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. 1973 Jan 31;137(2):239–253. doi: 10.1084/jem.137.2.239

SYNERGY AMONG LYMPHOID CELLS MEDIATING THE GRAFT-VERSUS-HOST RESPONSE

V. DERIVATION BY MIGRATION IN LETHALLY IRRADIATED RECIPIENTS OF TWO INTERACTING SUBPOPULATIONS OF THYMUS-DERIVED CELLS FROM NORMAL SPLEEN

Robert E Tigelaar 1, Richard Asofsky 1
PMCID: PMC2139477  PMID: 4405532

Abstract

Spleen cells from normal adult mice were injected into lethally irradiated adult syngeneic recipients. 24 h later, cell suspensions were prepared from the recipients' spleens or peripheral lymph nodes and tested either alone or combined for their capacity to elicit graft-versus-host (GVH) reactions in neonatal F1 recipients, using the Simonsen spleen weight assay. Either the lymph node-seeking subpopulation or the spleen-seeking subpopulation alone was markedly deficient in its ability to provide a GVH reaction when compared with the normal population from which it was derived. However, an appropriate mixture of the two had a reactivity characteristic of the parent population. Both subpopulations were sensitive to treatment with anti-θ antibody and complement in vitro. These results provide a convincing demonstration of the functional heterogeneity within the pool of thymus-derived cells present in a single normal lymphoid tissue. They strongly suggest that the normal expression of GVH reactivity of such a tissue involves an interaction among distinct subpopulations of thymus-derived cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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