Abstract
The structure and function of the periplasmic heme-binding protein HbpA of Haemophilus influenzae were investigated. This protein is involved in the import of heme into the bacteria through the inner membrane, and thus is a key element of the organism's ability to survive in blood. A high degree of sequence similarity between HbpA and the dipeptide-binding protein of Escherichia coli is suggested to be the result of a functional relationship. An HbpA model built using the dipeptide-binding protein suggests a mode of heme binding that is distinct from those known in proteins of the human host. These results provide a starting point for rational drug design.
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- Abouhamad W. N., Manson M. D. The dipeptide permease of Escherichia coli closely resembles other bacterial transport systems and shows growth-phase-dependent expression. Mol Microbiol. 1994 Dec;14(5):1077–1092. doi: 10.1111/j.1365-2958.1994.tb01340.x. [DOI] [PubMed] [Google Scholar]
- Abouhamad W. N., Manson M., Gibson M. M., Higgins C. F. Peptide transport and chemotaxis in Escherichia coli and Salmonella typhimurium: characterization of the dipeptide permease (Dpp) and the dipeptide-binding protein. Mol Microbiol. 1991 May;5(5):1035–1047. doi: 10.1111/j.1365-2958.1991.tb01876.x. [DOI] [PubMed] [Google Scholar]
- Bernstein F. C., Koetzle T. F., Williams G. J., Meyer E. F., Jr, Brice M. D., Rodgers J. R., Kennard O., Shimanouchi T., Tasumi M. The Protein Data Bank: a computer-based archival file for macromolecular structures. J Mol Biol. 1977 May 25;112(3):535–542. doi: 10.1016/s0022-2836(77)80200-3. [DOI] [PubMed] [Google Scholar]
- Björkman A. J., Binnie R. A., Cole L. B., Zhang H., Hermodson M. A., Mowbray S. L. Identical mutations at corresponding positions in two homologous proteins with nonidentical effects. J Biol Chem. 1994 Apr 15;269(15):11196–11200. [PubMed] [Google Scholar]
- Chothia C., Lesk A. M. The relation between the divergence of sequence and structure in proteins. EMBO J. 1986 Apr;5(4):823–826. doi: 10.1002/j.1460-2075.1986.tb04288.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cope L. D., Thomas S. E., Latimer J. L., Slaughter C. A., Müller-Eberhard U., Hansen E. J. The 100 kDa haem:haemopexin-binding protein of Haemophilus influenzae: structure and localization. Mol Microbiol. 1994 Sep;13(5):863–873. doi: 10.1111/j.1365-2958.1994.tb00478.x. [DOI] [PubMed] [Google Scholar]
- Dunten P., Mowbray S. L. Crystal structure of the dipeptide binding protein from Escherichia coli involved in active transport and chemotaxis. Protein Sci. 1995 Nov;4(11):2327–2334. doi: 10.1002/pro.5560041110. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guyer C. A., Morgan D. G., Osheroff N., Staros J. V. Purification and characterization of a periplasmic oligopeptide binding protein from Escherichia coli. J Biol Chem. 1985 Sep 5;260(19):10812–10818. [PubMed] [Google Scholar]
- Hanson M. S., Hansen E. J. Molecular cloning, partial purification, and characterization of a haemin-binding lipoprotein from Haemophilus influenzae type b. Mol Microbiol. 1991 Feb;5(2):267–278. doi: 10.1111/j.1365-2958.1991.tb02107.x. [DOI] [PubMed] [Google Scholar]
- Hanson M. S., Slaughter C., Hansen E. J. The hbpA gene of Haemophilus influenzae type b encodes a heme-binding lipoprotein conserved among heme-dependent Haemophilus species. Infect Immun. 1992 Jun;60(6):2257–2266. doi: 10.1128/iai.60.6.2257-2266.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Manson M. D., Blank V., Brade G., Higgins C. F. Peptide chemotaxis in E. coli involves the Tap signal transducer and the dipeptide permease. Nature. 1986 May 15;321(6067):253–256. doi: 10.1038/321253a0. [DOI] [PubMed] [Google Scholar]
- Mowbray S. L. Ribose and glucose-galactose receptors. Competitors in bacterial chemotaxis. J Mol Biol. 1992 Sep 20;227(2):418–440. doi: 10.1016/0022-2836(92)90898-t. [DOI] [PubMed] [Google Scholar]
- Olson E. R., Dunyak D. S., Jurss L. M., Poorman R. A. Identification and characterization of dppA, an Escherichia coli gene encoding a periplasmic dipeptide transport protein. J Bacteriol. 1991 Jan;173(1):234–244. doi: 10.1128/jb.173.1.234-244.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ponder J. W., Richards F. M. Tertiary templates for proteins. Use of packing criteria in the enumeration of allowed sequences for different structural classes. J Mol Biol. 1987 Feb 20;193(4):775–791. doi: 10.1016/0022-2836(87)90358-5. [DOI] [PubMed] [Google Scholar]
- Tame J. R., Murshudov G. N., Dodson E. J., Neil T. K., Dodson G. G., Higgins C. F., Wilkinson A. J. The structural basis of sequence-independent peptide binding by OppA protein. Science. 1994 Jun 10;264(5165):1578–1581. doi: 10.1126/science.8202710. [DOI] [PubMed] [Google Scholar]
- de Pina K., Navarro C., McWalter L., Boxer D. H., Price N. C., Kelly S. M., Mandrand-Berthelot M. A., Wu L. F. Purification and characterization of the periplasmic nickel-binding protein NikA of Escherichia coli K12. Eur J Biochem. 1995 Feb 1;227(3):857–865. doi: 10.1111/j.1432-1033.1995.tb20211.x. [DOI] [PubMed] [Google Scholar]