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. 1973 Oct 31;138(5):1065–1076. doi: 10.1084/jem.138.5.1065

REQUIREMENT FOR α-L-FUCOSE ON THE MACROPHAGE MEMBRANE RECEPTOR FOR MIF

Heinz G Remold 1
PMCID: PMC2139444  PMID: 4583070

Abstract

α-L-fucose abolishes the activity of guinea pig migration inhibitory factor (MIF) on the macrophages. Other sugars such as α-D-glucose, β-D-galactose, α-L-rhamnose, methyl-α-D-mannoside, and N-acetyl-β-D-glucosamine had no effect. Theabolition of MIF activity by α-L-fucose was reversible. When macrophages were incubated with α-L-fucosidase, a glycosidase which splits terminal α-L-fucose from oligosaccharides, the macrophages no longer responded to MIF. On the other hand, MIF incubated with α-L-fucosidase was still active. These experiments strongly suggest that α-L-fucose comprises an essential part of a macrophage membrane receptor for MIF.

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Selected References

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