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. 1986 Feb;165(2):341–347. doi: 10.1128/jb.165.2.341-347.1986

Site-specific recombinases: changing partners and doing the twist.

P Sadowski
PMCID: PMC214421  PMID: 3003022

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdel-Meguid S. S., Grindley N. D., Templeton N. S., Steitz T. A. Cleavage of the site-specific recombination protein gamma delta resolvase: the smaller of two fragments binds DNA specifically. Proc Natl Acad Sci U S A. 1984 Apr;81(7):2001–2005. doi: 10.1073/pnas.81.7.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Abremski K., Gottesman S. Purification of the bacteriophage lambda xis gene product required for lambda excisive recombination. J Biol Chem. 1982 Aug 25;257(16):9658–9662. [PubMed] [Google Scholar]
  3. Abremski K., Hoess R. Bacteriophage P1 site-specific recombination. Purification and properties of the Cre recombinase protein. J Biol Chem. 1984 Feb 10;259(3):1509–1514. [PubMed] [Google Scholar]
  4. Abremski K., Hoess R. Phage P1 Cre-loxP site-specific recombination. Effects of DNA supercoiling on catenation and knotting of recombinant products. J Mol Biol. 1985 Jul 20;184(2):211–220. doi: 10.1016/0022-2836(85)90374-2. [DOI] [PubMed] [Google Scholar]
  5. Abremski K., Hoess R., Sternberg N. Studies on the properties of P1 site-specific recombination: evidence for topologically unlinked products following recombination. Cell. 1983 Apr;32(4):1301–1311. doi: 10.1016/0092-8674(83)90311-2. [DOI] [PubMed] [Google Scholar]
  6. Andrews B. J., Proteau G. A., Beatty L. G., Sadowski P. D. The FLP recombinase of the 2 micron circle DNA of yeast: interaction with its target sequences. Cell. 1985 Apr;40(4):795–803. doi: 10.1016/0092-8674(85)90339-3. [DOI] [PubMed] [Google Scholar]
  7. Arthur A., Sherratt D. Dissection of the transposition process: a transposon-encoded site-specific recombination system. Mol Gen Genet. 1979 Oct 1;175(3):267–274. doi: 10.1007/BF00397226. [DOI] [PubMed] [Google Scholar]
  8. Austin S., Ziese M., Sternberg N. A novel role for site-specific recombination in maintenance of bacterial replicons. Cell. 1981 Sep;25(3):729–736. doi: 10.1016/0092-8674(81)90180-x. [DOI] [PubMed] [Google Scholar]
  9. Babineau D., Vetter D., Andrews B. J., Gronostajski R. M., Proteau G. A., Beatty L. G., Sadowski P. D. The FLP protein of the 2-micron plasmid of yeast. Purification of the protein from Escherichia coli cells expressing the cloned FLP gene. J Biol Chem. 1985 Oct 5;260(22):12313–12319. [PubMed] [Google Scholar]
  10. Benjamin H. W., Matzuk M. M., Krasnow M. A., Cozzarelli N. R. Recombination site selection by Tn3 resolvase: topological tests of a tracking mechanism. Cell. 1985 Jan;40(1):147–158. doi: 10.1016/0092-8674(85)90318-6. [DOI] [PubMed] [Google Scholar]
  11. Bernard O., Hozumi N., Tonegawa S. Sequences of mouse immunoglobulin light chain genes before and after somatic changes. Cell. 1978 Dec;15(4):1133–1144. doi: 10.1016/0092-8674(78)90041-7. [DOI] [PubMed] [Google Scholar]
  12. Better M., Lu C., Williams R. C., Echols H. Site-specific DNA condensation and pairing mediated by the int protein of bacteriophage lambda. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5837–5841. doi: 10.1073/pnas.79.19.5837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Broach J. R., Guarascio V. R., Jayaram M. Recombination within the yeast plasmid 2mu circle is site-specific. Cell. 1982 May;29(1):227–234. doi: 10.1016/0092-8674(82)90107-6. [DOI] [PubMed] [Google Scholar]
  14. Campbell A. Some general questions about movable elements and their implications. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):1–9. doi: 10.1101/sqb.1981.045.01.003. [DOI] [PubMed] [Google Scholar]
  15. Clark S. P., Yoshikai Y., Taylor S., Siu G., Hood L., Mak T. W. Identification of a diversity segment of human T-cell receptor beta-chain, and comparison with the analogous murine element. 1984 Sep 27-Oct 3Nature. 311(5984):387–389. doi: 10.1038/311387a0. [DOI] [PubMed] [Google Scholar]
  16. Cozzarelli N. R., Krasnow M. A., Gerrard S. P., White J. H. A topological treatment of recombination and topoisomerases. Cold Spring Harb Symp Quant Biol. 1984;49:383–400. doi: 10.1101/sqb.1984.049.01.045. [DOI] [PubMed] [Google Scholar]
  17. Craig N. L., Nash H. A. The mechanism of phage lambda site-specific recombination: site-specific breakage of DNA by Int topoisomerase. Cell. 1983 Dec;35(3 Pt 2):795–803. doi: 10.1016/0092-8674(83)90112-5. [DOI] [PubMed] [Google Scholar]
  18. D'Agostaro G., Hevia E., Wu G. E., Murialdo H. Site-directed cleavage of immunoglobulin gene segments by lymphoid cell extracts. Can J Biochem Cell Biol. 1985 Sep;63(9):969–976. doi: 10.1139/o85-119. [DOI] [PubMed] [Google Scholar]
  19. Early P., Huang H., Davis M., Calame K., Hood L. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH. Cell. 1980 Apr;19(4):981–992. doi: 10.1016/0092-8674(80)90089-6. [DOI] [PubMed] [Google Scholar]
  20. Gascoigne N. R., Chien Y., Becker D. M., Kavaler J., Davis M. M. Genomic organization and sequence of T-cell receptor beta-chain constant- and joining-region genes. Nature. 1984 Aug 2;310(5976):387–391. doi: 10.1038/310387a0. [DOI] [PubMed] [Google Scholar]
  21. Gellert M. DNA topoisomerases. Annu Rev Biochem. 1981;50:879–910. doi: 10.1146/annurev.bi.50.070181.004311. [DOI] [PubMed] [Google Scholar]
  22. Golden J. W., Robinson S. J., Haselkorn R. Rearrangement of nitrogen fixation genes during heterocyst differentiation in the cyanobacterium Anabaena. Nature. 1985 Apr 4;314(6010):419–423. doi: 10.1038/314419a0. [DOI] [PubMed] [Google Scholar]
  23. Griffith J. D., Nash H. A. Genetic rearrangement of DNA induces knots with a unique topology: implications for the mechanism of synapsis and crossing-over. Proc Natl Acad Sci U S A. 1985 May;82(10):3124–3128. doi: 10.1073/pnas.82.10.3124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Grindley N. D., Lauth M. R., Wells R. G., Wityk R. J., Salvo J. J., Reed R. R. Transposon-mediated site-specific recombination: identification of three binding sites for resolvase at the res sites of gamma delta and Tn3. Cell. 1982 Aug;30(1):19–27. doi: 10.1016/0092-8674(82)90007-1. [DOI] [PubMed] [Google Scholar]
  25. Gronostajski R. M., Sadowski P. D. Determination of DNA sequences essential for FLP-mediated recombination by a novel method. J Biol Chem. 1985 Oct 5;260(22):12320–12327. [PubMed] [Google Scholar]
  26. Gronostajski R. M., Sadowski P. D. The FLP protein of the 2-micron plasmid of yeast. Inter- and intramolecular reactions. J Biol Chem. 1985 Oct 5;260(22):12328–12335. [PubMed] [Google Scholar]
  27. Gronostajski R. M., Sadowski P. D. The FLP recombinase of the Saccharomyces cerevisiae 2 microns plasmid attaches covalently to DNA via a phosphotyrosyl linkage. Mol Cell Biol. 1985 Nov;5(11):3274–3279. doi: 10.1128/mcb.5.11.3274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Guarneros G., Echols H. New mutants of bacteriophage lambda with a specific defect in excision from the host chromosome. J Mol Biol. 1970 Feb 14;47(3):565–574. doi: 10.1016/0022-2836(70)90323-2. [DOI] [PubMed] [Google Scholar]
  29. Hayday A. C., Saito H., Gillies S. D., Kranz D. M., Tanigawa G., Eisen H. N., Tonegawa S. Structure, organization, and somatic rearrangement of T cell gamma genes. Cell. 1985 Feb;40(2):259–269. doi: 10.1016/0092-8674(85)90140-0. [DOI] [PubMed] [Google Scholar]
  30. Hoess R. H., Abremski K. Interaction of the bacteriophage P1 recombinase Cre with the recombining site loxP. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1026–1029. doi: 10.1073/pnas.81.4.1026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Hoess R. H., Abremski K. Mechanism of strand cleavage and exchange in the Cre-lox site-specific recombination system. J Mol Biol. 1985 Feb 5;181(3):351–362. doi: 10.1016/0022-2836(85)90224-4. [DOI] [PubMed] [Google Scholar]
  32. Hoess R., Abremski K., Sternberg N. The nature of the interaction of the P1 recombinase Cre with the recombining site loxP. Cold Spring Harb Symp Quant Biol. 1984;49:761–768. doi: 10.1101/sqb.1984.049.01.086. [DOI] [PubMed] [Google Scholar]
  33. Hsu P. L., Landy A. Resolution of synthetic att-site Holliday structures by the integrase protein of bacteriophage lambda. Nature. 1984 Oct 25;311(5988):721–726. doi: 10.1038/311721a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Jayaram M. Two-micrometer circle site-specific recombination: the minimal substrate and the possible role of flanking sequences. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5875–5879. doi: 10.1073/pnas.82.17.5875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Johnson R. C., Bruist M. B., Glaccum M. B., Simon M. I. In vitro analysis of Hin-mediated site-specific recombination. Cold Spring Harb Symp Quant Biol. 1984;49:751–760. doi: 10.1101/sqb.1984.049.01.085. [DOI] [PubMed] [Google Scholar]
  36. Johnson R. C., Simon M. I. Hin-mediated site-specific recombination requires two 26 bp recombination sites and a 60 bp recombinational enhancer. Cell. 1985 Jul;41(3):781–791. doi: 10.1016/s0092-8674(85)80059-3. [DOI] [PubMed] [Google Scholar]
  37. Kahmann R., Rudt F., Koch C., Mertens G. G inversion in bacteriophage Mu DNA is stimulated by a site within the invertase gene and a host factor. Cell. 1985 Jul;41(3):771–780. doi: 10.1016/s0092-8674(85)80058-1. [DOI] [PubMed] [Google Scholar]
  38. Kaiser A. D., Masuda T. Evidence for a prophage excision gene in lambda. J Mol Biol. 1970 Feb 14;47(3):557–564. doi: 10.1016/0022-2836(70)90322-0. [DOI] [PubMed] [Google Scholar]
  39. Kamp D., Kahmann R., Zipser D., Broker T. R., Chow L. T. Inversion of the G DNA segment of phage Mu controls phage infectivity. Nature. 1978 Feb 9;271(5645):577–580. doi: 10.1038/271577a0. [DOI] [PubMed] [Google Scholar]
  40. Kikuchi Y., Nash H. A. Nicking-closing activity associated with bacteriophage lambda int gene product. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3760–3764. doi: 10.1073/pnas.76.8.3760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Krasnow M. A., Cozzarelli N. R. Site-specific relaxation and recombination by the Tn3 resolvase: recognition of the DNA path between oriented res sites. Cell. 1983 Apr;32(4):1313–1324. doi: 10.1016/0092-8674(83)90312-4. [DOI] [PubMed] [Google Scholar]
  42. Krasnow M. A., Stasiak A., Spengler S. J., Dean F., Koller T., Cozzarelli N. R. Determination of the absolute handedness of knots and catenanes of DNA. Nature. 1983 Aug 11;304(5926):559–560. doi: 10.1038/304559a0. [DOI] [PubMed] [Google Scholar]
  43. Mertens G., Hoffmann A., Blöcker H., Frank R., Kahmann R. Gin-mediated site-specific recombination in bacteriophage Mu DNA: overproduction of the protein and inversion in vitro. EMBO J. 1984 Oct;3(10):2415–2421. doi: 10.1002/j.1460-2075.1984.tb02148.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Meyer-Leon L., Senecoff J. F., Bruckner R. C., Cox M. M. Site-specific genetic recombination promoted by the FLP protein of the yeast 2-micron plasmid in vitro. Cold Spring Harb Symp Quant Biol. 1984;49:797–804. doi: 10.1101/sqb.1984.049.01.090. [DOI] [PubMed] [Google Scholar]
  45. Mizuuchi K., Gellert M., Nash H. A. Involement of supertwisted DNA in integrative recombination of bacteriophage lambda. J Mol Biol. 1978 May 25;121(3):375–392. doi: 10.1016/0022-2836(78)90370-4. [DOI] [PubMed] [Google Scholar]
  46. Mizuuchi K., Gellert M., Weisberg R. A., Nash H. A. Catenation and supercoiling in the products of bacteriophage lambda integrative recombination in vitro. J Mol Biol. 1980 Aug 25;141(4):485–494. doi: 10.1016/0022-2836(80)90256-9. [DOI] [PubMed] [Google Scholar]
  47. Mizuuchi K., Mizuuchi M. Integrative recombination of bacteriophage lambda: in vitro study of the intermolecular reaction. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1111–1114. doi: 10.1101/sqb.1979.043.01.123. [DOI] [PubMed] [Google Scholar]
  48. Mizuuchi K., Weisberg R., Enquist L., Mizuuchi M., Buraczynska M., Foeller C., Hsu P. L., Ross W., Landy A. Structure and function of the phage lambda att site: size, int-binding sites, and location of the crossover point. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):429–437. doi: 10.1101/sqb.1981.045.01.057. [DOI] [PubMed] [Google Scholar]
  49. Nash H. A. Integrative recombination of bacteriophage lambda DNA in vitro. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1072–1076. doi: 10.1073/pnas.72.3.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  50. Nash H. A., Mizuuchi K., Enquist L. W., Weisberg R. A. Strand exchange in lambda integrative recombination: genetics, biochemistry, and models. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):417–428. doi: 10.1101/sqb.1981.045.01.056. [DOI] [PubMed] [Google Scholar]
  51. Nash H. A., Pollock T. J. Site-specific recombination of bacteriophage lambda. The change in topological linking number associated with exchange of DNA strands. J Mol Biol. 1983 Oct 15;170(1):19–38. doi: 10.1016/s0022-2836(83)80225-3. [DOI] [PubMed] [Google Scholar]
  52. Nash H. A., Robertson C. A. Purification and properties of the Escherichia coli protein factor required for lambda integrative recombination. J Biol Chem. 1981 Sep 10;256(17):9246–9253. [PubMed] [Google Scholar]
  53. Newman B. J., Grindley N. D. Mutants of the gamma delta resolvase: a genetic analysis of the recombination function. Cell. 1984 Sep;38(2):463–469. doi: 10.1016/0092-8674(84)90501-4. [DOI] [PubMed] [Google Scholar]
  54. Plasterk R. H., Kanaar R., van de Putte P. A genetic switch in vitro: DNA inversion by Gin protein of phage Mu. Proc Natl Acad Sci U S A. 1984 May;81(9):2689–2692. doi: 10.1073/pnas.81.9.2689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  55. Pollock T. J., Nash H. A. Knotting of DNA caused by a genetic rearrangement. Evidence for a nucleosome-like structure in site-specific recombination of bacteriophage lambda. J Mol Biol. 1983 Oct 15;170(1):1–18. doi: 10.1016/s0022-2836(83)80224-1. [DOI] [PubMed] [Google Scholar]
  56. Reed R. R., Grindley N. D. Transposon-mediated site-specific recombination in vitro: DNA cleavage and protein-DNA linkage at the recombination site. Cell. 1981 Sep;25(3):721–728. doi: 10.1016/0092-8674(81)90179-3. [DOI] [PubMed] [Google Scholar]
  57. Reed R. R., Moser C. D. Resolvase-mediated recombination intermediates contain a serine residue covalently linked to DNA. Cold Spring Harb Symp Quant Biol. 1984;49:245–249. doi: 10.1101/sqb.1984.049.01.028. [DOI] [PubMed] [Google Scholar]
  58. Reed R. R. Transposon-mediated site-specific recombination: a defined in vitro system. Cell. 1981 Sep;25(3):713–719. doi: 10.1016/0092-8674(81)90178-1. [DOI] [PubMed] [Google Scholar]
  59. Shapiro J. A. Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1933–1937. doi: 10.1073/pnas.76.4.1933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  60. Sherratt D., Dyson P., Boocock M., Brown L., Summers D., Stewart G., Chan P. Site-specific recombination in transposition and plasmid stability. Cold Spring Harb Symp Quant Biol. 1984;49:227–233. doi: 10.1101/sqb.1984.049.01.026. [DOI] [PubMed] [Google Scholar]
  61. Shulman M., Gottesman M. Attachment site mutants of bacteriophage lambda. J Mol Biol. 1973 Dec 25;81(4):461–482. doi: 10.1016/0022-2836(73)90517-2. [DOI] [PubMed] [Google Scholar]
  62. Simon M., Zieg J., Silverman M., Mandel G., Doolittle R. Phase variation: evolution of a controlling element. Science. 1980 Sep 19;209(4463):1370–1374. doi: 10.1126/science.6251543. [DOI] [PubMed] [Google Scholar]
  63. Spengler S. J., Stasiak A., Cozzarelli N. R. The stereostructure of knots and catenanes produced by phage lambda integrative recombination: implications for mechanism and DNA structure. Cell. 1985 Aug;42(1):325–334. doi: 10.1016/s0092-8674(85)80128-8. [DOI] [PubMed] [Google Scholar]
  64. Sternberg N., Hamilton D., Hoess R. Bacteriophage P1 site-specific recombination. II. Recombination between loxP and the bacterial chromosome. J Mol Biol. 1981 Aug 25;150(4):487–507. doi: 10.1016/0022-2836(81)90376-4. [DOI] [PubMed] [Google Scholar]
  65. Vetter D., Andrews B. J., Roberts-Beatty L., Sadowski P. D. Site-specific recombination of yeast 2-micron DNA in vitro. Proc Natl Acad Sci U S A. 1983 Dec;80(23):7284–7288. doi: 10.1073/pnas.80.23.7284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  66. Wasserman S. A., Cozzarelli N. R. Determination of the stereostructure of the product of Tn3 resolvase by a general method. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1079–1083. doi: 10.1073/pnas.82.4.1079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  67. Wasserman S. A., Dungan J. M., Cozzarelli N. R. Discovery of a predicted DNA knot substantiates a model for site-specific recombination. Science. 1985 Jul 12;229(4709):171–174. doi: 10.1126/science.2990045. [DOI] [PubMed] [Google Scholar]
  68. Yin S., Bushman W., Landy A. Interaction of the lambda site-specific recombination protein Xis with attachment site DNA. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1040–1044. doi: 10.1073/pnas.82.4.1040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  69. de Massy B., Studier F. W., Dorgai L., Appelbaum E., Weisberg R. A. Enzymes and sites of genetic recombination: studies with gene-3 endonuclease of phage T7 and with site-affinity mutants of phage lambda. Cold Spring Harb Symp Quant Biol. 1984;49:715–726. doi: 10.1101/sqb.1984.049.01.081. [DOI] [PubMed] [Google Scholar]
  70. van de Putte P., Cramer S., Giphart-Gassler M. Invertible DNA determines host specificity of bacteriophage mu. Nature. 1980 Jul 17;286(5770):218–222. doi: 10.1038/286218a0. [DOI] [PubMed] [Google Scholar]

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