Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1974 May 1;139(5):1049–1060. doi: 10.1084/jem.139.5.1049

CYTOTOXIC ACTIVITY OF MOUSE MACROPHAGES STUDIED BY VARIOUS INHIBITORS

Haakon Melsom 1
PMCID: PMC2139660  PMID: 4825241

Abstract

The influence of various inhibitors on the cytolytic potential of mouse macrophages against syngeneic erythrocytes has been investigated in vitro by isotope techniques. Intact macrophage membrane and cell metabolism was essential for full cytotoxic activity. The process was completely blocked by anaerobiosis and cold. ATP from both mitochondrial respiration and glycolysis seems to be the high energy intermediate which is utilized during the cytotoxic activity of macrophages leading to target cell lysis. The process did not depend on concomitant DNA transcription, translation, or protein synthesis.

Full Text

The Full Text of this article is available as a PDF (615.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COHN Z. A., BENSON B. THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. MORPHOLOGY, CYTOCHEMISTRY, AND BIOCHEMISTRY. J Exp Med. 1965 Jan 1;121:153–170. doi: 10.1084/jem.121.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohn Z. A. The regulation of pinocytosis in mouse macrophages. I. Metabolic requirements as defined by the use of inhibitors. J Exp Med. 1966 Oct 1;124(4):557–571. doi: 10.1084/jem.124.4.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Goldstein I., Hoffstein S., Gallin J., Weissmann G. Mechanisms of lysosomal enzyme release from human leukocytes: microtubule assembly and membrane fusion induced by a component of complement. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2916–2920. doi: 10.1073/pnas.70.10.2916. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Melsom H., Seljelid R. The cytotoxic effect of mouse macrophages on syngeneic and allogeneic erythrocytes. J Exp Med. 1973 Mar 1;137(3):807–820. doi: 10.1084/jem.137.3.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Onaya T., Solomon D. H. Effects of chlorpromazine and propranolol on in vitro thyroid activation by thyrotropin, long-acting thyroid stimulator and dibutyryl cyclic-AMP. Endocrinology. 1969 Dec;85(6):1010–1017. doi: 10.1210/endo-85-6-1010. [DOI] [PubMed] [Google Scholar]
  6. Persellin R. H., Ziff M. The effect of gold salt on lysosomal enzymes of the peritoneal macrophage. Arthritis Rheum. 1966 Feb;9(1):57–65. doi: 10.1002/art.1780090107. [DOI] [PubMed] [Google Scholar]
  7. Poste G., Reeve P. Inhibition of cell fusion by local anaesthetics and tranquillizers. Exp Cell Res. 1972 Jun;72(2):556–560. doi: 10.1016/0014-4827(72)90029-8. [DOI] [PubMed] [Google Scholar]
  8. SBARRA A. J., KARNOVSKY M. L. The biochemical basis of phagocytosis. I. Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes. J Biol Chem. 1959 Jun;234(6):1355–1362. [PubMed] [Google Scholar]
  9. SLATER E. C. The components of the dihydrocozymase oxidase system. Biochem J. 1950 Apr;46(4):484–499. doi: 10.1042/bj0460484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Seeman P. M. Membrane stabilization by drugs: tranquilizers, steroids, and anesthetics. Int Rev Neurobiol. 1966;9:145–221. doi: 10.1016/s0074-7742(08)60138-5. [DOI] [PubMed] [Google Scholar]
  11. Seljelid R., Melsom H., Munthekaas A. Lytic effect of mouse macrophages on red cells. Exp Cell Res. 1972 Dec;75(2):534–536. doi: 10.1016/0014-4827(72)90466-1. [DOI] [PubMed] [Google Scholar]
  12. Temple A., Loewi G., Davies P., Howard A. Cytotoxicity of immune guinea-pig cells. II. The mechanism of macrophage cytotoxicity. Immunology. 1973 Apr;24(4):655–669. [PMC free article] [PubMed] [Google Scholar]
  13. Whistler R. L., Lake W. C. Inhibition of cellular transport processes by 5-thio-D-glucopyranose. Biochem J. 1972 Dec;130(4):919–925. doi: 10.1042/bj1300919. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES