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. 1973 Sep 1;138(3):715–722. doi: 10.1084/jem.138.3.715

ACTIVATION OF THE ALTERNATE COMPLEMENT PATHWAY BY AUTOLOGOUS RED CELL STROMA

Thomas R Poskitt 1, H Philip Fortwengler Jr 1, Beverly J Lunskis 1
PMCID: PMC2139415  PMID: 4727918

Abstract

The present study demonstrates the ability of human autologous RBC stroma to activate the alternate complement pathway (C3-Activator system, properdin system), as evidenced by the generation of C3-Activator (C3A) from C3-Proactivator (C3PA) when RBC ghosts and sonicated ghosts are incubated with autologous serum. Intact RBC's, hemoglobin, and concentrated platelet stroma, on the other hand, are inactive in this regard. We postulate that this in vitro activity of RBC stroma may occur intravascularly when erythrocytes are damaged by immune or nonimmune mechanisms. The ensuing interaction of activated complement components with platelets leading to release of platelet factor three (PF-3) may constitute a mechanism for activation of the coagulation system during acute hemolytic episodes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbott D., Hussain S. Intravascular coagulation due to inter-donor incompatibility. Can Med Assoc J. 1970 Oct 10;103(7):752–753. [PMC free article] [PubMed] [Google Scholar]
  2. Amris C. J., Hansen N. E. Coagulation and fibrinolytic studies in paroxysmal nocturnal haemoglobinuria. Acta Med Scand. 1968 Dec;184(6):551–559. [PubMed] [Google Scholar]
  3. Birndorf N. I., Lopas H., Robboy S. J. Disseminated intravascular coagulation and renal failure: Production in the monkey with autologous red blood cell stroma. Lab Invest. 1971 Oct;25(4):314–319. [PubMed] [Google Scholar]
  4. Colman R. W., Robboy S. J., Minna J. D. Disseminated intravascular coagulation (DIC): an approach. Am J Med. 1972 May;52(5):679–689. doi: 10.1016/0002-9343(72)90058-7. [DOI] [PubMed] [Google Scholar]
  5. Dennis L. H., Eichelberger J. W., Inman M. M., Conrad M. E. Depletion of coagulation factors in drug-resistant Plasmodium falciparum malaria. Blood. 1967 May;29(5):713–721. [PubMed] [Google Scholar]
  6. Evensen S. A., Jeremic M. Platelets and the triggering mechanism of intravascular coagulation. Br J Haematol. 1970 Jul;19(1):33–46. doi: 10.1111/j.1365-2141.1970.tb01599.x. [DOI] [PubMed] [Google Scholar]
  7. Fong J. S., Good R. A. Prevention of the localized and generalized Shwartzman reactions by an anticomplementary agent, cobra venom factor. J Exp Med. 1971 Sep 1;134(3 Pt 1):642–655. doi: 10.1084/jem.134.3.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gewurz H., Shin H. S., Mergenhagen S. E. Interactions of the complement system with endotoxic lipopolysaccharide: consumption of each of the six terminal complement components. J Exp Med. 1968 Nov 1;128(5):1049–1057. doi: 10.1084/jem.128.5.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Henson P. M. Mechanisms of release of constituents from rabbit platelets by antigen-antibody complexes and complement. I. Lytic and nonlytic reactions. J Immunol. 1970 Aug;105(2):476–489. [PubMed] [Google Scholar]
  10. Lopas H., Birndorf N. I., Bell C. E., Jr, Robboy S. J., Fortwengler H. P., Biddison W. E. Experimental transfusion reactions in monkeys: haemolytic, coagulant and renal effects of transfused isoimmune IgG and IgM. Br J Haematol. 1972 Dec;23(6):765–776. doi: 10.1111/j.1365-2141.1972.tb03491.x. [DOI] [PubMed] [Google Scholar]
  11. Lopas H., Birndorf N. I. Haemolysis and intravascular coagulation due to incompatible red cell transfusion in isoimmunized monkeys. Br J Haematol. 1971 Oct;21(4):399–411. doi: 10.1111/j.1365-2141.1971.tb02700.x. [DOI] [PubMed] [Google Scholar]
  12. Lopas H., Birndorf N. I., Robboy S. J. Experimental transfusion reactions and disseminated intravascular coagulation produced by incompatible plasma in monkeys. Transfusion. 1971 Jul-Aug;11(4):196–203. doi: 10.1111/j.1537-2995.1971.tb04401.x. [DOI] [PubMed] [Google Scholar]
  13. Margaretten W., McKay D. G. The role of the platelet in the generalized Shwartzman reaction. J Exp Med. 1969 Mar 1;129(3):585–590. doi: 10.1084/jem.129.3.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. ROBBINS J., STETSON C. A., Jr An effect of antigen-antibody interaction on blood coagulation. J Exp Med. 1959 Jan 1;109(1):1–8. doi: 10.1084/jem.109.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rabiner S. F., Friedman L. H. The role of intravascular haemolysis and the reticulo-endothelial system in the production of a hypercoagulable state. Br J Haematol. 1968 Feb;14(2):105–118. doi: 10.1111/j.1365-2141.1968.tb01480.x. [DOI] [PubMed] [Google Scholar]
  16. Rodríguez-Erdmann F. Intravascular activation of the clotting system with phospholipids. Production of the generalized Shwartzman reaction with platelet factor 3. Blood. 1965 Nov;26(5):541–553. [PubMed] [Google Scholar]
  17. Sack E. S., Nefa O. M. Fibrinogen and fibrin degradation products in hemolytic transfusion reactions. Transfusion. 1970 Nov-Dec;10(6):317–321. doi: 10.1111/j.1537-2995.1970.tb00751.x. [DOI] [PubMed] [Google Scholar]
  18. Tellez A., Rubinstein P. Rapid method for separation of blood cells. Transfusion. 1970 Jul-Aug;10(4):223–225. doi: 10.1111/j.1537-2995.1970.tb00733.x. [DOI] [PubMed] [Google Scholar]
  19. Zimmerman T. S., Arroyove C. M., Müller-Eberhard H. J. A blood coagulation abnormality in rabbits deficient in the sixth component of complement (C6) and its correction by purified C6. J Exp Med. 1971 Dec 1;134(6):1591–1600. doi: 10.1084/jem.134.6.1591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zimmerman T. S., Müller-Eberhard H. J. Blood coagulation initiation by a complement-mediated pathway. J Exp Med. 1971 Dec 1;134(6):1601–1607. doi: 10.1084/jem.134.6.1601. [DOI] [PMC free article] [PubMed] [Google Scholar]

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