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. 1973 Oct 31;138(5):1230–1247. doi: 10.1084/jem.138.5.1230

INTERACTION OF HUMAN THYMUS-DERIVED AND NON-THYMUS-DERIVED LYMPHOCYTES IN VITRO

INDUCTION OF PROLIFERATION AND ANTIBODY SYNTHESIS IN B LYMPHOCYTES BY A SOLUBLE FACTOR RELEASED FROM ANTIGEN-STIMULATED T LYMPHOCYTES

Raif S Geha 1, Eveline Schneeberger 1, Fred S Rosen 1, Ezio Merler 1
PMCID: PMC2139447  PMID: 4200776

Abstract

Relatively pure populations of human T and B lymphocytes were obtained from blood and tonsils using density gradient centrifugation in bovine serum albumin. Antigen alone was incapable of triggering the B lymphocyte into blast transformation or to secrete antibody. However, supernatants from tetanus toxoid-stimulated T cells obtained from immune donors contained a factor mitogenic for B lymphocytes. 50–60% of B cells responded to this lymphocyte mitogenic factor (LMF) by proliferation, loss of C3 reactivity, and change to a secretory state. LMF-stimulated B cells exhibited a three- to fivefold increase in protein secretion and a six- to eightfold increase in gamma G globulin secretion. De novo secreted IgG had specificity directed to the tetanus toxoid present in the LMF containing T-cell supernatants. This was confirmed by an increase in the number of indirect plaque-forming cells to tetanus toxoid-coated sheep red blood cells after stimulation of B cells with LMF. It is proposed that in the course of the response to a previously encountered protein antigen, sensitized human T cells emit a signal in the form of a soluble product that, together with antigen, triggers B cells into division and antibody secretion. The experimental model utilized can be adapted to study human T-B cell cooperation under various conditions in normal individuals and in individuals with immunodeficiency diseases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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