosis. Cancer. 1971 Mar;27(3):651–658. doi: 10.1002/1097-0142(197103)27:3<651::aid-cncr2820270321>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
- Burger M. M. Forssman antigen exposed on surface membrane after viral transformation. Nat New Biol. 1971 May 26;231(21):125–126. doi: 10.1038/newbio231125a0. [DOI] [PubMed] [Google Scholar]
- Chang R. S., Golden H. D. Transformation of human leucocytes by throat washing from infectious mononucleosis patients. Nature. 1971 Dec 10;234(5328):359–360. doi: 10.1038/234359a0. [DOI] [PubMed] [Google Scholar]
- Diehl V., Henle G., Henle W., Kohn G. Demonstration of a herpes group virus in cultures of peripheral leukocytes from patients with infectious mononucleosis. J Virol. 1968 Jul;2(7):663–669. doi: 10.1128/jvi.2.7.663-669.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans A. S., Niederman J. C., McCollum R. W. Seroepidemiologic studies of infectious mononucleosis with EB virus. N Engl J Med. 1968 Nov 21;279(21):1121–1127. doi: 10.1056/NEJM196811212792101. [DOI] [PubMed] [Google Scholar]
- Gerber P., Branch J. W., Rosenblum E. N. Attempts to transmit infectious mononucleosis to rhesus monkeys and marmosets and to isolate herpes-like virus. Proc Soc Exp Biol Med. 1969 Jan;130(1):14–19. doi: 10.3181/00379727-130-33478. [DOI] [PubMed] [Google Scholar]
- Gerber P., Hamre D., Moy R. A., Rosenblum E. N. Infectious mononucleosis: complement-fixing antibodies to herpes-like virus associated with Burkitt lymphoma. Science. 1968 Jul 12;161(3837):173–175. doi: 10.1126/science.161.3837.173. [DOI] [PubMed] [Google Scholar]
- Henle G., Henle W., Diehl V. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc Natl Acad Sci U S A. 1968 Jan;59(1):94–101. doi: 10.1073/pnas.59.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leikola J., Aho K. Experimentally induced mononucleosis-like heterophile antibodies in man. Clin Exp Immunol. 1969 Jul;5(1):67–73. [PMC free article] [PubMed] [Google Scholar]
- Miller G., Niederman J. C., Stitt D. A. Infectious mononucleosis: appearance of neutralizing antibody to Epstein-Barr virus measured by inhibition of formation of lymphoblastoid cell lines. J Infect Dis. 1972 Apr;125(4):403–406. doi: 10.1093/infdis/125.4.403. [DOI] [PubMed] [Google Scholar]
- Miller G., Shope T., Lisco H., Stitt D., Lipman M. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc Natl Acad Sci U S A. 1972 Feb;69(2):383–387. doi: 10.1073/pnas.69.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Niederman J. C., McCollum R. W., Henle G., Henle W. Infectious mononucleosis. Clinical manifestations in relation to EB virus antibodies. JAMA. 1968 Jan 15;203(3):205–209. doi: 10.1001/jama.203.3.205. [DOI] [PubMed] [Google Scholar]