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. 1973 Feb 28;137(3):821–837. doi: 10.1084/jem.137.3.821

DIFFERENTIATION OF THYMUS-DERIVED CELLS FROM PRECURSORS IN MOUSE BONE MARROW

M O El-Arini 1, D Osoba 1
PMCID: PMC2139385  PMID: 4266014

Abstract

An experimental model system was developed to study the differentiation of thymus-derived (T) cells from progenitors in bone marrow. In this system transplantation of bone marrow cells depleted of T cells gave rise to T cells in the spleens of irradiated recipients within 15 days of transplantation. Thus, normal bone marrow contains a class of cells that are progeniotrs of T cells (PT cells). PT cells are different from T cells since PT cells are incapable of responding to alloantigen, are θ resistant, and band in a lighter density region than do T cells. The density profile of PT cells is different from that of hematopoietic stem cells (CFU-S); PT cells band in a denser region of the gradient than do CFU-S.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach F. H., Albertini R. J., Joo P., Anderson J. L., Bortin M. M. Bone-marrow transplantation in a patient with the Wiskott-Aldrich syndrome. Lancet. 1968 Dec 28;2(7583):1364–1366. doi: 10.1016/s0140-6736(68)92672-x. [DOI] [PubMed] [Google Scholar]
  2. Davies A. J., Leuchars E., Wallis V., Koller P. C. The mitotic response of thymus-derived cells to antigenic stimulus. Transplantation. 1966 Jul;4(4):438–451. doi: 10.1097/00007890-196607000-00008. [DOI] [PubMed] [Google Scholar]
  3. De Koning J., Van Bekkum D. W., Dicke K. A., Dooren L. J., Rádl J., Van Rood J. J. Transplantation of bone-marrow cells and fetal thymus in an infant with lymphopenic immunological deficiency. Lancet. 1969 Jun 21;1(7608):1223–1227. doi: 10.1016/s0140-6736(69)92112-6. [DOI] [PubMed] [Google Scholar]
  4. Edwards G. E., Miller R. G., Phillips R. A. Differentiation of rosette-forming cells from myeloid stem cells. J Immunol. 1970 Sep;105(3):719–729. [PubMed] [Google Scholar]
  5. FORD C. E., HAMERTON J. L., BARNES D. W., LOUTIT J. F. Cytological identification of radiation-chimaeras. Nature. 1956 Mar 10;177(4506):452–454. doi: 10.1038/177452a0. [DOI] [PubMed] [Google Scholar]
  6. Gorczynski R. M., Miller R. G., Phillips R. A. Homogeneity of antibody-producing cells as analysed by their buoyant density in gradients of Ficoll. Immunology. 1970 Nov;19(5):817–829. [PMC free article] [PubMed] [Google Scholar]
  7. Gorczynski R. M., Miller R. G., Phillips R. A. Initiation of antibody production to sheep erythrocytes in vitro: replacement of the requirement for T-cells with a cell-free factor isolated from cultures of lymphoid cells. J Immunol. 1972 Feb;108(2):547–551. [PubMed] [Google Scholar]
  8. Lafleur L., Miller R. G., Phillips R. A. A quantitative assay for the progenitors of bone marrow-associated lymphocytes. J Exp Med. 1972 Jun 1;135(6):1363–1374. doi: 10.1084/jem.135.6.1363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Messner H., Till J. E., McCulloch E. A. Density distributions of marrow cells from mouse and man. Ser Haematol. 1972;5(2):22–36. [PubMed] [Google Scholar]
  10. Nowell P. C., Hirsch B. E., Fox D. H., Wilson D. B. Evidence for the existence of multipotential lympho-hematopoietic stem cells in adult rat. J Cell Physiol. 1970 Apr;75(2):151–158. doi: 10.1002/jcp.1040750203. [DOI] [PubMed] [Google Scholar]
  11. Nowell P. C., Wilson D. B. Lymphocytes and hemic stem cells. Am J Pathol. 1971 Dec;65(3):641–651. [PMC free article] [PubMed] [Google Scholar]
  12. TILL J. E., McCULLOCH E. A. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Res. 1961 Feb;14:213–222. [PubMed] [Google Scholar]
  13. VAN BEKKUM D. W., VOS O. Immunological aspects of homo- and heterologous bone marrow transplantation in irradiated animals. J Cell Physiol Suppl. 1957 Dec;50(Suppl 1):139–156. doi: 10.1002/jcp.1030500410. [DOI] [PubMed] [Google Scholar]
  14. VAN BEKKUMD THE SELECTIVE ELIMINATION OF IMMUNOLOGICALLY COMPETENT CELLS FROM BONE MARROW AND LYMPHATIC CELL MIXTURES. I. EFFECT OF STORAGE AT 4 C. Transplantation. 1964 May;2:393–404. doi: 10.1097/00007890-196405000-00009. [DOI] [PubMed] [Google Scholar]
  15. Worton R. G., McCulloch E. A., Till J. E. Physical separation of hemopoietic stem cells from cells forming colonies in culture. J Cell Physiol. 1969 Oct;74(2):171–182. doi: 10.1002/jcp.1040740209. [DOI] [PubMed] [Google Scholar]

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