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. 1973 Jan 1;137(1):69–84. doi: 10.1084/jem.137.1.69

DIRECT DEMONSTRATION OF THETA-POSITIVE ANTIGEN-BINDING CELLS, WITH ANTIGEN-INDUCED MOVEMENT OF THYMUS-DEPENDENT CELL RECEPTORS

Robert F Ashman 1, Martin C Raff 1
PMCID: PMC2139368  PMID: 4569470

Abstract

Anti-θAKR antibody conjugated to fluorescein has been used in direct immunofluorescence tests to identify spleen θ+ (T) sheep erythrocyte rosette-forming cells in AKR mice. Specificity studies involving A and cogenic A/θAKR mice clearly demonstrated that the cell surface fluorescence and cytotoxicity produced by the antiserum is directed solely toward the θAKR alloantigen. Approximately ⅜ of rosette-forming and non-rosette-forming spleen cells were found to be θ+. The tendency for T cells to bind less antigen and the tendency for antigen-binding T cells to bear less θ than other spleen T cells, first suggested by other studies involving rosette-elimination by anti-θC3H plus complement, were confirmed by direct immunofluorescence. All AKR rosettes are specifically inhibitable by anti-immunoglobulin, including T rosettes. Antigen-induced redistribution of T cell receptors, analogous to that previously described for B cell receptors (16), occurs as readily in θ+RFC as in θ- RFC, without altering the symmetrical ring distribution of θAKR antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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