Abstract
These studies demonstrate that mice tolerant to human gamma globulin (HGG) regain their ability to make antibody to HGG after parabiosis to normal mice. This can be demonstrated by enumeration of PFC in the spleens of both the normal and tolerant partners. Hemagglutinin titers of normal-tolerant parabionts, however, are exceptionally low; serum antibody appears to be neutralized by circulating HGG present originally in the serum of the tolerant partner. These data support the hypothesis that tolerance to HGG in mice is a "defective" state due to the absence of cells capable of responding to this antigen.
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Selected References
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- Avrameas S., Taudou B., Chuilon S. Glutaraldehyde, cyanuric chloride and tetrazotized O-dianisidine as coupling reagents in the passive hemagglutination test. Immunochemistry. 1969 Jan;6(1):67–76. doi: 10.1016/0019-2791(69)90179-7. [DOI] [PubMed] [Google Scholar]
- Brent L., Brooks C., Lubling N., Thomas A. V. Attempts to demonstrate an in vivo role for serum blocking factors in tolerant mice. Transplantation. 1972 Sep;14(3):382–387. doi: 10.1097/00007890-197209000-00016. [DOI] [PubMed] [Google Scholar]
- Chiller J. M., Weigle W. O. Termination of tolerance to human gamma globulin in mice by antigen and bacterial lipopolysaccharide (endotoxin). J Exp Med. 1973 Mar 1;137(3):740–750. doi: 10.1084/jem.137.3.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EICHWALD E. J., LUSTGRAAF E. C., STRAINER M. Genetic factors in parabiosis. J Natl Cancer Inst. 1959 Dec;23:1193–1213. [PubMed] [Google Scholar]
- Golub E. S., Mishell R. I., Weigle W. O., Dutton R. W. A modification of the hemolytic plaque assay for use with protein antigens. J Immunol. 1968 Jan;100(1):133–137. [PubMed] [Google Scholar]
- Golub E. S., Weigle W. O. Studies on the induction of immunologic unresponsiveness. 3. Antigen form and mouse strain variation. J Immunol. 1969 Feb;102(2):389–396. [PubMed] [Google Scholar]
- MARTINEZ C., SHAPIRO F., KELMAN H., ONSTAD T., GOOD R. A. Tolerance of F1 hybrid skin homografts in the parent strain induced by parabiosis. Proc Soc Exp Biol Med. 1960 Feb;103:266–269. doi: 10.3181/00379727-103-25483. [DOI] [PubMed] [Google Scholar]
- McCullagh P. J. The abrogation of sheep erythrocyte tolerance in rats by means of the transfer of allogeneic lymphocytes. J Exp Med. 1970 Nov;132(5):916–925. doi: 10.1084/jem.132.5.916. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Naor D., Sulitzeanu D. Binding of 125I-BSA to lymphoid cells of tolerant mice. Int Arch Allergy Appl Immunol. 1969;36(1):112–113. doi: 10.1159/000230728. [DOI] [PubMed] [Google Scholar]
- Tong J. L., Boose D. Immunosuppressive effect of serum from CBA mice made tolerant by the subernatant from ultracentrifuged bovine gamma globulin. J Immunol. 1970 Aug;105(2):426–430. [PubMed] [Google Scholar]