Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1973 Sep 1;138(3):522–537. doi: 10.1084/jem.138.3.522

DETECTION OF IDIOTYPIC CROSS-REACTIONS AMONG STREPTOCOCCAL ANTISERA FROM RELATED RABBITS

Thomas J Kindt 1, Rochelle K Seide 1, Viktor A Bokisch 1, Richard M Krause 1
PMCID: PMC2139422  PMID: 4727913

Abstract

Idiotypic cross-reactions among antibodies to Group C streptococcal carbohydrate were studied using idiotypic antisera prepared in allotypically matched rabbits. Antibodies with idiotypic cross-specificity to one proband antibody were detected in 58% of the antisera from related rabbits, while approximately 1% of nonrelated rabbits produced antibody with this specificity. The cross-specificity was related to the group a (VH) allotype of 133 rabbits tested with only one exception. Studies utilizing antisera against a second proband antibody failed to detect antibodies with idiotypic cross-reactivity among the same group of related rabbits. This result emphasizes the variation in expression of idiotypic determinants of antibodies. It was further shown that the presence of anti-IgG's in the streptococcal antisera interfere with the detection of idiotypic cross-reactions. These anti-IgG's masked the presence of antibodies with idiotypic cross-specificity when inhibition of precipitation tests were used for their detection.

Full Text

The Full Text of this article is available as a PDF (881.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S., Ternynck T. Biologically active water-insoluble protein polymers. I. Their use for isolation of antigens and antibodies. J Biol Chem. 1967 Apr 10;242(7):1651–1659. [PubMed] [Google Scholar]
  2. Bokisch V. A., Bernstein D., Krause R. M. Occurrence of 19S and 7S anti-IgGs during hyperimmunization of rabbits with streptococci. J Exp Med. 1972 Oct 1;136(4):799–815. doi: 10.1084/jem.136.4.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brient B. W., Haimovich J., Nisonoff A. Reaction of anti-idiotypic antibody with the hapten-binding site of a myeloma protein. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3136–3139. doi: 10.1073/pnas.68.12.3136. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Briles D. E., Krause R. M. Mouse antibodies to group A streptococcal carbohydrate; use of idiotypy to detect inbred strain specificity and to monitor spleen cell transfer in syngenetic mice. J Immunol. 1972 Dec;109(6):1311–1320. [PubMed] [Google Scholar]
  5. Carson D., Weigert M. Immunochemical analysis of the cross-reacting idiotypes of mouse myeloma proteins with anti-dextran activity and normal anti-dextran antibody. Proc Natl Acad Sci U S A. 1973 Jan;70(1):235–239. doi: 10.1073/pnas.70.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Daugharty H., Hopper J. E., MacDonald A. B., Nisonoff A. Quantitative investigations of idiotypic antibodies. I. Analysis of precipitating antibody populations. J Exp Med. 1969 Nov 1;130(5):1047–1062. doi: 10.1084/jem.130.5.1047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eichmann K. Idiotype expression and the inheritance of mouse antibody clones. J Exp Med. 1973 Mar 1;137(3):603–621. doi: 10.1084/jem.137.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eichmann K., Kindt T. J. The inheritance of individual antigenic specificities of rabbit antibodies to streptococcal carbohydrates. J Exp Med. 1971 Aug 1;134(2):532–552. doi: 10.1084/jem.134.2.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FLEISCHMAN J. B., PORTER R. R., PRESS E. M. THE ARRANGEMENT OF THE PEPTIDE CHAINS IN GAMMA-GLOBULIN. Biochem J. 1963 Aug;88:220–228. doi: 10.1042/bj0880220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fleischman J. B. A partial amino acid sequence in the heavy chain of a rabbit antibody to group C streptococcal carbohydrate. Biochemistry. 1971 Jul 6;10(14):2753–2761. doi: 10.1021/bi00790a016. [DOI] [PubMed] [Google Scholar]
  11. GREY H. M., MANNIK M., KUNKEL H. G. INDIVIDUAL ANTIGENIC SPECIFICITY OF MYELOMA PROTEINS. CHARACTERISTICS AND LOCALIZATION TO SUBUNITS. J Exp Med. 1965 Apr 1;121:561–575. doi: 10.1084/jem.121.4.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gold E. R., Fudenberg H. H. Chromic chloride: a coupling reagent for passive hemagglutination reactions. J Immunol. 1967 Nov;99(5):859–866. [PubMed] [Google Scholar]
  13. Kindt T. J., Klapper D. G., Waterfield M. D. An idiotypic cross-reaction between allotype a3 and allotype a negative rabbit antibodies to streptococcal carbohydrate. J Exp Med. 1973 Mar 1;137(3):636–648. doi: 10.1084/jem.137.3.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kindt T. J., Seide R. K., Lackland H., Thunberg A. L. Serologic identity of the b4 allotypic determinants present on homogeneous rabbit light chains with different N-terminal amino acid sequences. J Immunol. 1972 Oct;109(4):735–741. [PubMed] [Google Scholar]
  15. Kunkel H. G., Agnello V., Joslin F. G., Winchester R. J., Capra J. D. Cross-idiotypic specificity among monoclonal IgM proteins with anti- -globulin activity. J Exp Med. 1973 Feb 1;137(2):331–342. doi: 10.1084/jem.137.2.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  17. Mole L. E., Jackson S. A., Porter R. R., Wilkinson J. M. Allotypically related sequences in the Fd fragment of rabbit immunoglobulin heavy chains. Biochem J. 1971 Sep;124(2):301–318. doi: 10.1042/bj1240301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Oudin J., Cazenave P. A. Similar idiotypic specificities in immunoglobulin fractions with different antibody functions or even without detectable antibody function. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2616–2620. doi: 10.1073/pnas.68.10.2616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pawlak L. L., Mushinski E. B., Nisonoff A., Potter M. Evidence for the linkage of the IGC H locus to a gene controlling the idiotypic specificity of anti-p-azophenylarsonate antibodies in strain A mice. J Exp Med. 1973 Jan 1;137(1):22–31. doi: 10.1084/jem.137.1.22. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Willims R. C., Jr, Kunkel H. G., Capra J. D. Antigenic specificities related to the cold agglutinin activity of gamma M globulins. Science. 1968 Jul 26;161(3839):379–381. doi: 10.1126/science.161.3839.379. [DOI] [PubMed] [Google Scholar]
  21. Winfield J. B., Pincus J. H., Mage R. G. Persistence and characterization of idiotypes in pedigreed rabbits producing antibodies of restricted heterogeneity to pneumococcal polysaccharides. J Immunol. 1972 May;108(5):1278–1287. [PubMed] [Google Scholar]
  22. Wu T. T., Kabat E. A. An analysis of the sequences of the variable regions of Bence Jones proteins and myeloma light chains and their implications for antibody complementarity. J Exp Med. 1970 Aug 1;132(2):211–250. doi: 10.1084/jem.132.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES