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. 1936 May 20;19(5):739–752. doi: 10.1085/jgp.19.5.739

THE STRUCTURE OF THE ULTRAVIOLET ABSORPTION SPECTRA OF CERTAIN PROTEINS AND AMINO ACIDS

Calvin B Coulter 1, Florence M Stone 1, Elvin A Kabat 1
PMCID: PMC2141464  PMID: 19872958

Abstract

1. The absorption spectra of a number of proteins in the region 2500 to 3000 A. have been found to comprise from six to nine narrow bands. In consequence of variation in the relative intensity of these bands from protein to protein, the absorption curve has a characteristic configuration for each protein. 2. These bands correspond closely in position with the narrow bands which appear in the absorption spectra of tryptophan, tyrosin, and phenylalanine. Tryptophan and tyrosin each present three bands, phenylalanine shows nine. 3. The bands in the proteins are accordingly attributed to these amino acids. In the proteins the bands are displaced from the positions which they occupy in the uncombined amino acids, in most instances, by 10 to 35 A. toward longer wavelengths. 4. The absorption spectrum of Pneumococcus Type I antibody resembles that of normal pseudoglobulin but shows characteristic differences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chow B. F., Goebel W. F. THE PURIFICATION OF THE ANTIBODIES IN TYPE I ANTI-PNEUMOCOCCUS SERUM, AND THE CHEMICAL NATURE OF THE TYPE-SPECIFIC PRECIPITIN REACTION. J Exp Med. 1935 Jul 31;62(2):179–202. doi: 10.1084/jem.62.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ward F. W. The Absorption Spectra of some Amino Acids: The Possible Ring Structure of Cystine. Biochem J. 1923;17(6):898–902. doi: 10.1042/bj0170898. [DOI] [PMC free article] [PubMed] [Google Scholar]

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