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. 1987 Dec;169(12):5715–5720. doi: 10.1128/jb.169.12.5715-5720.1987

Cloning of whiG, a gene critical for sporulation of Streptomyces coelicolor A3(2).

C Mendez 1, K F Chater 1
PMCID: PMC214075  PMID: 3680175

Abstract

In whiG mutants of Streptomyces coelicolor A3(2), aerial hyphae do not show any sign of sporulation. A library of S. coelicolor DNA was prepared in a phi C31 temperate phage vector (KC516), and one recombinant phage (KC750) that could restore the wild-type phenotype to a collection of whiG mutants when integrated into their genomes was found. Subcloning experiments with low- and high-copy-number Streptomyces plasmid vectors allowed partial localization of whiG in the cloned DNA and revealed that hypersporulation was associated with the presence of extra copies of whiG.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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