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. 1974 Apr 1;139(4):925–942. doi: 10.1084/jem.139.4.925

COMMON CELL SURFACE ANTIGEN ASSOCIATED WITH MAMMALIAN C-TYPE RNA VIRUSES

CELL MEMBRANE-BOUND gs ANTIGEN

Takashi Yoshiki 1, Robert C Mellors 1, William D Hardy Jr 1, Erwin Fleissner 1
PMCID: PMC2139571  PMID: 4131513

Abstract

The indirect membrane immunofluorescence test and the absorption analysis of rabbit anti-FeLV, rabbit anti-FeLVp 30, and rabbit anti-MuLVp 30 antisera yielded the following conclusions. An antigen shared by mammalian (murine and feline) C-type RNA leukemia and sarcoma viruses was detected on the surface of cells infected or transformed by C-type viruses. The antigen was characterized as membrane-bound gs antigen bearing two determinants, membrane-bound gs-1, intraspecies-specific antigenic determinant, and membrane-bound gs-3, interspecies-specific antigenic determinant. Membrane-bound gs antigen was located on the cell surface, frequently near the site of virus budding but not on the envelope of murine C-type RNA virus.

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Selected References

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  1. Aaronson S. A., Parks W. P., Scolnick E. M., Todaro G. J. Antibody to the RNA-dependent DNA polymerase of mammalian C-type RNA tumor viruses. Proc Natl Acad Sci U S A. 1971 May;68(5):920–924. doi: 10.1073/pnas.68.5.920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aoki T., Boyse E. A., Old L. J. Occurrence of natural antibody to the G (gross) leukemia antigen in mice. Cancer Res. 1966 Jul;26(7):1415–1419. [PubMed] [Google Scholar]
  3. Aoki T., Boyse E. A., Old L. J. Wild-type Gross leukemia virus. I. Soluble antigen (GSA) in the plasma and tissues of infected mice. J Natl Cancer Inst. 1968 Jul;41(1):89–96. [PubMed] [Google Scholar]
  4. Geering G., Aoki T., Old L. J. Shared viral antigen of mammalian leukaemia viruses. Nature. 1970 Apr 18;226(5242):265–266. doi: 10.1038/226265a0. [DOI] [PubMed] [Google Scholar]
  5. Geering G., Hardy W. D., Jr, Old L. J., de Harven E., Brodey R. S. Shared group-specific antigen of murine and feline leukemia viruses. Virology. 1968 Dec;36(4):678–680. doi: 10.1016/0042-6822(68)90199-2. [DOI] [PubMed] [Google Scholar]
  6. Gilden R. V., Oroszlan S. Group-specific antigens of RNA tumor viruses as markers for subinfectious expression of the RNA virus genome. Proc Natl Acad Sci U S A. 1972 Apr;69(4):1021–1025. doi: 10.1073/pnas.69.4.1021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gilden R. V., Oroszlan S., Huebner R. J. Coexistence of intraspecies and interspecies specific antigenic determinants on the major structural polypeptide of mammalian C-type viruses. Nat New Biol. 1971 May 26;231(21):107–108. doi: 10.1038/newbio231107a0. [DOI] [PubMed] [Google Scholar]
  8. Girardi A., Hampar B., Hsu K. C., Oroszlan S., Hornberger E., Kelloff G., Gilden R. V. Intracellular localization of mammalian type C virus species-specific (gs-1) and interspecies-specific (gs-3) antigenic determinants with the indirect immunoperoxidase technique and light microscopy. J Immunol. 1973 Jul;111(1):152–156. [PubMed] [Google Scholar]
  9. Gregoriades A., Old L. J. Isolation and some characteristics of a group-specific antigen of the murine leukemia viruses. Virology. 1969 Feb;37(2):189–202. doi: 10.1016/0042-6822(69)90198-6. [DOI] [PubMed] [Google Scholar]
  10. Hardy W. D., Jr Immunodiffusion studies of feline leukemia and sarcoma. J Am Vet Med Assoc. 1971 Mar 15;158(6 Suppl):1060+–1060+. [PubMed] [Google Scholar]
  11. Hilgers J., Nowinski R. C., Geering G., Hardy W. Detection of avian and mammalian oncogenic RNA viruses (oncornaviruses) by immunofluorescence. Cancer Res. 1972 Jan;32(1):98–106. [PubMed] [Google Scholar]
  12. Miyamoto K., Gilden R. V. Electron microscopic studies of tumor viruses. I. Entry of murine leukemia virus into mouse embryo fibroblasts. J Virol. 1971 Mar;7(3):395–406. doi: 10.1128/jvi.7.3.395-406.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nowinski R. C., Fleissner E., Sarkar N. H., Aoki T. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. II. Mammalian leukemia-sarcoma viruses. J Virol. 1972 Feb;9(2):359–366. doi: 10.1128/jvi.9.2.359-366.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Old L. J., Boyse E. A., Stockert E. The G (Gross) leukemia antigen. Cancer Res. 1965 Jul;25(6):813–819. [PubMed] [Google Scholar]
  15. Oroszlan S., Bova D., Huebner R. J., Gilden R. V. Major group-specific protein of rat type C viruses. J Virol. 1972 Oct;10(4):746–750. doi: 10.1128/jvi.10.4.746-750.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Oroszlan S., Foreman C., Kelloff G., Gilden R. V. The group-specific antigen and other structural proteins of hamster and mouse C-type viruses. Virology. 1971 Mar;43(3):665–674. doi: 10.1016/0042-6822(71)90290-x. [DOI] [PubMed] [Google Scholar]
  17. Oroszlan S., Huebner R. J., Gilden R. V. Species-specific and interspecific antigenic determinants associated with the structural protein of feline C-type virus. Proc Natl Acad Sci U S A. 1971 May;68(5):901–904. doi: 10.1073/pnas.68.5.901. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Parks W. P., Scolnick E. M. Radioimmunoassay of mammalian type-C viral proteins: interspecies antigenic reactivities of the major internal polypeptide. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1766–1770. doi: 10.1073/pnas.69.7.1766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Raff M. C., De Petris S. Movement of lymphocyte surface antigens and receptors: the fluid nature of the lymphocyte plasma membrane and its immunological significance. Fed Proc. 1973 Jan;32(1):48–54. [PubMed] [Google Scholar]
  20. Rowe W. P., Pincus T. Quantitative studies of naturally occurring murine leukemia virus infection of AKR mice. J Exp Med. 1972 Feb 1;135(2):429–436. doi: 10.1084/jem.135.2.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. STUECK B., OLD L. J., BOYSE E. A. OCCURRENCE OF SOLUBLE ANTIGEN IN THE PLASMA OF MICE WITH VIRUS-INDUCED LEUKEMIA. Proc Natl Acad Sci U S A. 1964 Oct;52:950–958. doi: 10.1073/pnas.52.4.950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sarma P. S., Log T. Subgroup classification of feline leukemia and sarcoma viruses by viral interference and neutralization tests. Virology. 1973 Jul;54(1):160–169. doi: 10.1016/0042-6822(73)90125-6. [DOI] [PubMed] [Google Scholar]
  23. Scolnick E. M., Parks W. P., Todaro G. J., Aaronson S. A. Immunological characterization of primate C-type virus reverse transcriptases. Nat New Biol. 1972 Jan 12;235(54):35–40. doi: 10.1038/newbio235035a0. [DOI] [PubMed] [Google Scholar]
  24. Shirai T., Yoshiki T., Mellors R. C. Thymus dependence of cells in peripheral lymphoid tissues and in the circulation sensitive to natural thymocytotoxic autoantibody in NZB mice. J Immunol. 1972 Jul;109(1):32–37. [PubMed] [Google Scholar]
  25. Strand M., August J. T. Structural proteins of mammalian oncogenic RNA viruses: multiple antigenic determinants of the major internal protein and envelope glycoprotein. J Virol. 1974 Jan;13(1):171–180. doi: 10.1128/jvi.13.1.171-180.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Strand M., August J. T. Structural proteins of oncogenic ribonucleic acid viruses. Interspec II, a new interspecies antigen. J Biol Chem. 1973 Aug 25;248(16):5627–5633. [PubMed] [Google Scholar]
  27. Yoshiki T., Mellors R. C., Hardy W. D., Jr Common cell-surface antigen associated with murine and feline C-type RNA leukemia viruses. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1878–1882. doi: 10.1073/pnas.70.6.1878. [DOI] [PMC free article] [PubMed] [Google Scholar]

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