Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1974 Apr 1;139(4):969–982. doi: 10.1084/jem.139.4.969

EFFECT OF B-CELL MITOGENS ON LYMPHOCYTE SUBPOPULATIONS POSSESSING C'3 AND Fc RECEPTORS

Göran Möller 1
PMCID: PMC2139565  PMID: 4150198

Abstract

By the use of a rosette method allowing the detection at the cellular level of lymphocytes simultaneously binding Fc- and C'3-sensitized red cells it was found that about 70% of the rosette-forming cells from spleens of nude and normal mice possessed receptors for both Fc and C'3, whereas 30% only had Fc receptors. Very few, if any, lymphocytes possessed only C'3 receptors. The B-cell mitogens, purified-protein derivative of tuberculin (PPD), lipopolysaccharide from Escherichia coli (LPS), and pneumococcal polysaccharide type SIII (SIII), induced marked changes of these receptor-bearing lymphocytes. PPD caused a rapid loss of cells capable of binding C'3 and a concomitant increase of only Fc-binding cells, which was detected after only 24 h. LPS and SIII induced analogous changes, but they were not detected until 48 h and were not complete until after 72 h. It is suggested that immature lymphocytes possess both Fc and C'3 receptors and lose the latter receptor upon differentiation induced by B-cell mitogens PPD. and LPS would affect different populations of B cells, PPD-activating cells that have already reached a higher differentiation stage, whereas LPS and SIII would activate more immature B cells.

Full Text

The Full Text of this article is available as a PDF (929.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Basten A., Miller J. F., Sprent J., Pye J. A receptor for antibody on B lymphocytes. I. Method of detection and functional significance. J Exp Med. 1972 Mar 1;135(3):610–626. doi: 10.1084/jem.135.3.610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bianco C., Patrick R., Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970 Oct 1;132(4):702–720. doi: 10.1084/jem.132.4.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Britton S., Perlmann H., Perlmann P. Thymus-dependent and thymus-independent effector functions of mouse lymphoid cells. Comparison of cytotoxicity and primary antibody formation in vitro. Cell Immunol. 1973 Sep;8(3):420–434. doi: 10.1016/0008-8749(73)90133-0. [DOI] [PubMed] [Google Scholar]
  4. Coutinho A., Möller G., Anderson J., Bullock W. W. In vitro activation of mouse lymphocytes in serum-free medium: effect of T and B cell mitogens on proliferation and antibody synthesis. Eur J Immunol. 1973 May;3(5):299–306. doi: 10.1002/eji.1830030509. [DOI] [PubMed] [Google Scholar]
  5. Coutinho A., Möller G. In vitro induction of specific immune responses in the absence of serum: requirement for nonspecific T or B cell mitogens. Eur J Immunol. 1973 Sep;3(9):531–537. doi: 10.1002/eji.1830030902. [DOI] [PubMed] [Google Scholar]
  6. Dennert G., Lennox E. Cell interactions in humoral and cell-mediated immunity. Nat New Biol. 1972 Jul 26;238(82):114–116. doi: 10.1038/newbio238114a0. [DOI] [PubMed] [Google Scholar]
  7. Eden A., Bianco C., Nussenzweig Mechanism of binding of soluble immune complexes to lymphocytes. Cell Immunol. 1973 Jun;7(3):459–473. doi: 10.1016/0008-8749(73)90210-4. [DOI] [PubMed] [Google Scholar]
  8. Forman J., Möller G. The effector cell in antibody-induced cell mediated immunity. Transplant Rev. 1973;17(0):108–149. doi: 10.1111/j.1600-065x.1973.tb00125.x. [DOI] [PubMed] [Google Scholar]
  9. Greenberg A. H., Hudson L., Shen L., Roitt I. M. Antibody-dependent cell-mediated cytotoxicity due to a "null" lymphoid cell. Nat New Biol. 1973 Mar 28;242(117):111–113. doi: 10.1038/newbio242111a0. [DOI] [PubMed] [Google Scholar]
  10. Lundgren G., Collste L., Möller G. Cytotoxicity of human lymphocytes: antagonism between inducing processes. Nature. 1968 Oct 19;220(5164):289–291. doi: 10.1038/220289a0. [DOI] [PubMed] [Google Scholar]
  11. MacLennan I. C. Antibody in the induction and inhibition of lymphocyte cytotoxicity. Transplant Rev. 1972;13:67–90. doi: 10.1111/j.1600-065x.1972.tb00060.x. [DOI] [PubMed] [Google Scholar]
  12. MacLennan I. C. Competition for receptors for immunoglobulin on cytotoxic lymphocytes. Clin Exp Immunol. 1972 Feb;10(2):275–283. [PMC free article] [PubMed] [Google Scholar]
  13. MacLennan I. C., Harding B. Failure of certain cytotoxic lymphocytes to respond mitotically to phytohaemagglutinin. Nature. 1970 Sep 19;227(5264):1246–1248. doi: 10.1038/2271246a0. [DOI] [PubMed] [Google Scholar]
  14. MacLennan I. C., Loewi G. Effect of specific antibody to target cells on their specific and non-specific interactions with lymphocytes. Nature. 1968 Sep 7;219(5158):1069–1070. doi: 10.1038/2191069a0. [DOI] [PubMed] [Google Scholar]
  15. Miller G. W., Saluk P. H., Nussenzweig V. Complement-dependent release of immune complexes from the lymphocyte membrane. J Exp Med. 1973 Sep 1;138(3):495–507. doi: 10.1084/jem.138.3.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mishell R. I., Dutton R. W. Immunization of normal mouse spleen cell suspensions in vitro. Science. 1966 Aug 26;153(3739):1004–1006. doi: 10.1126/science.153.3739.1004. [DOI] [PubMed] [Google Scholar]
  17. Möller G. DEMONSTRATION OF MOUSE ISOANTIGENS AT THE CELLULAR LEVEL BY THE FLUORESCENT ANTIBODY TECHNIQUE. J Exp Med. 1961 Sep 30;114(4):415–434. doi: 10.1084/jem.114.4.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Perlmann P., Holm G. Cytotoxic effects of lymphoid cells in vitro. Adv Immunol. 1969;11:117–193. doi: 10.1016/s0065-2776(08)60479-4. [DOI] [PubMed] [Google Scholar]
  19. Perlmann P., Perlmann H. Contactual lysis of antibody-coated chicken erythrocytes by purified lymphocytes. Cell Immunol. 1970 Sep;1(3):300–315. doi: 10.1016/0008-8749(70)90051-1. [DOI] [PubMed] [Google Scholar]
  20. Perlmann P., Perlmann H., Wigzell H. Lymphocyte mediated cytotoxicity in vitro. Induction and inhibition by humoral antibody and nature of effector cells. Transplant Rev. 1972;13:91–114. doi: 10.1111/j.1600-065x.1972.tb00061.x. [DOI] [PubMed] [Google Scholar]
  21. Van Boxel J. A., Stobo J. D., Paul W. E., Green I. Antibody-dependent lymphoid cell-mediated cytotoxicity: no requirement for thymus-derived lymphocytes. Science. 1972 Jan 14;175(4018):194–196. doi: 10.1126/science.175.4018.194. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES