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. 1997 Jul;6(7):1511–1515. doi: 10.1002/pro.5560060715

Tetranectin, a trimeric plasminogen-binding C-type lectin.

T L Holtet 1, J H Graversen 1, I Clemmensen 1, H C Thøgersen 1, M Etzerodt 1
PMCID: PMC2143742  PMID: 9232652

Abstract

Tetranectin, a plasminogen-binding protein belonging to the family of C-type lectins, was expressed in E. coli and converted to its native form by in vitro refolding and proteolytic processing. Recombinant tetranectin-as well as natural tetranectin from human plasma-was shown by chemical cross-linking analysis and SDS-PAGE to be a homo-trimer in solution as are other known members of the collectin family of C-type lectins. Biochemical evidence is presented showing that an N-terminal domain encoded within exons 1 and 2 of the tetranectin gene is necessary and sufficient to govern subunit trimerization.

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Selected References

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