Abstract
Lymphocytes incubated with phytohemagglutinin or concanavalin A develop the capacity to stimulate autologous lymphocyte transformation. This is not attributable to residual mitogen contaminating the lymphoblastic cell preparation as: (a) the dissociation of mitogen from the lymphoblastic cell preparation increases the degree of stimulation observed and (b) the kinetics of lymphocyte transformation stimulated by phytohemagglutinin-induced lymphoblasts is different from that stimulated by phytohemagglutinin. The appearance of the stimulatory determinants on lymphocytes exposed to phytohemagglutinin precedes morphological transformation.
Full Text
The Full Text of this article is available as a PDF (545.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Birnbaum G., Siskind G. W., Weksler M. E. Autologous and allogeneic stimulation of peripheral human leukocytes. Cell Immunol. 1972 Jan;3(1):44–52. doi: 10.1016/0008-8749(72)90224-9. [DOI] [PubMed] [Google Scholar]
- Flier J. S., Glade P. R., Broder S. W., Hirschhorn K. Lymphocyte stimulation by allogeneic and autochthonous cultured lymphoid cells. Cell Immunol. 1970 Dec;1(6):596–602. doi: 10.1016/0008-8749(70)90025-0. [DOI] [PubMed] [Google Scholar]
- Fox T. O., Sheppard J. R., Burger M. M. Cyclic membrane changes in animal cells: transformed cells permanently display a surface architecture detected in normal cells only during mitosis. Proc Natl Acad Sci U S A. 1971 Jan;68(1):244–247. doi: 10.1073/pnas.68.1.244. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerber P. Activation of Epstein-Barr virus by 5-bromodeoxyuridine in "virus-free" human cells (complement-fixing antigen-immunofluorescence-leukocytes). Proc Natl Acad Sci U S A. 1972 Jan;69(1):83–85. doi: 10.1073/pnas.69.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerber P., Lucas S. J. In vitro stimulation of human lymphocytes by Epstein-Barr virus. Cell Immunol. 1972 Oct;5(2):318–324. doi: 10.1016/0008-8749(72)90057-3. [DOI] [PubMed] [Google Scholar]
- Green S. S., Sell K. W. Mixed leukocyte stimulation of normal peripheral leukocytes by autologous lymphoblastoid cells. Science. 1970 Nov 27;170(3961):989–990. doi: 10.1126/science.170.3961.989. [DOI] [PubMed] [Google Scholar]
- Hampar B., Derge J. G., Martos L. M., Walker J. L. Synthesis of Epstein-Barr virus after activation of the viral genome in a "virus-negative" human lymphoblastoid cell (Raji) made resistant to 5-bromodeoxyuridine (thymidine kinase-virus antigen-immunofluorescence-herpesvirus fingerprints). Proc Natl Acad Sci U S A. 1972 Jan;69(1):78–82. doi: 10.1073/pnas.69.1.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Han T., Moore G. E., Sokal J. E. In vitro lymphocyte response to autologous cultured lymphoid cells. Proc Soc Exp Biol Med. 1971 Mar;136(3):976–979. doi: 10.3181/00379727-136-35409. [DOI] [PubMed] [Google Scholar]
- Hardy D. A., Knight S., Ling N. R. The interaction of normal lymphocytes and cells from lymphoid cell lines. I. The nature of the activation process. Immunology. 1970 Aug;19(2):329–342. [PMC free article] [PubMed] [Google Scholar]
- Knight S. C., Moore G. E., Clarkson B. D. Stimulation of autochthonous lymphocytes by cells from normal and leukaemic lines. Nat New Biol. 1971 Feb 10;229(6):185–187. doi: 10.1038/newbio229185a0. [DOI] [PubMed] [Google Scholar]
- Nilsson K., Klein G., Henle W., Henle G. The establishment of lymphoblastoid lines from adult and fetal human lymphoid tissue and its dependence on EBV. Int J Cancer. 1971 Nov 15;8(3):443–450. doi: 10.1002/ijc.2910080312. [DOI] [PubMed] [Google Scholar]
- Nonoyama M., Pagano J. S. Detection of Epstein-Barr viral genome in nonproductive cells. Nat New Biol. 1971 Sep 22;233(38):103–106. doi: 10.1038/newbio233103a0. [DOI] [PubMed] [Google Scholar]
- Schwartz R. S. Immunoregulation, oncogenic viruses, and malignant lymphomas. Lancet. 1972 Jun 10;1(7763):1266–1269. doi: 10.1016/s0140-6736(72)90985-3. [DOI] [PubMed] [Google Scholar]
- Weksler M. E., Birnbasum G. Lymphocyte transformation induced by autologous cells: stimulation by cultured lymphoblast lines. J Clin Invest. 1972 Dec;51(12):3124–3132. doi: 10.1172/JCI107139. [DOI] [PMC free article] [PubMed] [Google Scholar]