Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1986 Feb;165(2):420–423. doi: 10.1128/jb.165.2.420-423.1986

Stage-specific DNA methylation in a fungal plant pathogen.

E R Jupe, J M Magill, C W Magill
PMCID: PMC214434  PMID: 3003026

Abstract

Significantly more 5-methylcytosine residues were found in the DNA from the dormant sclerotia of Phymatotrichum omnivorum than in the DNA from the metabolically active mycelia of the fungus, as shown by high-pressure liquid chromatography of acid-hydrolyzed DNA digests and by restriction of the DNA with the isoschizomers MspI and HpaII. N6-Methyladenine was not detected in GATC sequences in the DNA isolated from either stage.

Full text

PDF
420

Images in this article

421Image
on p.421
421Image
on p.421
422Image
on p.422

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antequera F., Tamame M., Villanueva J. R., Santos T. DNA methylation in the fungi. J Biol Chem. 1984 Jul 10;259(13):8033–8036. [PubMed] [Google Scholar]
  2. Bestor T. H., Hellewell S. B., Ingram V. M. Differentiation of two mouse cell lines is associated with hypomethylation of their genomes. Mol Cell Biol. 1984 Sep;4(9):1800–1806. doi: 10.1128/mcb.4.9.1800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blackburn E. H., Pan W. C., Johnson C. C. Methylation of ribosomal RNA genes in the macronucleus of Tetrahymena thermophila. Nucleic Acids Res. 1983 Aug 11;11(15):5131–5145. doi: 10.1093/nar/11.15.5131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bolen P. L., Grant D. M., Swinton D., Boynton J. E., Gillham N. W. Extensive methylation of chloroplast DNA by a nuclear gene mutation does not affect chloroplast gene transmission in chlamydomonas. Cell. 1982 Feb;28(2):335–343. doi: 10.1016/0092-8674(82)90351-8. [DOI] [PubMed] [Google Scholar]
  5. Bull J. H., Wootton J. C. Heavily methylated amplified DNA in transformants of Neurospora crassa. Nature. 1984 Aug 23;310(5979):701–704. doi: 10.1038/310701a0. [DOI] [PubMed] [Google Scholar]
  6. Charache S., Dover G., Smith K., Talbot C. C., Jr, Moyer M., Boyer S. Treatment of sickle cell anemia with 5-azacytidine results in increased fetal hemoglobin production and is associated with nonrandom hypomethylation of DNA around the gamma-delta-beta-globin gene complex. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4842–4846. doi: 10.1073/pnas.80.15.4842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cummings D. J., Tait A., Goddard J. M. Methylated bases in DNA from Paramecium aurelia. Biochim Biophys Acta. 1974 Nov 20;374(1):1–11. doi: 10.1016/0005-2787(74)90194-4. [DOI] [PubMed] [Google Scholar]
  8. Doerfler W. DNA methylation and gene activity. Annu Rev Biochem. 1983;52:93–124. doi: 10.1146/annurev.bi.52.070183.000521. [DOI] [PubMed] [Google Scholar]
  9. Feng T. Y., Chiang K. S. The persistence of maternal inheritance in Chlamydomonas despite hypomethylation of chloroplast DNA induced by inhibitors. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3438–3442. doi: 10.1073/pnas.81.11.3438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gorovsky M. A., Hattman S., Pleger G. L. ( 6 N)methyl adenine in the nuclear DNA of a eucaryote, Tetrahymena pyriformis. J Cell Biol. 1973 Mar;56(3):697–701. doi: 10.1083/jcb.56.3.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hattman S., Kenny C., Berger L., Pratt K. Comparative study of DNA methylation in three unicellular eucaryotes. J Bacteriol. 1978 Sep;135(3):1156–1157. doi: 10.1128/jb.135.3.1156-1157.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones P. A., Taylor S. M., Mohandas T., Shapiro L. J. Cell cycle-specific reactivation of an inactive X-chromosome locus by 5-azadeoxycytidine. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1215–1219. doi: 10.1073/pnas.79.4.1215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mavilio F., Giampaolo A., Carè A., Migliaccio G., Calandrini M., Russo G., Pagliardi G. L., Mastroberardino G., Marinucci M., Peschle C. Molecular mechanisms of human hemoglobin switching: selective undermethylation and expression of globin genes in embryonic, fetal, and adult erythroblasts. Proc Natl Acad Sci U S A. 1983 Nov;80(22):6907–6911. doi: 10.1073/pnas.80.22.6907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mohandas T., Sparkes R. S., Shapiro L. J. Reactivation of an inactive human X chromosome: evidence for X inactivation by DNA methylation. Science. 1981 Jan 23;211(4480):393–396. doi: 10.1126/science.6164095. [DOI] [PubMed] [Google Scholar]
  15. Peoples O. P., Hardman N. An abundant family of methylated repetitive sequences dominates the genome of Physarum polycephalum. Nucleic Acids Res. 1983 Nov 25;11(22):7777–7788. doi: 10.1093/nar/11.22.7777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pratt K., Hattman S. Deoxyribonucleic acid methylation and chromatin organization in Tetrahymena thermophila. Mol Cell Biol. 1981 Jul;1(7):600–608. doi: 10.1128/mcb.1.7.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Proffitt J. H., Davie J. R., Swinton D., Hattman S. 5-Methylcytosine is not detectable in Saccharomyces cerevisiae DNA. Mol Cell Biol. 1984 May;4(5):985–988. doi: 10.1128/mcb.4.5.985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rae P. M., Steele R. E. Modified bases in the DNAs of unicellular eukaryotes: an examination of distributions and possible roles, with emphasis on hydroxymethyluracil in dinoflagellates. Biosystems. 1978 Apr;10(1-2):37–53. doi: 10.1016/0303-2647(78)90027-8. [DOI] [PubMed] [Google Scholar]
  19. Royer H. D., Sager R. Methylation of chloroplast DNAs in the life cycle of Chlamydomonas. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5794–5798. doi: 10.1073/pnas.76.11.5794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sager R., Grabowy C., Sano H. The mat-1 gene in Chlamydomonas regulates DNA methylation during gametogenesis. Cell. 1981 Apr;24(1):41–47. doi: 10.1016/0092-8674(81)90499-2. [DOI] [PubMed] [Google Scholar]
  21. Tamame M., Antequera F., Villanueva J. R., Santos T. High-frequency conversion to a "fluffy" developmental phenotype in Aspergillus spp. by 5-azacytidine treatment: evidence for involvement of a single nuclear gene. Mol Cell Biol. 1983 Dec;3(12):2287–2297. doi: 10.1128/mcb.3.12.2287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Urieli-Shoval S., Gruenbaum Y., Sedat J., Razin A. The absence of detectable methylated bases in Drosophila melanogaster DNA. FEBS Lett. 1982 Sep 6;146(1):148–152. doi: 10.1016/0014-5793(82)80723-0. [DOI] [PubMed] [Google Scholar]
  23. Van Etten J. L., Schuster A. M., Girton L., Burbank D. E., Swinton D., Hattman S. DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga. Nucleic Acids Res. 1985 May 24;13(10):3471–3478. doi: 10.1093/nar/13.10.3471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Whittaker P. A., Hardman N. Methylation of nuclear DNA in Physarum polycephalum. Biochem J. 1980 Dec 1;191(3):859–862. doi: 10.1042/bj1910859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wilson V. L., Jones P. A. Inhibition of DNA methylation by chemical carcinogens in vitro. Cell. 1983 Jan;32(1):239–246. doi: 10.1016/0092-8674(83)90514-7. [DOI] [PubMed] [Google Scholar]
  26. Wolf S. F., Jolly D. J., Lunnen K. D., Friedmann T., Migeon B. R. Methylation of the hypoxanthine phosphoribosyltransferase locus on the human X chromosome: implications for X-chromosome inactivation. Proc Natl Acad Sci U S A. 1984 May;81(9):2806–2810. doi: 10.1073/pnas.81.9.2806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wolf S. F., Migeon B. R. Clusters of CpG dinucleotides implicated by nuclease hypersensitivity as control elements of housekeeping genes. Nature. 1985 Apr 4;314(6010):467–469. doi: 10.1038/314467a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES