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. 1985 Nov;164(2):964–968. doi: 10.1128/jb.164.2.964-968.1985

Regulation of inorganic phosphate transport systems in Saccharomyces cerevisiae.

Y Tamai, A Toh-e, Y Oshima
PMCID: PMC214353  PMID: 3902805

Abstract

A kinetic study of Pi transport with 32Pi revealed that Saccharomyces cerevisiae has two systems of Pi transport, one with a low Km value (8.2 microM) for external Pi and the other with a high Km value (770 microM). The low-Km system was derepressed by Pi starvation, and the activity was expressed under the control of a genetic system which regulates the repressible acid and alkaline phosphatases. The function of the PHO2 gene, which is essential for the derepression of repressible acid phosphatase but not for the derepression of repressible alkaline phosphatase, was also indispensable for the derepression of the low-Km system.

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Selected References

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  1. Adams B. G. Method for decryptification of -glucosidase in yeast with dimethyl sulfoxide. Anal Biochem. 1972 Jan;45(1):137–146. doi: 10.1016/0003-2697(72)90014-0. [DOI] [PubMed] [Google Scholar]
  2. Argast M., Boos W. Co-regulation in Escherichia coli of a novel transport system for sn-glycerol-3-phosphate and outer membrane protein Ic (e, E) with alkaline phosphatase and phosphate-binding protein. J Bacteriol. 1980 Jul;143(1):142–150. doi: 10.1128/jb.143.1.142-150.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bostian K. A., Lemire J. M., Halvorson H. O. Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae. Mol Cell Biol. 1983 May;3(5):839–853. doi: 10.1128/mcb.3.5.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KATCHMAN B. J., FETTY W. O. Phosphorus metabolism in growing cultures of Saccharomyces cerevisiae. J Bacteriol. 1955 Jun;69(6):607–615. doi: 10.1128/jb.69.6.607-615.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kaneko Y., Tamai Y., Toh-e A., Oshima Y. Transcriptional and post-transcriptional control of PHO8 expression by PHO regulatory genes in Saccharomyces cerevisiae. Mol Cell Biol. 1985 Jan;5(1):248–252. doi: 10.1128/mcb.5.1.248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kaneko Y., Toh-e A., Oshima Y. Identification of the genetic locus for the structural gene and a new regulatory gene for the synthesis of repressible alkaline phosphatase in Saccharomyces cerevisiae. Mol Cell Biol. 1982 Feb;2(2):127–137. doi: 10.1128/mcb.2.2.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kramer R. A., Andersen N. Isolation of yeast genes with mRNA levels controlled by phosphate concentration. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6541–6545. doi: 10.1073/pnas.77.11.6541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lindegren G., Hwang Y. L., Oshima Y., Lindegren C. C. Genetical mutants induced by ethyl methanesulfonate in Saccharomyces. Can J Genet Cytol. 1965 Sep;7(3):491–499. doi: 10.1139/g65-064. [DOI] [PubMed] [Google Scholar]
  9. Lowendorf H. S., Slayman C. W. Genetic regulation of phosphate transport system II in Neurospora. Biochim Biophys Acta. 1975 Nov 17;413(1):95–103. doi: 10.1016/0005-2736(75)90061-9. [DOI] [PubMed] [Google Scholar]
  10. Rogers D. T., Lemire J. M., Bostian K. A. Acid phosphatase polypeptides in Saccharomyces cerevisiae are encoded by a differentially regulated multigene family. Proc Natl Acad Sci U S A. 1982 Apr;79(7):2157–2161. doi: 10.1073/pnas.79.7.2157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roomans G. M., Blasco F., Borst-Pauwels G. W. Cotransport of phosphate and sodium by yeast. Biochim Biophys Acta. 1977 May 16;467(1):65–71. doi: 10.1016/0005-2736(77)90242-5. [DOI] [PubMed] [Google Scholar]
  12. Toh-E A., Nakamura H., Oshima Y. A gene controlling the synthesis of non specific alkaline phosphatase in Saccharomyces cerevisiae. Biochim Biophys Acta. 1976 Mar 25;428(1):182–192. doi: 10.1016/0304-4165(76)90119-7. [DOI] [PubMed] [Google Scholar]
  13. Toh-E A., Oshima Y. Characterization of a dominant, constitutive mutation, PHOO, for the repressible acid phosphatase synthesis in Saccharomyces cerevisiae. J Bacteriol. 1974 Nov;120(2):608–617. doi: 10.1128/jb.120.2.608-617.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Toh-e A., Inouye S., Oshima Y. Structure and function of the PHO82-pho4 locus controlling the synthesis of repressible acid phosphatase of Saccharomyces cerevisiae. J Bacteriol. 1981 Jan;145(1):221–232. doi: 10.1128/jb.145.1.221-232.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tommassen J., Lugtenberg B. Outer membrane protein e of Escherichia coli K-12 is co-regulated with alkaline phosphatase. J Bacteriol. 1980 Jul;143(1):151–157. doi: 10.1128/jb.143.1.151-157.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ueda Y., Oshima Y. A constitutive mutation, phoT, of the repressible acid phosphatase synthesis with inability to transport inorganic phosphate in Saccharomyces cerevisiae. Mol Gen Genet. 1975;136(3):255–259. doi: 10.1007/BF00334020. [DOI] [PubMed] [Google Scholar]
  17. Ueda Y., To-E A., Oshima Y. Isolation and characterization of recessive, constitutive mutations for repressible acid phosphatase synthesis in Saccharomyces cerevisiae. J Bacteriol. 1975 Jun;122(3):911–922. doi: 10.1128/jb.122.3.911-922.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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