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. 1985 Oct;164(1):359–366. doi: 10.1128/jb.164.1.359-366.1985

Cloning and expression of bacterial ice nucleation genes in Escherichia coli.

C Orser, B J Staskawicz, N J Panopoulos, D Dahlbeck, S E Lindow
PMCID: PMC214252  PMID: 3900043

Abstract

Epiphytic populations of Pseudomonas syringae and Erwinia herbicola are important sources of ice nuclei that incite frost damage in agricultural crop plants. We have cloned and characterized DNA segments carrying the genes (ice) responsible for the ice-nucleating ability of these bacteria. The ice region spanned 3.5 to 4.0 kilobases and was continuous over this region in P. syringae Cit7R1. The cloned fragments imparted ice-nucleating activity in Escherichia coli. Substantial increases in the nucleating activity of both E. coli and P. syringae were obtained by subcloning the DNA fragments on multicopy plasmid vectors. Southern blot analysis showed substantial homology between the ice regions of P. syringae and E. herbicola, although individual restriction sites within the ice regions differed between the two species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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