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. 1995 Nov;4(11):2289–2299. doi: 10.1002/pro.5560041106

Comparison of the accuracy of protein solution structures derived from conventional and network-edited NOESY data.

C G Hoogstraten 1, S Choe 1, W M Westler 1, J L Markley 1
PMCID: PMC2143029  PMID: 8563625

Abstract

Network-editing experiments are variants of the basic NOESY experiment that allow more accurate direct measurement of interproton distances in macromolecules by defeating specific spin-diffusion pathways. Two network-editing approaches, block-decoupled NOESY and complementary-block-decoupled-NOESY, were applied as three-dimensional, heteronuclear-edited experiments to distance measurement in a small protein, turkey ovomucoid third domain (OMTKY3). Two-hundred and twelve of the original 655 distance constraints observed in this molecule (Krezel AM et al., 1994, J Mol Biol 242:203-214) were improved by their replacement by distances derived from network-edited spectra, and distance geometry/simulated annealing solution structure calculations were performed from both the unimproved and improved distance sets. The resulting two families of structures were found to differ significantly, the most important differences being the hinge angle of a beta-turn and an expansion of the sampled conformation space in the region of the reactive-site loop. The structures calculated from network-editing data are interpreted as a more accurate model of the solution conformation of OMTKY3.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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