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. 1974 Jun 1;139(6):1488–1498. doi: 10.1084/jem.139.6.1488

SYNERGY AMONG RESPONDING LYMPHOID CELLS IN THE ONE-WAY MIXED LYMPHOCYTE REACTION

INTERACTION BETWEEN TWO TYPES OF THYMUS-DEPENDENT CELLS

Wolfgang Tittor 1, Maria Gerbase-Delima 1, Roy L Walford 1
PMCID: PMC2139676  PMID: 4151454

Abstract

The present studies have shown that two subpopulations of thymus-dependent lymphocytes may act synergistically in the mixed lymphocyte reaction (MLR) in the mouse. One subpopulation was well represented in the young adult thymus and the other in lymph nodes. For optimum synergy, both populations must be allogeneic to the stimulator cells. Pretreatment of either population with mitomycin-C abolished synergy. Anti-θ serum abolished both MLR responding and synergizing activities of lymphoid cells. The two thymus-dependent subpopulations were both present in the spleen, and displayed different migratory patterns when injected into irradiated mice: one population went to spleens of the irradiated mice, the other to lymph nodes. The effects of anti-thymocyte serum on the MLR and upon synergy were assessed. While minor differences exist and are herein described, our overall results strongly suggest that in our experiments with synergy in MLR, we may be dealing with the same T1- and T2-cell subpopulations described by Cantor and Asofsky and coworkers (1, 2, 4, 5, 14) as displaying synergy in the graft-vs.-host reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cantor H., Asofsky R. Synergy among lymphoid cells mediating the graft-versus-host response. 3. Evidence for interaction between two types of thymus-derived cells. J Exp Med. 1972 Apr 1;135(4):764–779. doi: 10.1084/jem.135.4.764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cantor H., Asofsky R. Synergy among lymphoid cells mediating the graft-versus-host response. II. Synergy in graft-versus-host reactions produced by Balb-c lymphoid cells of differing anatomic origin. J Exp Med. 1970 Feb;131(2):235–246. doi: 10.1084/jem.131.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cantor H., Asofsky R., Talal N. Synergy among lymphoid cells mediating the graft-versus-host response. I. Synergy in graft-versus-host reactions produced by cells from NZB-Bl mice. J Exp Med. 1970 Feb;131(2):223–234. doi: 10.1084/jem.131.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cantor H., Mosier D. E. Maturation of reactivity to histocompatibility antigens. Transplant Proc. 1972 Jun;4(2):159–162. [PubMed] [Google Scholar]
  5. Cantor H. The effects of anti-theta antiserum upon graft-versus-host activity of spleen and lymph node cells. Cell Immunol. 1972 Mar;3(3):461–469. doi: 10.1016/0008-8749(72)90251-1. [DOI] [PubMed] [Google Scholar]
  6. Cohen L., Howe M. L. Synergism between subpopulations of thymus-derived cells mediating the proliferative and effector phases of the mixed lymphocyte reaction. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2707–2710. doi: 10.1073/pnas.70.9.2707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Elkins W. L. Cellular immunology and the pathogenesis of graft versus host reactions. Prog Allergy. 1971;15:78–187. [PubMed] [Google Scholar]
  8. Lance E. M. The selective action of antilymphocyte serum on recirculating lymphocytes: a review of the evidence and alternatives. Clin Exp Immunol. 1970 Jun;6(6):789–802. [PMC free article] [PubMed] [Google Scholar]
  9. Levey R. H., Medawar P. B. Nature and mode of action of antilymphocytic antiserum. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1130–1137. doi: 10.1073/pnas.56.4.1130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Raff M. C. Surface antigenic markers for distinguishing T and B lymphocytes in mice. Transplant Rev. 1971;6:52–80. doi: 10.1111/j.1600-065x.1971.tb00459.x. [DOI] [PubMed] [Google Scholar]
  11. Stutman O. Lymphocyte subpopulations in NZB mice: deficit of thymus-dependent lymphocytes. J Immunol. 1972 Sep;109(3):602–611. [PubMed] [Google Scholar]
  12. Taub R. N., Lance E. M. Effects of lymphoid depletion on the distribution of 51Cr-labeled lymph node cells in mice. Transplantation. 1971 Jun;11(6):536–542. [PubMed] [Google Scholar]
  13. Tigelaar R. E., Asofsky R. Synergy among lymphoid cells mediating the graft-versus-host response. V. Derivation by migration in lethally irradiated recipients of two interacting subpopulations of thymus-derived cells from normal spleen. J Exp Med. 1973 Feb 1;137(2):239–253. doi: 10.1084/jem.137.2.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tittor W., Walford R. L. Synergistic response between thymus and lymph node cells in the mixed lymphocyte culture. Nature. 1974 Feb 8;247(5440):371–373. doi: 10.1038/247371a0. [DOI] [PubMed] [Google Scholar]
  15. Wagner H. Synergy during in vitro cytotoxic allograft responses. I. Evidence for cell interaction between thymocytes and peripheral T cells. J Exp Med. 1973 Dec 1;138(6):1379–1397. doi: 10.1084/jem.138.6.1379. [DOI] [PMC free article] [PubMed] [Google Scholar]

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