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. 1985 Nov;164(2):947–949. doi: 10.1128/jb.164.2.947-949.1985

DNA supercoiling and suppression of the leu-500 promoter mutation.

G J Pruss, K Drlica
PMCID: PMC214348  PMID: 2997140

Abstract

top mutations (formerly supX) eliminate DNA topoisomerase I activity and suppress the leu-500 promoter mutation in Salmonella typhimurium (K. M. Overbye, S. K. Basu, and P. Margolin, Cold Spring Harbor Symp. Quant. Biol. 47:785-791, 1983). Sublethal doses of coumermycin which reduce intracellular levels of supercoiling activity in a top mutant eliminated suppression of the leu-500 mutation. This result provides evidence that increased DNA supercoiling suppresses the leu-500 promoter mutation in top mutants.

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Selected References

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