Abstract
The nucleotide sequences of the genes encoding the subunits of Klebsiella pneumoniae and Salmonella typhimurium type 1 fimbriae were determined. Comparison of the predicted amino acid sequences of the two subunits revealed domains in which the sequences were highly conserved. Both gene products possessed signal peptides, a fact consistent with the transport of the fimbrial subunit across the membrane, but these regions showed no amino acid homology between the two proteins. The predicted N-terminal amino acid sequences of the processed fimbrial subunits were in good agreement with those obtained by purification of the fimbrial subunits.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abraham J. M., Freitag C. S., Clements J. R., Eisenstein B. I. An invertible element of DNA controls phase variation of type 1 fimbriae of Escherichia coli. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5724–5727. doi: 10.1073/pnas.82.17.5724. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brenner D. J. Characterization and clinical identification of Enterobacteriaceae by DNA hybridization. Prog Clin Pathol. 1978;7:71–117. [PubMed] [Google Scholar]
- Chou P. Y., Fasman G. D. Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978;47:45–148. doi: 10.1002/9780470122921.ch2. [DOI] [PubMed] [Google Scholar]
- Clegg S., Hull S., Hull R., Pruckler J. Construction and comparison of recombinant plasmids encoding type 1 fimbriae of members of the family Enterobacteriaceae. Infect Immun. 1985 May;48(2):275–279. doi: 10.1128/iai.48.2.275-279.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duguid J. P., Campbell I. Antigens of the type-1 fimbriae of salmonellae and other enterobacteria. J Med Microbiol. 1969 Nov 4;2(4):535–553. doi: 10.1099/00222615-2-4-535. [DOI] [PubMed] [Google Scholar]
- Fader R. C., Duffy L. K., Davis C. P., Kurosky A. Purification and chemical characterization of type 1 pili isolated from Klebsiella pneumoniae. J Biol Chem. 1982 Mar 25;257(6):3301–3305. [PubMed] [Google Scholar]
- GILLIES R. R., DUGUID J. P. The fimbrial antigens of Shigella flexneri. J Hyg (Lond) 1958 Sep;56(3):303–318. doi: 10.1017/s0022172400037803. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klemm P. The fimA gene encoding the type-1 fimbrial subunit of Escherichia coli. Nucleotide sequence and primary structure of the protein. Eur J Biochem. 1984 Sep 3;143(2):395–399. doi: 10.1111/j.1432-1033.1984.tb08386.x. [DOI] [PubMed] [Google Scholar]
- Maurer L., Orndorff P. E. Identification and characterization of genes determining receptor binding and pilus length of Escherichia coli type 1 pili. J Bacteriol. 1987 Feb;169(2):640–645. doi: 10.1128/jb.169.2.640-645.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Michaelis S., Beckwith J. Mechanism of incorporation of cell envelope proteins in Escherichia coli. Annu Rev Microbiol. 1982;36:435–465. doi: 10.1146/annurev.mi.36.100182.002251. [DOI] [PubMed] [Google Scholar]
- Minion F. C., Abraham S. N., Beachey E. H., Goguen J. D. The genetic determinant of adhesive function in type 1 fimbriae of Escherichia coli is distinct from the gene encoding the fimbrial subunit. J Bacteriol. 1986 Mar;165(3):1033–1036. doi: 10.1128/jb.165.3.1033-1036.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nowotarska M., Mulczyk M. Serologic relationship of fimbriae among Enterobacteriaceae. Arch Immunol Ther Exp (Warsz) 1977;25(1):7–16. [PubMed] [Google Scholar]
- Orndorff P. E., Falkow S. Nucleotide sequence of pilA, the gene encoding the structural component of type 1 pili in Escherichia coli. J Bacteriol. 1985 Apr;162(1):454–457. doi: 10.1128/jb.162.1.454-457.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Purcell B. K., Clegg S. Construction and expression of recombinant plasmids encoding type 1 fimbriae of a urinary Klebsiella pneumoniae isolate. Infect Immun. 1983 Mar;39(3):1122–1127. doi: 10.1128/iai.39.3.1122-1127.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spears P. A., Schauer D., Orndorff P. E. Metastable regulation of type 1 piliation in Escherichia coli and isolation and characterization of a phenotypically stable mutant. J Bacteriol. 1986 Oct;168(1):179–185. doi: 10.1128/jb.168.1.179-185.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]