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. 1973 Nov 30;138(6):1365–1378. doi: 10.1084/jem.138.6.1365

SURFACE MARKERS ON HUMAN B AND T LYMPHOCYTES

II. PRESENCE OF EPSTEIN-BARR VIRUS RECEPTORS ON B LYMPHOCYTES

Mikael Jondal 1, George Klein 1
PMCID: PMC2139459  PMID: 4357682

Abstract

Human peripheral lymphocytes were investigated for receptors binding Epstein-Barr virus (EBV) because of the regular association of this virus with infectious mononucleosis and Burkitt's lymphoma. This was done by a cytoadherence technique where virus-producing cells, displaying fresh viral determinants in their cytoplasmatic membrane, were mixed with lymphocytes. Unfractionated lymphocytes were found to adhere to these cells in contrast to column-purified T lymphocytes. The specificity of the binding was confirmed by blocking experiments that showed that sera containing high titers of antibodies directed against the virus could partially inhibit the adherence in contrast to low-titer sera. It is concluded that B lymphocytes, in contrast to T lymphocytes, have receptors for EBV. In a second line of experiments it was found that established human lymphoblastoid lines that carry the EBV genome had receptors characteristic for B lymphocytes and did not form T-lymphocyte rosettes. In contrast, a line of known T-lymphocyte origin that did not carry the EBV genome had receptors characteristic for T lymphocytes. EBV-transformed simian lymphoblastoid lines had surface markers indicating a B-lymphocyte origin in contrast to HVS-transformed simian lines that lacked surface immunoglobulin but carried receptors for sheep red blood cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bankhurst A. D., Warner N. L., Sprent J. Surface immunoglobulins on thymus and thymus-derived lymphoid cells. J Exp Med. 1971 Oct 1;134(4):1005–1015. doi: 10.1084/jem.134.4.1005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Basten A., Miller J. F., Sprent J., Pye J. A receptor for antibody on B lymphocytes. I. Method of detection and functional significance. J Exp Med. 1972 Mar 1;135(3):610–626. doi: 10.1084/jem.135.3.610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gergely L., Klein G., Ernberg I. The action of DNA antagonists on Epstein-Barr virus (EBV)-associated early antigen (EA) in Burkitt lymphoma lines. Int J Cancer. 1971 Mar 15;7(2):293–302. doi: 10.1002/ijc.2910070214. [DOI] [PubMed] [Google Scholar]
  4. Gunvén P., Klein G. Blocking of direct membrane immunofluorescence in titration of membrane-reactive antibodies associated with Epstein-Barr virus. J Natl Cancer Inst. 1971 Sep;47(3):539–548. [PubMed] [Google Scholar]
  5. Henle G., Henle W. Immunofluorescence in cells derived from Burkitt's lymphoma. J Bacteriol. 1966 Mar;91(3):1248–1256. doi: 10.1128/jb.91.3.1248-1256.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Henle W., Henle G., Zajac B. A., Pearson G., Waubke R., Scriba M. Differential reactivity of human serums with early antigens induced by Epstein-Barr virus. Science. 1970 Jul 10;169(3941):188–190. doi: 10.1126/science.169.3941.188. [DOI] [PubMed] [Google Scholar]
  7. Hinuma Y., Grace J. T., Jr Cloning of immunoglobulin-producing human leukemic and lymphoma cells in long-term cultures. Proc Soc Exp Biol Med. 1967 Jan;124(1):107–111. doi: 10.3181/00379727-124-31677. [DOI] [PubMed] [Google Scholar]
  8. Hämmerling U., Rajewsky K. Evidence for surface-associated immunoglobulin on T and B lymphocytes. Eur J Immunol. 1971 Dec;1(6):447–452. doi: 10.1002/eji.1830010608. [DOI] [PubMed] [Google Scholar]
  9. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klein E., Klein G., Nadkarni J. S., Nadkarni J. J., Wigzell H., Clifford P. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 1968 Jul;28(7):1300–1310. [PubMed] [Google Scholar]
  11. Klein G., Dombos L., Gothoskar B. Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus. Int J Cancer. 1972 Jul 15;10(1):44–57. doi: 10.1002/ijc.2910100108. [DOI] [PubMed] [Google Scholar]
  12. Klein G., Gergely L., Goldstein G. Two-colour immunofluorescence studies on EBV-determined antigens. Clin Exp Immunol. 1971 Apr;8(4):593–602. [PMC free article] [PubMed] [Google Scholar]
  13. Klein G. Herpesviruses and oncogenesis. Proc Natl Acad Sci U S A. 1972 Apr;69(4):1056–1064. doi: 10.1073/pnas.69.4.1056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marchalonis J. J., Atwell J. L., Cone R. E. Isolation of surface immunoglobulin from lymphocytes from human and murine thymus. Nat New Biol. 1972 Feb 23;235(60):240–242. doi: 10.1038/newbio235240a0. [DOI] [PubMed] [Google Scholar]
  15. Minowada J., Onuma T., Moore G. E. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Natl Cancer Inst. 1972 Sep;49(3):891–895. [PubMed] [Google Scholar]
  16. Nishioka K. Complement and tumor immunology. Adv Cancer Res. 1971;14:231–293. doi: 10.1016/s0065-230x(08)60522-9. [DOI] [PubMed] [Google Scholar]
  17. Purchase H. G. Virus-specific immunofluorescent and precipitin antigens and cell-free virus in the tissues of birds infected with Marek's disease. Cancer Res. 1970 Jun;30(6):1898–1908. [PubMed] [Google Scholar]
  18. Ross G. D., Rabellino E. M., Polley M. J., Grey H. M. Combined studies of complement receptor and surface immunoglobulin-bearing cells and sheep erythrocyte rosette-forming cells in normal and leukemic human lymphocytes. J Clin Invest. 1973 Feb;52(2):377–385. doi: 10.1172/JCI107194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Silvestre D., Kourilsky F. M., Klein G., Yata Y., Neauport-Sautes C., Levy J. P. Relationship between the EBV-associated membrane antigen on Burkitt lymphoma cells and the viral envelope, demonstrated by immunoferritin labelling. Int J Cancer. 1971 Sep 15;8(2):222–233. doi: 10.1002/ijc.2910080206. [DOI] [PubMed] [Google Scholar]
  20. Wigzell H., Sundqvist K. G., Yoshida T. O. Separation of cells according to surface antigens by the use of antibody-coated columns. Fractionation of cells carrying immunoglobulins and blood group antigen. Scand J Immunol. 1972;1(1):75–87. doi: 10.1111/j.1365-3083.1972.tb03737.x. [DOI] [PubMed] [Google Scholar]
  21. Yoshida T. O., Andersson B. Evidence for a receptor recognizing antigen complexed immunoglobulin on the surface of activated mouse thymus lymphocytes. Scand J Immunol. 1972;1(4):401–408. doi: 10.1111/j.1365-3083.1972.tb03306.x. [DOI] [PubMed] [Google Scholar]

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