Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 Nov;169(11):5304–5307. doi: 10.1128/jb.169.11.5304-5307.1987

Escherichia coli strains carrying the cloned cytochrome d terminal oxidase complex are sensitive to near-UV inactivation.

L J Sammartano 1, R W Tuveson 1
PMCID: PMC213941  PMID: 3312171

Abstract

To determine if membrane-bound cytochromes function as endogenous near-UV photosensitizers, strains containing the cloned cydA and cydB genes were tested for near-UV sensitivity. A strain containing both cloned genes overproduced cytochromes b558, b595, and d. Another strain containing only cloned cydB overproduced cytochrome b558. Both cytochrome-overproducing strains were hypersensitive to broad-spectrum near-UV inactivation. The presence of excess cytochromes did not affect sensitivity to far-UV radiation and provided protection against H2O2 inactivation.

Full text

PDF
5304

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bragg P. D. Effect of near-ultraviolet light on the respiratory chain of Escherichia coli. Can J Biochem. 1971 May;49(5):492–495. doi: 10.1139/o71-073. [DOI] [PubMed] [Google Scholar]
  2. Chamberlain J., Moss S. H. Lipid peroxidation and other membrane damage produced in Escherichia coli K1060 by near-UV radiation and deuterium oxide. Photochem Photobiol. 1987 May;45(5):625–630. doi: 10.1111/j.1751-1097.1987.tb07389.x. [DOI] [PubMed] [Google Scholar]
  3. Grabau C., Cronan J. E., Jr Molecular cloning of the gene (poxB) encoding the pyruvate oxidase of Escherichia coli, a lipid-activated enzyme. J Bacteriol. 1984 Dec;160(3):1088–1092. doi: 10.1128/jb.160.3.1088-1092.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Green G. N., Gennis R. B. Isolation and characterization of an Escherichia coli mutant lacking cytochrome d terminal oxidase. J Bacteriol. 1983 Jun;154(3):1269–1275. doi: 10.1128/jb.154.3.1269-1275.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Green G. N., Kranz J. E., Gennis R. B. Cloning the cyd gene locus coding for the cytochrome d complex of Escherichia coli. Gene. 1984 Dec;32(1-2):99–106. doi: 10.1016/0378-1119(84)90037-4. [DOI] [PubMed] [Google Scholar]
  6. Green G. N., Kranz R. G., Lorence R. M., Gennis R. B. Identification of subunit I as the cytochrome b558 component of the cytochrome d terminal oxidase complex of Escherichia coli. J Biol Chem. 1984 Jun 25;259(12):7994–7997. [PubMed] [Google Scholar]
  7. Green G. N., Lorence R. M., Gennis R. B. Specific overproduction and purification of the cytochrome b558 component of the cytochrome d complex from Escherichia coli. Biochemistry. 1986 May 6;25(9):2309–2314. doi: 10.1021/bi00357a002. [DOI] [PubMed] [Google Scholar]
  8. Ingledew W. J., Poole R. K. The respiratory chains of Escherichia coli. Microbiol Rev. 1984 Sep;48(3):222–271. doi: 10.1128/mr.48.3.222-271.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klamen D. L., Tuveson R. W. The effect of membrane fatty acid composition on the near-UV (300-400 nm) sensitivity of Escherichia coli K1060. Photochem Photobiol. 1982 Feb;35(2):167–173. doi: 10.1111/j.1751-1097.1982.tb03827.x. [DOI] [PubMed] [Google Scholar]
  10. Miller M. J., Gennis R. B. The purification and characterization of the cytochrome d terminal oxidase complex of the Escherichia coli aerobic respiratory chain. J Biol Chem. 1983 Aug 10;258(15):9159–9165. [PubMed] [Google Scholar]
  11. Moss S. H., Smith K. C. Membrane damage can be a significant factor in the inactivation of Escherichia coli by near-ultraviolet radiation. Photochem Photobiol. 1981 Feb;33(2):203–210. doi: 10.1111/j.1751-1097.1981.tb05325.x. [DOI] [PubMed] [Google Scholar]
  12. Peak M. J., Johnson J. S., Tuveson R. W., Peak J. G. Inactivation by monochromatic near-UV radiation of an Escherichia coli hemA8 mutant grown with and without delta-aminolevulinic acid: the role of DNA vs membrane damage. Photochem Photobiol. 1987 Apr;45(4):473–478. doi: 10.1111/j.1751-1097.1987.tb05405.x. [DOI] [PubMed] [Google Scholar]
  13. Sammartano L. J., Tuveson R. W., Davenport R. Control of sensitivity to inactivation by H2O2 and broad-spectrum near-UV radiation by the Escherichia coli katF locus. J Bacteriol. 1986 Oct;168(1):13–21. doi: 10.1128/jb.168.1.13-21.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sammartano L. J., Tuveson R. W. Escherichia coli xthA mutants are sensitive to inactivation by broad-spectrum near-UV (300- to 400-nm) radiation. J Bacteriol. 1983 Nov;156(2):904–906. doi: 10.1128/jb.156.2.904-906.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sammartano L. J., Tuveson R. W. Hydrogen peroxide induced resistance to broad-spectrum near-ultraviolet light (300-400 nm) inactivation in Escherichia coli. Photochem Photobiol. 1985 Mar;41(3):367–370. doi: 10.1111/j.1751-1097.1985.tb03499.x. [DOI] [PubMed] [Google Scholar]
  16. Sammartano L. J., Tuveson R. W. The effects of exogenous catalase on broad-spectrum near-UV (300-400 nm) treated Escherichia coli cells. Photochem Photobiol. 1984 Nov;40(5):607–612. doi: 10.1111/j.1751-1097.1984.tb05348.x. [DOI] [PubMed] [Google Scholar]
  17. Săsărman A., Chartrand P., Proschek R., Desrochers M., Tardif D., Lapointe C. Uroporphyrin-accumulating mutant of Escherichia coli K-12. J Bacteriol. 1975 Dec;124(3):1205–1212. doi: 10.1128/jb.124.3.1205-1212.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tuveson R. W., Jonas R. B. Genetic control of near-UV (300-400 NM) sensitivity independent of the recA gene in strains of Escherichia coli K12. Photochem Photobiol. 1979 Dec;30(6):667–676. doi: 10.1111/j.1751-1097.1979.tb07197.x. [DOI] [PubMed] [Google Scholar]
  19. Tuveson R. W., Sammartano L. J. Sensitivity of hemA mutant Escherichia coli cells to inactivation by near-UV light depends on the level of supplementation with delta-aminolevulinic acid. Photochem Photobiol. 1986 Jun;43(6):621–626. doi: 10.1111/j.1751-1097.1986.tb05637.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES