Abstract
Genes coding for phosphoribulokinase (PRK), a key enzyme of the Calvin cycle, were localized in the genome of the chemoautotroph Alcaligenes eutrophus. The NH2-terminal sequence of the PRK subunit was determined. With a synthetic oligodeoxynucleotide probe complementary to a portion of this sequence, hybridization analysis revealed PRK genes to be located on both the chromosome and the megaplasmid pHG1 of A. eutrophus H16.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andersen K., Wilke-Douglas M. Construction and use of a gene bank of Alcaligenes eutrophus in the analysis of ribulose bisphosphate carboxylase genes. J Bacteriol. 1984 Sep;159(3):973–978. doi: 10.1128/jb.159.3.973-978.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chang J. Y., Lehmann A., Wittmann-Liebold B. Analysis of dimethylaminoazobenzene-thiohydantoins of amino acid by high-pressure liquid chromatography. Anal Biochem. 1980 Mar 1;102(2):380–383. doi: 10.1016/0003-2697(80)90171-2. [DOI] [PubMed] [Google Scholar]
- Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
- Friedrich B., Hogrefe C., Schlegel H. G. Naturally occurring genetic transfer of hydrogen-oxidizing ability between strains of Alcaligenes eutrophus. J Bacteriol. 1981 Jul;147(1):198–205. doi: 10.1128/jb.147.1.198-205.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hogrefe C., Römermann D., Friedrich B. Alcaligenes eutrophus hydrogenase genes (Hox). J Bacteriol. 1984 Apr;158(1):43–48. doi: 10.1128/jb.158.1.43-48.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
- Salnikow J., Lehmann A., Wittmann-Liebold B. Improved automated solid-phase microsequencing of peptides using DABITC. Anal Biochem. 1981 Nov 1;117(2):433–442. doi: 10.1016/0003-2697(81)90803-4. [DOI] [PubMed] [Google Scholar]
- Siebert K., Schobert P., Bowien B. Purification, some catalytic and molecular properties of phosphoribulokinase from Alcaligenes eutrophus. Biochim Biophys Acta. 1981 Mar 13;658(1):35–44. doi: 10.1016/0005-2744(81)90247-3. [DOI] [PubMed] [Google Scholar]
- Wachter E., Machleidt W., Hofner H., Otto J. Aminopropyl glass and its p-phenylene diisothiocyanate derivative, a new support in solid-phase Edman degradation of peptides and proteins. FEBS Lett. 1973 Sep 1;35(1):97–102. doi: 10.1016/0014-5793(73)80585-x. [DOI] [PubMed] [Google Scholar]
- Wallace R. B., Johnson M. J., Hirose T., Miyake T., Kawashima E. H., Itakura K. The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA. Nucleic Acids Res. 1981 Feb 25;9(4):879–894. doi: 10.1093/nar/9.4.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wallace R. B., Shaffer J., Murphy R. F., Bonner J., Hirose T., Itakura K. Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch. Nucleic Acids Res. 1979 Aug 10;6(11):3543–3557. doi: 10.1093/nar/6.11.3543. [DOI] [PMC free article] [PubMed] [Google Scholar]