Abstract
Five inbred mouse strains which represent high and low responders to the random synthetic polypeptide poly(LTyr,LGlu)-polyDLAla--polyLLys, designated (T, G)-A--L, to which the immune response is controlled by an H-2-linked gene, were immunized with three ordered tetrapeptides composed of tyrosine and glutamic acid attached either to multichain poly-DL-alanine or to polyproline. Only one of the three antigenic determinants, namely tyrosyl-tyrosyl-glytamyl-glutamic acid (T-T-G-G), resembled the random peptide (T, G) in the pattern of immune responses elicited against it, and in the cross-reactivity of the specific antibodies with (T, G)-A--L. The immune response pattern to the other two ordered tetrapeptides, T-G-T-G and G-T-T-G, was different from that obtained with (T, G)-A--L, and no cross-reactivity was detected between the antibodies provoked with these peptides and (T, G)-A--L. Thus, it is suggested that T-T-G-G is a major determinant in the random (T, G)-A--L.
Full Text
The Full Text of this article is available as a PDF (375.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Benacerraf B., McDevitt H. O. Histocompatibility-linked immune response genes. Science. 1972 Jan 21;175(4019):273–279. doi: 10.1126/science.175.4019.273. [DOI] [PubMed] [Google Scholar]
- Jaton J. C., Sela M. Role of optical configuration in the immunogenicity and specificity of synthetic antigens derived from multichain polyproline. J Biol Chem. 1968 Nov 10;243(21):5616–5626. [PubMed] [Google Scholar]
- McDevitt H. O., Chinitz A. Genetic control of the antibody response: relationship between immune response and histocompatibility (H-2) type. Science. 1969 Mar 14;163(3872):1207–1208. doi: 10.1126/science.163.3872.1207. [DOI] [PubMed] [Google Scholar]
- McDevitt H. O., Sela M. Genetic control of the antibody response. I. Demonstration of determinant-specific differences in response to synthetic polypeptide antigens in two strains of inbred mice. J Exp Med. 1965 Sep 1;122(3):517–531. doi: 10.1084/jem.122.3.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McDevitt H. O., Sela M. Genetic control of the antibody response. II. Further analysis of the specificity of determinant-specific control, and genetic analysis of the response to (H,G)-A--L in CBA and C57 mice. J Exp Med. 1967 Nov 1;126(5):969–978. doi: 10.1084/jem.126.5.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McDevitt H. O., Tyan M. L. Genetic control of the antibody response in inbred mice. Transfer of response by spleen cells and linkage to the major histocompatibility (H-2) locus. J Exp Med. 1968 Jul 1;128(1):1–11. [PMC free article] [PubMed] [Google Scholar]
- Mozes E., Maron E., Arnon R., Sela M. [Strain-dependent differences in the specificity of antibody responses toward lysozyme]. J Immunol. 1971 Mar;106(3):862–864. [PubMed] [Google Scholar]
- Mozes E., McDevitt H. O., Jaton J. C., Sela M. The genetic control of antibody specificity. J Exp Med. 1969 Dec 1;130(6):1263–1278. doi: 10.1084/jem.130.6.1263. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mozes E., McDevitt H. O., Jaton J. C., Sela M. The nature of the antigenic determinant in a genetic control of the antibody response. J Exp Med. 1969 Sep 1;130(3):493–504. doi: 10.1084/jem.130.3.493. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mozes E., Shearer G. M. Genetic control of immune responses. Curr Top Microbiol Immunol. 1972;59:167–200. doi: 10.1007/978-3-642-65444-2_6. [DOI] [PubMed] [Google Scholar]
- Ramachandran J., Berger A., Katchalski E. Synthesis and physicochemical properties in aqueous solution of the sequential polypeptide poly(Tyr-Ala-Glu). Biopolymers. 1971 Oct;10(10):1829–1851. doi: 10.1002/bip.360101007. [DOI] [PubMed] [Google Scholar]
- SELA M., FUCHS S., ARNON R. Studies on the chemical basis of the antigenicity of proteins. 5. Synthesis, characterization and immunogenicity of some multichain and linear polypeptides containing tyrosine. Biochem J. 1962 Oct;85:223–235. doi: 10.1042/bj0850223. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schechter B., Schechter I., Ramachandran J., Conway-Jacobs A., Sela M., Benjamini E., Shimizu M. Synthetic antigens with sequential and conformation-dependent determinants containing the same L-tyrosyl-L-alanyl-L-glutamyl sequence. Eur J Biochem. 1971 Jun 11;20(3):309–320. doi: 10.1111/j.1432-1033.1971.tb01395.x. [DOI] [PubMed] [Google Scholar]
- Sela M. Immunological studies with synthetic polypeptides. Adv Immunol. 1966;5:29–129. doi: 10.1016/s0065-2776(08)60272-2. [DOI] [PubMed] [Google Scholar]