Abstract
The ultrastructure of binding sites in rosette-forming cells of mice after immunization with sheep red cells was studied by means of scanning and transmission electron microscopy. It was found that the red cells were bound to the lymphocyte surface in circumscribed, immunoglobulin-containing areas, consistent with a spotlike or patchy distribution of antigen-binding immunoglobulin receptors. In these contact areas the cell membranes formed a gap of 80 Å (range 75–90 Å) which exhibited electron-opaque bridges at high magnification. These results are discussed in the light of the recent recognition of the formation of immunoglobulin spots on the lymphocyte surface after antigen contact. Morphological details suggest that the same mechanism is operating in rosette formation, possibly including the movement of the contact areas on the lymphocyte membrane.
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Selected References
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- Biberfeld P., Biberfeld G., Perlmann P. Surface immunoglobulin light chain determinants on normal and PHA-stimulated human blood lymphocytes studied by immunofluorescence and electronmicroscopy. Exp Cell Res. 1971 May;66(1):177–189. doi: 10.1016/s0014-4827(71)80027-7. [DOI] [PubMed] [Google Scholar]
- Biozzi G., Stiffel C., Mouton D., Bouthillier Y., Decreusefond C. Etude des cellules productrices d'anticorps par la méthode de l'immuno-cyto-adhérence. Pathol Biol. 1967 Apr;15(7):402–409. [PubMed] [Google Scholar]
- Clarke J. A., Salsbury A. J., Willoughby D. A. Some scanning electron-microscope observations on stimulated lymphocytes. J Pathol. 1971 Jun;104(2):115–118. doi: 10.1002/path.1711040205. [DOI] [PubMed] [Google Scholar]
- Gonatas N. K., Antoine J. C., Stieber A., Avrameas S. Surface immunoglobulins of thymus and lymph node cells demonstrated by the peroxidase coupling technique. Lab Invest. 1972 Mar;26(3):253–261. [PubMed] [Google Scholar]
- Gudat F. G., Harris T. N., Harris S., Hummeler K. Studies on antibody-producing cells. I. Ultrastructure of 19S and 7S antibody-producing cells. J Exp Med. 1970 Sep 1;132(3):448–474. doi: 10.1084/jem.132.3.448. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gudat F. G., Harris T. N., Harris S., Hummeler K. Studies on antibody-producing cells. II. Appearance of 3 H-thymidine-labeled rosette-forming cells. J Exp Med. 1971 Feb 1;133(2):305–320. doi: 10.1084/jem.133.2.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loor F., Forni L., Pernis B. The dynamic state of the lymphocyte membrane. Factors affecting the distribution and turnover of surface immunoglobulins. Eur J Immunol. 1972 Jun;2(3):203–212. doi: 10.1002/eji.1830020304. [DOI] [PubMed] [Google Scholar]
- Mandel T., Byrt P., Ada G. L. A morphological examination of antigen reactive cells from mouse spleen and peritoneal cavity. Exp Cell Res. 1969 Nov;58(1):179–182. doi: 10.1016/0014-4827(69)90132-3. [DOI] [PubMed] [Google Scholar]
- McConnell I., Munro A., Gurner B. W., Coombs R. R. Studies on actively allergized cells. I. The cyto-dynamics and morphology of rosete-forming lymph node cells in mice and inhibition of rosette-formation with antibody to mouse immunoglobulins. Int Arch Allergy Appl Immunol. 1969;35(3):209–227. [PubMed] [Google Scholar]
- NOTA N. R., LIACOPOULOS-BRIOT M., STIFFEL C., BIOZZI G. L'IMMUNO-CYTO-ADH'ERENCE: UNE M'ETHODE SIMPLE ET QUANTITATIVE POUR L'ETUDE IN VITRO DES CELLULES PRODUCTRICES D'ANTICORPS. C R Hebd Seances Acad Sci. 1964 Aug 3;259:1277–1280. [PubMed] [Google Scholar]
- Perkins W. D., Karnovsky M. J., Unanue E. R. An ultrastructural study of lymphocytes with surface-bound immunoglobulin. J Exp Med. 1972 Feb 1;135(2):267–276. doi: 10.1084/jem.135.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pernis B., Forni L., Amante L. Immunoglobulin spots on the surface of rabbit lymphocytes. J Exp Med. 1970 Nov;132(5):1001–1018. doi: 10.1084/jem.132.5.1001. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raff M. C., Sternberg M., Taylor R. B. Immunoglobulin determinants on the surface of mouse lymphoid cells. Nature. 1970 Feb 7;225(5232):553–554. doi: 10.1038/225553a0. [DOI] [PubMed] [Google Scholar]
- Santer V., Bankhurst A. D., Nossal G. J. Ultrastructural distribution of surface immunoglobulin determinants on mouse lymphoid cells. Exp Cell Res. 1972 Jun;72(2):377–386. doi: 10.1016/0014-4827(72)90005-5. [DOI] [PubMed] [Google Scholar]
- Storb U., Bauer W., Storb R., Fliedner T. M., Weiser R. S. Ultrastructure of rosette-forming cells in the mouse during the antibody response. J Immunol. 1969 Jun;102(6):1474–1485. [PubMed] [Google Scholar]
- Takahashi T., Old L. J., McIntire K. R., Boyse E. A. Immunoglobulin and other surface antigens of cells of the immune system. J Exp Med. 1971 Oct 1;134(4):815–832. doi: 10.1084/jem.134.4.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Warfel A. H., Elberg S. S. Macrophage membranes viewed through a scanning electron microscope. Science. 1970 Oct 23;170(3956):446–447. doi: 10.1126/science.170.3956.446. [DOI] [PubMed] [Google Scholar]
- ZAALBERG O. B. A SIMPLE METHOD FOR DETECTING SINGLE ANTIBODY-FORMING CELLS. Nature. 1964 Jun 20;202:1231–1231. doi: 10.1038/2021231a0. [DOI] [PubMed] [Google Scholar]
- Zaalberg O. B., van der Meul V. A., van Twisk M. J. Antibody production by isolated spleen cells: a study of the cluster and the plaque techniques. J Immunol. 1968 Feb;100(2):451–458. [PubMed] [Google Scholar]
- Zucker-Franklin D., Berney S. Electron microscope study of surface immunoglobulin-bearing human tonsil cells. J Exp Med. 1972 Mar 1;135(3):533–548. doi: 10.1084/jem.135.3.533. [DOI] [PMC free article] [PubMed] [Google Scholar]