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. 1974 May 1;139(5):1070–1076. doi: 10.1084/jem.139.5.1070

EPSTEIN-BARR VIRUS-NEGATIVE HUMAN MALIGNANT T-CELL LINES

Joseph Kaplan 1, Thomas C Shope 1, Ward D Peterson Jr 1
PMCID: PMC2139653  PMID: 4363409

Abstract

Two lymphoblastoid lines, CCRF-CEM and HSB-2, with properties of malignant cells, derived from children with leukemia secondary to lymphosarcoma, have T-cell properties and lack Epstein-Barr virus antigens.

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Selected References

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  1. Adams R. A., Flowers A., Davis B. J. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 1968 Jun;28(6):1121–1125. [PubMed] [Google Scholar]
  2. Adams R. A., Flowers A., Sundeen R., Merk L. P. Chemotherapy and immunotherapy of three human lymphomas serially transplantable in the neonatal Syrian hamster. Cancer. 1972 Feb;29(2):524–533. doi: 10.1002/1097-0142(197202)29:2<524::aid-cncr2820290244>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  3. Adams R. A., Pothier L., Flowers A., Lazarus H., Farber S., Foley G. E. The question of stemlines in human acute leukemia. Comparison of cells isolated in vitro and in vivo from a patient with acute lymphoblastic leukemia. Exp Cell Res. 1970 Sep;62(1):5–10. doi: 10.1016/0014-4827(79)90504-4. [DOI] [PubMed] [Google Scholar]
  4. Adams R. A., Pothier L., Hellerstein E. E., Boileau G. Malignant immunoblastoma: immunoglobulin synthesis and the progression to leukemia in heterotransplanted acute lymphoblastic leukemia, chronic lymphatic leukemia, lymphoma, and infectious mononucleosis. Cancer. 1973 Jun;31(6):1397–1407. doi: 10.1002/1097-0142(197306)31:6<1397::aid-cncr2820310615>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  5. Epstein M. A., Hunt R. D., Rabin H. Pilot experiments with EB virus in owl monkeys (Aotus trivirgatus). I Reticuloproliferative disease in an inoculated animal. Int J Cancer. 1973 Sep 15;12(2):309–318. doi: 10.1002/ijc.2910120202. [DOI] [PubMed] [Google Scholar]
  6. Epstein M. A., Rabin H., Ball G., Rickinson A. B., Jarvis J., Meléndez L. V. Pilot experiments with EB virus in owl monkeys (Aotus trivirgatus). II. EB virus in a cell line from an animal with reticuloproliferative disease. Int J Cancer. 1973 Sep 15;12(2):319–332. doi: 10.1002/ijc.2910120203. [DOI] [PubMed] [Google Scholar]
  7. Gerber P. Activation of Epstein-Barr virus by 5-bromodeoxyuridine in "virus-free" human cells (complement-fixing antigen-immunofluorescence-leukocytes). Proc Natl Acad Sci U S A. 1972 Jan;69(1):83–85. doi: 10.1073/pnas.69.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Henle G., Henle W. Immunofluorescence in cells derived from Burkitt's lymphoma. J Bacteriol. 1966 Mar;91(3):1248–1256. doi: 10.1128/jb.91.3.1248-1256.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hinuma Y., Grace J. T., Jr Cloning of immunoglobulin-producing human leukemic and lymphoma cells in long-term cultures. Proc Soc Exp Biol Med. 1967 Jan;124(1):107–111. doi: 10.3181/00379727-124-31677. [DOI] [PubMed] [Google Scholar]
  10. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jondal M., Klein G. Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes. J Exp Med. 1973 Dec 1;138(6):1365–1378. doi: 10.1084/jem.138.6.1365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kaplan J., Mastrangelo R., Peterson W. D., Jr Childhood lymphoblastic lymphoma, a cancer of thymus-derived lymphocytes. Cancer Res. 1974 Mar;34(3):521–525. [PubMed] [Google Scholar]
  13. Klein E., Klein G., Nadkarni J. S., Nadkarni J. J., Wigzell H., Clifford P. Surgace IgM specificity on cells derived from a Burkitt's lymphoma. Lancet. 1967 Nov 18;2(7525):1068–1070. doi: 10.1016/s0140-6736(67)90340-6. [DOI] [PubMed] [Google Scholar]
  14. Maurer B. A., Wilbert S. M., Imamura T. Incidence of EB virus-containing cells in primary and secondary clones of several Burkitt lymphoma cell lines. Cancer Res. 1970 Dec;30(12):2870–2875. [PubMed] [Google Scholar]
  15. Minowada J., Onuma T., Moore G. E. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Natl Cancer Inst. 1972 Sep;49(3):891–895. [PubMed] [Google Scholar]
  16. Moore G. E. Cultured human lymphocytes. J Surg Oncol. 1972;4(4):320–353. doi: 10.1002/jso.2930040405. [DOI] [PubMed] [Google Scholar]
  17. Nilsson K. High-frequency establishment of human immunoglobulin-producing lymphoblastoid lines from normal and malignant lymphoid tissue and peripheral blood. Int J Cancer. 1971 Nov 15;8(3):432–442. doi: 10.1002/ijc.2910080311. [DOI] [PubMed] [Google Scholar]
  18. Nilsson K., Klein G., Henle W., Henle G. The establishment of lymphoblastoid lines from adult and fetal human lymphoid tissue and its dependence on EBV. Int J Cancer. 1971 Nov 15;8(3):443–450. doi: 10.1002/ijc.2910080312. [DOI] [PubMed] [Google Scholar]
  19. Nilsson K., Pontén J., Philipson L. Development of immunocytes and immunoglobulin production in long-term cultures from normal and malignant human lymph nodes. Int J Cancer. 1968 Mar 15;3(2):183–190. doi: 10.1002/ijc.2910030203. [DOI] [PubMed] [Google Scholar]
  20. Papamichail M., Brown J. C., Holborow E. J. Immunoglobulins on the surface of human lymphocytes. Lancet. 1971 Oct 16;2(7729):850–852. doi: 10.1016/s0140-6736(71)90224-8. [DOI] [PubMed] [Google Scholar]
  21. Pincus S., Bianco C., Nussenzweig V. Increased proportion of complement-receptor lymphocytes in the peripheral blood of patients with chronic lymphocytic leukemia. Blood. 1972 Sep;40(3):303–310. [PubMed] [Google Scholar]
  22. Pope J. H., Horne M. K., Wetters E. J. Significance of a complement-fixing antigen associated with herpes-like virus and detected in the Raji cell line. Nature. 1969 Apr 12;222(5189):186–187. doi: 10.1038/222186a0. [DOI] [PubMed] [Google Scholar]
  23. Reedman B. M., Klein G. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines. Int J Cancer. 1973 May;11(3):499–520. doi: 10.1002/ijc.2910110302. [DOI] [PubMed] [Google Scholar]
  24. Sherr C. J., Uhr J. W. Immunoglobulin synthesis and secretion. VI. Synthesis and intracellular transport of immunoglobulin in nonsecretory lymphoma cells. J Exp Med. 1971 Apr 1;133(4):901–920. doi: 10.1084/jem.133.4.901. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shope T., Dechairo D., Miller G. Malignant lymphoma in cottontop marmosets after inoculation with Epstein-Barr virus. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2487–2491. doi: 10.1073/pnas.70.9.2487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wybran J., Carr M. C., Fudenberg H. H. The human rosette-forming cell as a marker of a population of thymus-derived cells. J Clin Invest. 1972 Oct;51(10):2537–2543. doi: 10.1172/JCI107069. [DOI] [PMC free article] [PubMed] [Google Scholar]

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