Abstract
We report the presence of a single L-asparagine aminohydrolase activity (EC 3.5.1.1) in extracts of Bacillus licheniformis A5. The synthesis of the enzyme was apparently under nitrogen catabolite repression control.
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Selected References
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- Campbell H. A., Mashburn L. T., Boyse E. A., Old L. J. Two L-asparaginases from Escherichia coli B. Their separation, purification, and antitumor activity. Biochemistry. 1967 Mar;6(3):721–730. doi: 10.1021/bi00855a011. [DOI] [PubMed] [Google Scholar]
- Cedar H., Schwartz J. H. Localization of the two-L-asparaginases in anaerobically grown Escherichia coli. J Biol Chem. 1967 Aug 25;242(16):3753–3755. [PubMed] [Google Scholar]
- Cedar H., Schwartz J. H. Production of L-asparaginase II by Escherichia coli. J Bacteriol. 1968 Dec;96(6):2043–2048. doi: 10.1128/jb.96.6.2043-2048.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cook W. R., Hoffman J. H., Bernlohr R. W. Occurrence of an inducible glutaminase in Bacillus licheniformis. J Bacteriol. 1981 Oct;148(1):365–367. doi: 10.1128/jb.148.1.365-367.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Del Casale T., Sollitti P., Chesney R. H. Cytoplasmic L-asparaginase: isolation of a defective strain and mapping of ansA. J Bacteriol. 1983 Apr;154(1):513–515. doi: 10.1128/jb.154.1.513-515.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donohue T. J., Bernlohr R. W. Effect of cultural conditions on the concentrations of metabolic intermediates during growth and sporulation of Bacillus licheniformis. J Bacteriol. 1978 Aug;135(2):363–372. doi: 10.1128/jb.135.2.363-372.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dunlop P. C., Meyer G. M., Roon R. J. Nitrogen catabolite repression of asparaginase II in Saccharomyces cerevisiae. J Bacteriol. 1980 Jul;143(1):422–426. doi: 10.1128/jb.143.1.422-426.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fields P. I., Schreier H. J., Saha A. L., Bernlohr R. W. Use of the beta-lactamase inhibitor clavulanic acid in the isolation of auxotrophic mutants of Bacillus licheniformis. J Bacteriol. 1984 Aug;159(2):803–804. doi: 10.1128/jb.159.2.803-804.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kalb V. F., Jr, Bernlohr R. W. A new spectrophotometric assay for protein in cell extracts. Anal Biochem. 1977 Oct;82(2):362–371. doi: 10.1016/0003-2697(77)90173-7. [DOI] [PubMed] [Google Scholar]
- Marzluf G. A. Regulation of nitrogen metabolism and gene expression in fungi. Microbiol Rev. 1981 Sep;45(3):437–461. doi: 10.1128/mr.45.3.437-461.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meile L., Soldati L., Leisinger T. Regulation of proline catabolism in Pseudomonas aeruginosa PAO. Arch Microbiol. 1982 Aug;132(2):189–193. doi: 10.1007/BF00508729. [DOI] [PubMed] [Google Scholar]
- O'Reilly T., Day D. F. Effects of cultural conditions on protease production by Aeromonas hydrophila. Appl Environ Microbiol. 1983 Mar;45(3):1132–1135. doi: 10.1128/aem.45.3.1132-1135.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Prusiner S., Milner L. A rapid radioactive assay for glutamine synthetase, glutaminase, asparagine synthetase, and asparaginase. Anal Biochem. 1970 Oct;37(2):429–438. doi: 10.1016/0003-2697(70)90069-2. [DOI] [PubMed] [Google Scholar]
- Schreier H. J., Smith T. M., Bernlohr R. W. Regulation of nitrogen catabolic enzymes in Bacillus spp. J Bacteriol. 1982 Aug;151(2):971–975. doi: 10.1128/jb.151.2.971-975.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tyler B. Regulation of the assimilation of nitrogen compounds. Annu Rev Biochem. 1978;47:1127–1162. doi: 10.1146/annurev.bi.47.070178.005403. [DOI] [PubMed] [Google Scholar]