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. Author manuscript; available in PMC: 2007 Dec 18.
Published in final edited form as: Nature. 2007 May 9;447(7144):556–561. doi: 10.1038/nature05848

Figure 2.

Figure 2

Analysis of CaNM recognition sequences and the species barrier between Sc/Ca NM. a, Quantification of the fluorescence of labelled full-length CaNM bound to overlapping 20mer CaNM peptides after two hours (1 μM, 5% Alexa Fluor 647) and two and a half days (inset, 5 μM, 75% Alexa Fluor 647). b, Quantification of the fluorescence of labelled full-length ScNM (1 μM, 5% Alexa Fluor 555) bound to overlapping 20mer CaNM peptides after two hours. c, Quantification of the fluorescence of labelled full-length CaNM (1 μM, 5% Alexa Fluor 647) bound to overlapping 20mer ScNM peptides after two hours. d, Image of a single peptide array that was simultaneously incubated with labelled full-length ScNM (green, Alexa Fluor 555) and CaNM (red, Alexa Fluor 647) for two hours (1 μM final concentration of each protein, 5% label). e, Quantification of the fluorescence of labelled full-length Sc/Ca NM chimera (1 μM NM, 5% Alexa Fluor 647) bound to both ScNM and CaNM peptides after two hours of incubation.

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