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. 1935 Mar 20;18(4):485–490. doi: 10.1085/jgp.18.4.485

FREEZING POINTS OF ANTI-COAGULANT SALT SOLUTIONS

David I Hitchcock 1, Ruth B Dougan 1
PMCID: PMC2141367  PMID: 19872860

Abstract

By a method involving equilibration of ice and solution, and analysis of the solution, freezing point depressions of solutions of sodium citrate, oxalate, and fluoride have been determined over the range Δ = 0.45 to 0.65°C. Determinations with sodium chloride solutions have confirmed the accuracy of the method. In each case the freezing point depression is given, within 0.002°C., as a linear function of the concentration. By the use of these linear equations it is possible to prepare a solution of any of these four salts isotonic with a given biological fluid of known freezing point, provided the latter falls within the range studied.

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  1. Rous P., Turner J. R. THE PRESERVATION OF LIVING RED BLOOD CELLS IN VITRO : I. METHODS OF PRESERVATION. J Exp Med. 1916 Feb 1;23(2):219–237. doi: 10.1084/jem.23.2.219. [DOI] [PMC free article] [PubMed] [Google Scholar]

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