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. 1986 Feb;165(2):448–452. doi: 10.1128/jb.165.2.448-452.1986

Specialized transduction of Pseudomonas aeruginosa PAO by bacteriophage D3.

M M Cavenagh, R V Miller
PMCID: PMC214439  PMID: 3080405

Abstract

D3, a temperate bacteriophage of Pseudomonas aeruginosa PAO, was found to specifically transduce the alleles met-49 and met-117. Induction of established lysogens with UV light was necessary for the production of transducing lysates. Transduced cells were immune to superinfection by phage D3 and could give rise to high-frequency transducing lysates. Cotransduction of these two alleles could not be demonstrated. met-117 was mapped to 26 min on the PAO genetic map. Complementation studies using the generalized transducing phage F116L indicated that met-49 is an allele of met-9011 which maps at 55 min. The integrated D3 prophage was shown to be coinherited with met-117 and with met-49.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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