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. 1974 Aug 1;140(2):383–395. doi: 10.1084/jem.140.2.383

ALLOANTISERUM-INDUCED INHIBITION OF MIGRATION INHIBITION FACTOR PRODUCTION IN IMMUNE RESPONSE GENE-CONTROLLED IMMUNE SYSTEMS

Shlomo Z Ben-Sasson 1, Ethan Shevach 1, Ira Green 1, William E Paul 1
PMCID: PMC2139581  PMID: 4603011

Abstract

We have previously demonstrated that alloantisera prepared by reciprocal immunization of strain 2 and strain 13 guinea pigs specifically block stimulation of in vitro DNA synthesis in genetically controlled systems. In order to determine whether this blockade extends to other T-lymphocyte functions, we examined the effect of alloantisera on the production of migration inhibition factor (MIF). (2 x 13)F1 guinea pigs were immunized with a DNP derivative of the copolymer of L-glutamic acid and L-lysine (DNP-GL) and with DNP guinea pig albumin (GPA). The response to the former is controlled by a 2-linked Ir gene while that to the latter is mainly controlled by a 13-linked Ir gene. MIF production was assayed by an indirect procedure in which the migrating cell population lacked the histocompatibility antigen against which the alloantiserum was directed. Our results showed that anti-2 serum blocked MIF production by F1 cells in response to DNP-GL but not DNP-GPA while anti-13 serum had the opposite effect. These experiments show that expression of a second major T-cell function is specifically blocked by alloantisera and suggest that Ir-gene products may act as antigen recognition substances on more than one type of T cell.

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Selected References

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  1. Bach F. H., Segall M., Zier K. S., Sondel P. M., Alter B. J., Bach M. L. Cell mediated immunity: separation of cells involved in recognitive and destructive phases. Science. 1973 Apr 27;180(4084):403–406. doi: 10.1126/science.180.4084.403. [DOI] [PubMed] [Google Scholar]
  2. Bach M. L., Huang S. W., Hong R., Poulik M. D. Beta 2-microglobulin: association with lymphocyte receptors. Science. 1973 Dec 28;182(4119):1350–1352. doi: 10.1126/science.182.4119.1350. [DOI] [PubMed] [Google Scholar]
  3. Basten A., Miller J. F., Warner N. L., Pye J. Specific inactivation of thymus-derived (T) and non-thymus-derived (B) lymphocytes by 125I-labelled antigen. Nat New Biol. 1971 May 26;231(21):104–106. doi: 10.1038/newbio231104a0. [DOI] [PubMed] [Google Scholar]
  4. Benacerraf B., McDevitt H. O. Histocompatibility-linked immune response genes. Science. 1972 Jan 21;175(4019):273–279. doi: 10.1126/science.175.4019.273. [DOI] [PubMed] [Google Scholar]
  5. Bluestein H. G., Ellman L., Green I., Benacerraf B. Specific immune response genes of the guinea pig. 3. Linkage of the GA and GT immune response genes to histocompatibility genotypes in inbred guinea pigs. J Exp Med. 1971 Dec 1;134(6):1529–1537. doi: 10.1084/jem.134.6.1529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cantor H., Asofsky R. Synergy among lymphoid cells mediating the graft-versus-host response. II. Synergy in graft-versus-host reactions produced by Balb-c lymphoid cells of differing anatomic origin. J Exp Med. 1970 Feb;131(2):235–246. doi: 10.1084/jem.131.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chaparas S. D., Thor D. E., Godfrey H. P., Baer H., Hedrick S. R. Tuberculin-active carbohydrate that induces inhibition of macrophage migration but not lymphocyte transformation. Science. 1970 Nov 6;170(3958):637–639. doi: 10.1126/science.170.3958.637. [DOI] [PubMed] [Google Scholar]
  8. Chapuis B., Brunner K. T. Cell mediated immune reactions "in vitro". Reactivity of lymphocytes from animals sensitized to chicken erythrocytes, tuberculin or transplantation antigens. Int Arch Allergy Appl Immunol. 1971;40(3):321–339. [PubMed] [Google Scholar]
  9. David J. R., Schlossman S. F. Immunochemical studies on the specificity of cellular hypersensitivity. The in vitro inhibition of peritoneal exudate cell migration by cehmically defined antigens. J Exp Med. 1968 Dec 1;128(6):1451–1459. doi: 10.1084/jem.128.6.1451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ellman L., Green I., Martin W. J., Benacerraf B. Linkage between the poly-L-lysine gene and the locus controlling the major histocompatibility antigens in strain 2 guinea pigs. Proc Natl Acad Sci U S A. 1970 Jun;66(2):322–328. doi: 10.1073/pnas.66.2.322. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Green I., Benacerraf B. Genetic control of immune responsiveness to limiting doses of proteins and hapten protein conjugates in guinea pigs. J Immunol. 1971 Aug;107(2):374–381. [PubMed] [Google Scholar]
  12. Green I., Paul W. E., Benacerraf B. Histocompatibility-linked genetic control of the immune response to hapten guinea pig albumin conjugates in inbred guinea pigs. J Immunol. 1972 Sep;109(3):457–463. [PubMed] [Google Scholar]
  13. KANTOR F. S., OJEDA A., BENCARERRAF B. Studies on artifical antigens. I. Antigenicity of DNP-polylysine and DNP copolymer of lysine and glutamic acid in guinea pigs. J Exp Med. 1963 Jan 1;117:55–69. doi: 10.1084/jem.117.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Katz D. H., Benacerraf B. The regulatory influence of activated T cells on B cell responses to antigen. Adv Immunol. 1972;15:1–94. doi: 10.1016/s0065-2776(08)60683-5. [DOI] [PubMed] [Google Scholar]
  15. Levine B. B., Stember R. H., Fotino M. Ragweed hay fever: genetic control and linkage to HL-A haplotypes. Science. 1972 Dec 15;178(4066):1201–1203. doi: 10.1126/science.178.4066.1201. [DOI] [PubMed] [Google Scholar]
  16. McDevitt H. O., Benacerraf B. Genetic control of specific immune responses. Adv Immunol. 1969;11:31–74. doi: 10.1016/s0065-2776(08)60477-0. [DOI] [PubMed] [Google Scholar]
  17. Rocklin R. E. Production of migration inhibitory factor by non-dividing lymphocytes. J Immunol. 1973 Mar;110(3):674–678. [PubMed] [Google Scholar]
  18. Rosenstreich D. L., Blake J. T., Rosenthal A. S. The peritoneal exudate lymphocyte. I. Differences in antigen responsiveness between peritoneal exudate and lymph node lymphocytes from immunized guinea pigs. J Exp Med. 1971 Nov 1;134(5):1170–1186. doi: 10.1084/jem.134.5.1170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shevach E. M., Paul W. E., Green I. Histocompatibility-linked immune response gene function in guinea pigs. Specific inhibition of antigen-induced lymphocyte proliferation by alloantisera. J Exp Med. 1972 Nov 1;136(5):1207–1221. doi: 10.1084/jem.136.5.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shevach E. M., Rosenstreich D. L., Green I. The distribution of histocompatibility antigens on T and B cells in the guinea pig. Transplantation. 1973 Aug;16(2):126–133. doi: 10.1097/00007890-197308000-00008. [DOI] [PubMed] [Google Scholar]
  21. Spitler L. E., Von Muller C. M., Fudenberg H. H., Eylar E. H. Experimental allergic encephalitis. Dissociation of cellular immunity to brain protein and disease production. J Exp Med. 1972 Jul 1;136(1):156–174. doi: 10.1084/jem.136.1.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Spitler L., Benjamini E., Young J. D., Kaplan H., Fudenberg H. H. Studies on the immune response to a characterized antigenic determinant of the tobacco mosaic virus protein. J Exp Med. 1970 Jan 1;131(1):133–148. doi: 10.1084/jem.131.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stobo J. D., Paul W. E., Henney C. S. Functional heterogeneity of murine lymphoid cells. IV. Allogeneic mixed lymphocyte reactivity and cytolytic activity as functions of distinct T cell subsets. J Immunol. 1973 Mar;110(3):652–660. [PubMed] [Google Scholar]
  24. Valdimarsson H., Higgs J. M., Wells R. S., Yamamura M., Hobbs J. R., Holt P. J. Immune abnormalities associated with chronic mucocutaneous candidiasis. Cell Immunol. 1973 Mar;6(3):348–361. doi: 10.1016/0008-8749(73)90035-x. [DOI] [PubMed] [Google Scholar]
  25. Wernet P., Kunkel H. G. Antibodies to a specific surface antigen of T cells in human sera inhibiting mixed leukocyte culture reactions. J Exp Med. 1973 Oct 1;138(4):1021–1026. doi: 10.1084/jem.138.4.1021. [DOI] [PMC free article] [PubMed] [Google Scholar]

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