Abstract
Bone marrow cells from CBA T6T6 mice and testicular teratocarcinoma cells from 129 SvSl mice were transferred into blastocysts from random-bred Swiss albino mice. The blastocysts were allowed to develop in foster mothers and the adults resulting from these blastocysts were studied for evidence of an effect of the transferred cells. A total of 137 adults resulted from the experiments, and one of the mice that had received teratocarcinoma cells in the blastocyst stage showed several thin stripes of agouti hair. All the adult animals received grafts of skin from animals identical to those supplying the cells. In all cases the animals that resulted from blastocysts into which cells had been transferred maintained skin grafts for a significantly longer period than controls. In a number of cases the graft developed agouti hair and in two cases the graft was maintained for approximately 2 mo. These experiments indicate that the transferred cells were able to establish small colonies in the embryos and that some of these cells persisted into the adult.
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Selected References
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- BRINSTER R. L. A METHOD FOR IN VITRO CULTIVATION OF MOUSE OVA FROM TWO-CELL TO BLASTOCYST. Exp Cell Res. 1963 Oct;32:205–208. doi: 10.1016/0014-4827(63)90093-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gardner R. L., Johnson M. H. An investigation of inner cell mass and trophoblast tissues following their isolation from the mouse blastocyst. J Embryol Exp Morphol. 1972 Oct;28(2):279–312. [PubMed] [Google Scholar]
- Gardner R. L. Mouse chimeras obtained by the injection of cells into the blastocyst. Nature. 1968 Nov 9;220(5167):596–597. doi: 10.1038/220596a0. [DOI] [PubMed] [Google Scholar]
- Gasser D. L., Silvers W. K. Genetics and immunology of sex-linked antigens. Adv Immunol. 1972;15:215–247. doi: 10.1016/s0065-2776(08)60686-0. [DOI] [PubMed] [Google Scholar]
- Haskill J. S., Moore M. A. Two dimensional cell separation: comparison of embryonic and adult haemopoietic stem cells. Nature. 1970 May 30;226(5248):853–854. doi: 10.1038/226853a0. [DOI] [PubMed] [Google Scholar]
- Hayry P., Virolainen M., Defendi V. Method for chromosome preparation from mouse peripheral lymphocytes. Proc Soc Exp Biol Med. 1970 Feb;133(2):637–641. doi: 10.3181/00379727-133-34534. [DOI] [PubMed] [Google Scholar]
- LITCHFIELD J. T., Jr A method for rapid graphic solution of time-per cent effect curves. J Pharmacol Exp Ther. 1949 Dec;97(4):399-408, 3 tab. [PubMed] [Google Scholar]
- LITCHFIELD J. T., Jr, WILCOXON F. A simplified method of evaluating dose-effect experiments. J Pharmacol Exp Ther. 1949 Jun;96(2):99–113. [PubMed] [Google Scholar]
- Moustafa L. A., Brinster R. L. Induced chimaerism by transplanting embryonic cells into mouse blastocysts. J Exp Zool. 1972 Aug;181(2):193–201. doi: 10.1002/jez.1401810206. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moustafa L. A., Brinster R. L. The fate of transplanted cells in mouse blastocysts in vitro. J Exp Zool. 1972 Aug;181(2):181–191. doi: 10.1002/jez.1401810205. [DOI] [PubMed] [Google Scholar]
- Silvers W. K., Billingham R. E. Genetic background and expressivity of histocompatibility genes. Science. 1967 Oct 6;158(3797):118–119. doi: 10.1126/science.158.3797.118. [DOI] [PubMed] [Google Scholar]
- Stevens L. C., Little C. C. Spontaneous Testicular Teratomas in an Inbred Strain of Mice. Proc Natl Acad Sci U S A. 1954 Nov;40(11):1080–1087. doi: 10.1073/pnas.40.11.1080. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stevens L. C. The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Dev Biol. 1970 Mar;21(3):364–382. doi: 10.1016/0012-1606(70)90130-2. [DOI] [PubMed] [Google Scholar]
- TARKOWSKI A. K. Mouse chimaeras developed from fused eggs. Nature. 1961 Jun 3;190:857–860. doi: 10.1038/190857a0. [DOI] [PubMed] [Google Scholar]
- van Bekkum D. W., van Noord M. J., Maat B., Dicke K. A. Attempts at identification of hemopoietic stem cell in mouse. Blood. 1971 Nov;38(5):547–558. [PubMed] [Google Scholar]