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. 1986 Feb;165(2):373–378. doi: 10.1128/jb.165.2.373-378.1986

Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning of mec-specific DNA.

W D Beck, B Berger-Bächi, F H Kayser
PMCID: PMC214427  PMID: 3003024

Abstract

Additional DNA was shown to be present in methicillin-resistant Staphylococcus aureus by one- and two-dimensional restriction endonuclease analyses of the chromosomal DNA. A 3.5-kilobase Bg/II fragment, which was present in methicillin-resistant strains but not in the isogenic methicillin-sensitive parental strain, was cloned into newly constructed plasmid pWDB1 in Escherichia coli. Hybridization of this 3.5-kilobase Bg/II fragment with different methicillin-sensitive and methicillin-resistant S. aureus clinical isolates indicated that the fragment represents part of the methicillin resistance determinant (mec). In addition, the fragment carries a sequence that is present in some large staphylococcal plasmids, as well as in penicillinase plasmid pI524.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berger-Bächi B. Increase in transduction efficiency of Tn551 mediated by the methicillin resistance marker. J Bacteriol. 1983 Apr;154(1):533–535. doi: 10.1128/jb.154.1.533-535.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berger-Bächi B. Insertional inactivation of staphylococcal methicillin resistance by Tn551. J Bacteriol. 1983 Apr;154(1):479–487. doi: 10.1128/jb.154.1.479-487.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bächi B. Physical mapping of the BglI, BglII, PstI and EcoRI restriction fragments of staphylococcal phage phi 11 DNA. Mol Gen Genet. 1980;180(2):391–398. doi: 10.1007/BF00425853. [DOI] [PubMed] [Google Scholar]
  4. Cohen S., Sweeney H. M., Basu S. K. Mutations in prophage phi11 that impair the transducibility of their Staphylococcus aureus lysogens for methicillin resistance. J Bacteriol. 1977 Jan;129(1):237–245. doi: 10.1128/jb.129.1.237-245.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hohn B., Collins J. A small cosmid for efficient cloning of large DNA fragments. Gene. 1980 Nov;11(3-4):291–298. doi: 10.1016/0378-1119(80)90069-4. [DOI] [PubMed] [Google Scholar]
  6. Hoopes B. C., McClure W. R. Studies on the selectivity of DNA precipitation by spermine. Nucleic Acids Res. 1981 Oct 24;9(20):5493–5504. doi: 10.1093/nar/9.20.5493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kayser F. H., Benner E. J., Troy R., Hoeprich P. D. Experimental and clinical aspects of resistance determinants. Mode of resistance against beta-lactam antibiotics in staphylococci. Ann N Y Acad Sci. 1971 Jun 11;182:106–117. doi: 10.1111/j.1749-6632.1971.tb30649.x. [DOI] [PubMed] [Google Scholar]
  8. Kayser F. H. Die Resistenz methicillinresistenter Staphylokokken gegenüber neuen Cephalosporin-Antibiotika. Infection. 1980;8(4):165–170. doi: 10.1007/BF01639125. [DOI] [PubMed] [Google Scholar]
  9. Kieser T. Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid. 1984 Jul;12(1):19–36. doi: 10.1016/0147-619x(84)90063-5. [DOI] [PubMed] [Google Scholar]
  10. Kuhl S. A., Pattee P. A., Baldwin J. N. Chromosomal map location of the methicillin resistance determinant in Staphylococcus aureus. J Bacteriol. 1978 Aug;135(2):460–465. doi: 10.1128/jb.135.2.460-465.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lacey R. W. Genetic control in methicillin-resistant strains of Staphylococcus aureus. J Med Microbiol. 1972 Nov;5(4):497–508. doi: 10.1099/00222615-5-4-497. [DOI] [PubMed] [Google Scholar]
  12. Murphy E., Novick R. P. Physical mapping of Staphylococcus aureus penicillinase plasmid pI524: characterization of an invertible region. Mol Gen Genet. 1979 Aug;175(1):19–30. doi: 10.1007/BF00267851. [DOI] [PubMed] [Google Scholar]
  13. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  14. Shafer W. M., Iandolo J. J. Genetics of staphylococcal enterotoxin B in methicillin-resistant isolates of Staphylococcus aureus. Infect Immun. 1979 Sep;25(3):902–911. doi: 10.1128/iai.25.3.902-911.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  16. Stewart G. C., Rosenblum E. D. Genetic behavior of the methicillin resistance determinant in Staphylococcus aureus. J Bacteriol. 1980 Dec;144(3):1200–1202. doi: 10.1128/jb.144.3.1200-1202.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yee T., Inouye M. Two-dimensional DNA electrophoresis applied to the study of DNA methylation and the analysis of genome size in Myxococcus xanthus. J Mol Biol. 1982 Jan 15;154(2):181–196. doi: 10.1016/0022-2836(82)90059-6. [DOI] [PubMed] [Google Scholar]

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