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. 1974 Jul 1;140(1):172–184. doi: 10.1084/jem.140.1.172

GENETIC CONTROL OF IMMUNE RESPONSES IN VITRO

III. TOLEROGENIC PROPERTIES OF THE TERPOLYMERL-GLUTAMIC ACID60-L-ALANINE30-L-TYROSINE10 (GAT) FOR SPLEEN CELLS FROM NONRESPONDER (H-2qANDH-2s) MICE

Judith A Kapp 1, Carl W Pierce 1, Baruj Benacerraf 1
PMCID: PMC2139710  PMID: 4857865

Abstract

Although nonresponder, H-2s and H-2q, mice fail to develop GAT-specific PFC responses to GAT, they do develop GAT-specific PFC responses when stimulated by GAT complexed to an immunogenic carrier such as methylated bovine serum albumin. The studies described in this paper show that injection of nonresponder mice with GAT specifically decreases their ability to develop anti-GAT PFC responses to a subsequent challenge with GAT-MBSA. Addition of GAT to cultures of spleen cells from nonresponder mice also prevents development of the GAT-specific PFC responses stimulated by GAT-MBSA. Thus, interaction of nonresponder spleen cells with GAT leads to the induction of unresponsiveness in vivo and in vitro. Various parameters of the tolerance induction have been investigated and described. A comparison of the effects of GAT on B cells indicates that nonresponder B cells are more readily rendered unresponsive by soluble GAT than are responder B cells. The significance of these data for our understanding of Ir gene regulation of the immune response is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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