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. 1974 Sep 1;140(3):660–672. doi: 10.1084/jem.140.3.660

SPECIFIC POSITIVE SELECTION OF LYMPHOCYTES REACTIVE TO STRONG HISTOCOMPATIBILITY ANTIGENS

Jonathan C Howard 1, Darcy B Wilson 1
PMCID: PMC2139612  PMID: 4137934

Abstract

Selected populations of thymus-derived (T) rat lymphocytes having specific immunological reactivity to chosen histocompatibility (H) alloantigens are found among the cellular products of the mixed lymphocyte interaction (MLI). Such specific selection seems to depend on (a) the antigen-induced proliferation of specific H antigen reactive cells (HARC), and (b) the disappearance of nonreactive cells from the cultures. When the surviving cells from this lymphocyte-antigen interaction are transferred into thymectomized, X-irradiated, marrow-reconstituted syngeneic recipients (B rats) which lack detectable T-lymphocyte functions, the lymphocyte populations subsequently recovered from the hosts possess the capacity to react in the MLI and in the graft-vs.-host (GVH) reaction, and the reactions have specificity for the original priming alloantigens. In addition, these findings identify the cell that reacts in the MLI with the GVH reactive cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson L. C., Häyry P. Specific priming of mouse thymus-dependent lymphocytes to allogeneic cells in vitro. Eur J Immunol. 1973 Sep;3(9):595–599. doi: 10.1002/eji.1830030913. [DOI] [PubMed] [Google Scholar]
  2. Bach F. H., Bock H., Graupner K., Day E., Klostermann H. Cell kinetic studies in mixed leukocyte cultures: an in vitro model of homograft reactivity. Proc Natl Acad Sci U S A. 1969 Feb;62(2):377–384. doi: 10.1073/pnas.62.2.377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brent L., Medawar P. Quantitative studies on tissue transplantation immunity. VII. The normal lymphocyte transfer reaction. Proc R Soc Lond B Biol Sci. 1966 Sep 13;165(1000):281–307. doi: 10.1098/rspb.1966.0069. [DOI] [PubMed] [Google Scholar]
  4. Ford W. L., Atkins R. C. Specific unresponsiveness of recirculating lymphocytes ater exposure to histocompatibility antigen in F 1 hybrid rats. Nat New Biol. 1971 Dec 8;234(49):178–180. doi: 10.1038/newbio234178a0. [DOI] [PubMed] [Google Scholar]
  5. Ford W. L., Atkins R. C. The proportion of lymphocytes capable of recognizing strong transplantation antigens in vivo. Adv Exp Med Biol. 1973;29(0):255–262. doi: 10.1007/978-1-4615-9017-0_37. [DOI] [PubMed] [Google Scholar]
  6. Ford W. L., Burr W., Simonsen M. A lymph node weight assay for the graft-versus-host activity of rat lymphoid cells. Transplantation. 1970 Sep;10(3):258–266. doi: 10.1097/00007890-197009000-00007. [DOI] [PubMed] [Google Scholar]
  7. Howard J. C. On the relationship between initiator and effector cells in the response to major tansplantation antigens. Transplant Proc. 1973 Dec;5(4):1451–1456. [PubMed] [Google Scholar]
  8. Howard J. C. The life-span and recirculation of marrow-derived small lymphocytes from the rat thoracic duct. J Exp Med. 1972 Feb 1;135(2):185–199. doi: 10.1084/jem.135.2.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnston J. M., Wilson D. B. Origin of immunoreactive lymphocytes in rats. Cell Immunol. 1970 Oct;1(4):430–444. doi: 10.1016/0008-8749(70)90019-5. [DOI] [PubMed] [Google Scholar]
  10. Nisbet N. W., Simonsen M., Zaleski M. The frequency of antigen-sensitive cells in tissue transplantation. A commentary on clonal selection. J Exp Med. 1969 Mar 1;129(3):459–467. doi: 10.1084/jem.129.3.459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Palm J. Immunogenetic analysis of Ag-B histocompatibility antigens in rats. Transplantation. 1971 Feb;11(2):175–183. doi: 10.1097/00007890-197102000-00011. [DOI] [PubMed] [Google Scholar]
  12. Scott D. W., Howard J. C. Collaboration between thymus-derived and marrow-derived thoracic duct lymphocytes in the hemolysin response of the rat. Cell Immunol. 1972 Mar;3(3):430–441. doi: 10.1016/0008-8749(72)90248-1. [DOI] [PubMed] [Google Scholar]
  13. Wagner H., Feldmann M. Cell-mediated immune response in vitro. I. A new in vitro system for the generation of cell-mediated cytotoxic activity. Cell Immunol. 1972 Mar;3(3):405–420. doi: 10.1016/0008-8749(72)90246-8. [DOI] [PubMed] [Google Scholar]
  14. Wilson D. B., Blyth JL NOWELL P. C. Quantitative studies on the mixed lymphocyte interaction in rats. 3. Kinetics of the response. J Exp Med. 1968 Nov 1;128(5):1157–1181. doi: 10.1084/jem.128.5.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wilson D. B., Nowell P. C. Quantitative studies on the mixed lymphocyte interaction in rats. V. Tempo and specificity of the proliferative response and the number of reactive cells from immunized donors. J Exp Med. 1971 Mar 1;133(3):442–453. doi: 10.1084/jem.133.3.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wilson D. B. Quantitative studies on the mixed lymphocyte interaction in rats. I. Conditions and parameters of response. J Exp Med. 1967 Oct 1;126(4):625–654. doi: 10.1084/jem.126.4.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Zoschke D. C., Bach F. H. Specificity of allogeneic cell recognition by human lymphocytes in vitro. Science. 1971 Jun 25;172(3990):1350–1352. doi: 10.1126/science.172.3990.1350. [DOI] [PubMed] [Google Scholar]

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