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. 1974 Apr 1;139(4):1002–1012. doi: 10.1084/jem.139.4.1002

BINDING OF AGGREGATED γ-GLOBULIN TO ACTIVATED T LYMPHOCYTES IN THE GUINEA PIG

John A van Boxel 1, David L Rosenstreich 1
PMCID: PMC2139564  PMID: 4131510

Abstract

Heat-aggregated guinea pig γ-globulin was shown to bind to the surface membrane of a subclass of guinea pig T lymphocytes. Cells of this subpopulation were identified as T lymphocytes because these cells did not stain for surface Ig (a B-cell marker) but did form spontaneous E-rosettes with rabbit erythrocytes (a T-cell marker). A strikingly high proportion of such aggregate-binding (Agg+), E-rosette-forming (E-rosette+), but surface Ig-negative (Ig-) cells were found in an inflammatory exudate. Thus purified peritoneal exudate lymphocytes (PELs) are known to consist of over 90% T cells, and 59% of these cells bound aggregates. 10% of these Agg+ Ig- E-rosette+ cells were found in draining lymph node cell populations and none in thymus cell populations. The high frequency amongst PELs suggested that these Aggregate+ Ig- E-rosette+ cells might be activated T cells as these are known to occur in high proportion in PEL populations. Confirmatory evidence for this postulate was provided by the striking increase (from 10% to 46%) of Ig- E-rosette+ cells that bound aggregates when lymph node cells were activated by antigen stimulation in vitro.

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Selected References

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  1. Aström K. E., Webster H. D., Arnason B. G. The initial lesion in experimental allergic neuritis. A phase and electron microscopic study. J Exp Med. 1968 Sep 1;128(3):469–495. doi: 10.1084/jem.128.3.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Basten A., Warner N. L., Mandel T. A receptor for antibody on B lymphocytes. II. Immunochemical and electron microscopy characteristics. J Exp Med. 1972 Mar 1;135(3):627–642. doi: 10.1084/jem.135.3.627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bloom B. R., Chase M. W. Transfer of delayed-type hypersensitivity. A critical review and experimental study in the guinea pig. Prog Allergy. 1967;10:151–255. [PubMed] [Google Scholar]
  4. Dickler H. B., Kunkel H. G. Interaction of aggregated -globulin with B lymphocytes. J Exp Med. 1972 Jul 1;136(1):191–196. doi: 10.1084/jem.136.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dickler H. B., Siegal F. P., Bentwich Z. H., Kunkel H. G. Lymphocyte binding of aggregated IgG and surface Ig staining in chronic lymphocytic leukaemia. Clin Exp Immunol. 1973 May;14(1):97–106. [PMC free article] [PubMed] [Google Scholar]
  6. Grey H. M., Kubo R. T., Cerottini J. C. Thymus-derived (T) cell immunoglobulins. Presence of a receptor site for IgG and absence of large amounts of "buried" Ig determinants on T cells. J Exp Med. 1972 Nov 1;136(5):1323–1328. doi: 10.1084/jem.136.5.1323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Koster F. T., McGregor D. D., Mackaness G. B. The mediator of cellular immunity. II. Migration of immunologically committed lymphocytes into inflammatory exudates. J Exp Med. 1971 Feb 1;133(2):400–409. doi: 10.1084/jem.133.2.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Koster F., McGregor D. D. Rat thoracic duct lymphocytes: types that participate in inflammation. Science. 1970 Feb 20;167(3921):1137–1139. doi: 10.1126/science.167.3921.1137. [DOI] [PubMed] [Google Scholar]
  9. Lay W. H., Nussenzweig V. Receptors for complement of leukocytes. J Exp Med. 1968 Nov 1;128(5):991–1009. doi: 10.1084/jem.128.5.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McFarland W., Heilman D. H., Moorhead J. F. Functional anatomy of the lymphocyte in immunological reactions in vitro. J Exp Med. 1966 Nov 1;124(5):851–858. doi: 10.1084/jem.124.5.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McGregor D. D., Koster F. T., Mackaness G. B. The mediator of cellular immunity. I. The life-span and circulation dynamics of the immunologically committed lymphocyte. J Exp Med. 1971 Feb 1;133(2):389–399. doi: 10.1084/jem.133.2.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Oppenheim J. J., Zbar B., Rapp H. Specific inhibition of tumor cell DNA synthesis in vitro by lymphocytes from peritoneal exudate of immunized syngeneic guinea pigs. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1119–1126. doi: 10.1073/pnas.66.4.1119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pick E., Brostoff J., Krejci J., Turk J. L. Interaction between "sensitized lymphocytes" and antigen in vitro. II. Mitogen-induced release of skin reactive and macrophage migration inhibitory factors. Cell Immunol. 1970 May;1(1):92–109. doi: 10.1016/0008-8749(70)90063-8. [DOI] [PubMed] [Google Scholar]
  14. Roos D., Loos J. A. Changes in the carbohydrate metabolism of mitogenically stimulated human peripheral lymphocytes. I. Stimulation by phytohaemagglutinin. Biochim Biophys Acta. 1970 Dec 29;222(3):565–582. doi: 10.1016/0304-4165(70)90182-0. [DOI] [PubMed] [Google Scholar]
  15. Rosenstreich D. L., Blake J. T., Rosenthal A. S. The peritoneal exudate lymphocyte. I. Differences in antigen responsiveness between peritoneal exudate and lymph node lymphocytes from immunized guinea pigs. J Exp Med. 1971 Nov 1;134(5):1170–1186. doi: 10.1084/jem.134.5.1170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rosenstreich D. L., Shevach E., Green I., Rosenthal A. S. The uropod-bearing lymphocyte of the guinea pig. Evidence for thymic origin. J Exp Med. 1972 May 1;135(5):1037–1048. doi: 10.1084/jem.135.5.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rosenthal A. S., Davie J. M., Rosenstreich D. L., Blake J. T. Depletion of antibody-forming cells and their precursors from complex lymphoid cell populations. J Immunol. 1972 Jan;108(1):279–281. [PubMed] [Google Scholar]
  18. Sonozaki H., Cohen S. The macrophage disappearance reaction. II. Mediation by lymphocytes which lack complement receptors. Cell Immunol. 1972 Apr;3(4):644–652. doi: 10.1016/0008-8749(72)90126-8. [DOI] [PubMed] [Google Scholar]
  19. Wilson A. B., Coombs R. R. Rosette-formation between guinea pig lymphoid cells and rabbit erythrocytes--a possible T-cell marker. Int Arch Allergy Appl Immunol. 1973;44(4):544–552. doi: 10.1159/000230959. [DOI] [PubMed] [Google Scholar]
  20. Wood B. T., Thompson S. H., Goldstein G. Fluorescent antibody staining. 3. Preparation of fluorescein-isothiocyanate-labeled antibodies. J Immunol. 1965 Aug;95(2):225–229. [PubMed] [Google Scholar]
  21. Yoshida T. O., Andersson B. Evidence for a receptor recognizing antigen complexed immunoglobulin on the surface of activated mouse thymus lymphocytes. Scand J Immunol. 1972;1(4):401–408. doi: 10.1111/j.1365-3083.1972.tb03306.x. [DOI] [PubMed] [Google Scholar]
  22. van Boxel J. A., Hardin J. A., Green I., Paul W. E. Multiple heavy-chain determinants on individual B lymphocytes in the peripheral blood of patients with Sjögren's syndrome. N Engl J Med. 1973 Oct 18;289(16):823–827. doi: 10.1056/NEJM197310182891602. [DOI] [PubMed] [Google Scholar]

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