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. 1986 Mar;165(3):1015–1018. doi: 10.1128/jb.165.3.1015-1018.1986

Transformation of Bacillus subtilis by single-stranded plasmid DNA.

C F Rudolph, B J Schmidt, C W Saunders
PMCID: PMC214530  PMID: 3081487

Abstract

The single-stranded form of a pE194-based plasmid transformed Bacillus subtilis protoplasts at least as efficiently as did the double-stranded plasmid, but the single-stranded form did not detectably transform B. subtilis competent cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barany F., Boeke J. D. Genetic transformation of Streptococcus pneumoniae by DNA cloned into the single-stranded bacteriophage f1. J Bacteriol. 1983 Jan;153(1):200–210. doi: 10.1128/jb.153.1.200-210.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benzinger R. Transfection of Enterobacteriaceae and its applications. Microbiol Rev. 1978 Mar;42(1):194–236. doi: 10.1128/mr.42.1.194-236.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  4. Canosi U., Morelli G., Trautner T. A. The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis. Mol Gen Genet. 1978 Nov 9;166(3):259–267. doi: 10.1007/BF00267617. [DOI] [PubMed] [Google Scholar]
  5. Chang S., Cohen S. N. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet. 1979 Jan 5;168(1):111–115. doi: 10.1007/BF00267940. [DOI] [PubMed] [Google Scholar]
  6. Chilton M. D., Hall B. D. Transforming activity in single-stranded DNA from Bacillus subtilis. J Mol Biol. 1968 Jun 28;34(3):439–451. doi: 10.1016/0022-2836(68)90171-x. [DOI] [PubMed] [Google Scholar]
  7. Chilton M. D. Transforming Activity in Both Complementary Strands of Bacillus subtilis DNA. Science. 1967 Aug 18;157(3790):817–819. doi: 10.1126/science.157.3790.817. [DOI] [PubMed] [Google Scholar]
  8. Dotto G. P., Horiuchi K. Replication of a plasmid containing two origins of bacteriophage. J Mol Biol. 1981 Nov 25;153(1):169–176. doi: 10.1016/0022-2836(81)90532-5. [DOI] [PubMed] [Google Scholar]
  9. Ehrlich S. D. Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1680–1682. doi: 10.1073/pnas.74.4.1680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gingeras T. R., Greenough L., Schildkraut I., Roberts R. J. Two new restriction endonucleases from Proteus vulgaris. Nucleic Acids Res. 1981 Sep 25;9(18):4525–4536. doi: 10.1093/nar/9.18.4525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kalnins A., Otto K., Rüther U., Müller-Hill B. Sequence of the lacZ gene of Escherichia coli. EMBO J. 1983;2(4):593–597. doi: 10.1002/j.1460-2075.1983.tb01468.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lovett P. S., Duvall E. J., Keggins K. M. Bacillus pumilus plasmid pPL10: properties and insertion into Bacillus subtilis 168 by transformation. J Bacteriol. 1976 Aug;127(2):817–828. doi: 10.1128/jb.127.2.817-828.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lévi-Meyrueis C., Fodor K., Schaeffer P. Polyethyleneglycol-induced transformation of Bacillus subtilis protoplasts by bacterial chromosomal DNA. Mol Gen Genet. 1980;179(3):589–594. doi: 10.1007/BF00271749. [DOI] [PubMed] [Google Scholar]
  14. MIYAZAWA Y., THOMAS C. A., Jr NUCLEOTIDE COMPOSITION OF SHORT SEGMENTS OF DNA MOLECULES. J Mol Biol. 1965 Feb;11:223–237. doi: 10.1016/s0022-2836(65)80053-5. [DOI] [PubMed] [Google Scholar]
  15. Mottes M., Grandi G., Sgaramella V., Canosi U., Morelli G., Trautner T. A. Different specific activities of the monomeric and oligomeric forms of plasmid DNA in transformation of B. subtilis and E. coli. Mol Gen Genet. 1979 Jul 24;174(3):281–286. doi: 10.1007/BF00267800. [DOI] [PubMed] [Google Scholar]
  16. Saunders C. W., Schmidt B. J., Mirot M. S., Thompson L. D., Guyer M. S. Use of chromosomal integration in the establishment and expression of blaZ, a Staphylococcus aureus beta-lactamase gene, in Bacillus subtilis. J Bacteriol. 1984 Mar;157(3):718–726. doi: 10.1128/jb.157.3.718-726.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sonenshein A. L., Cami B., Brevet J., Cote R. Isolation and characterization of rifampin-resistant and streptolydigin-resistant mutants of Bacillus subtilis with altered sporulation properties. J Bacteriol. 1974 Oct;120(1):253–265. doi: 10.1128/jb.120.1.253-265.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sutcliffe J. G. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737–3741. doi: 10.1073/pnas.75.8.3737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vasantha N., Thompson L. D., Rhodes C., Banner C., Nagle J., Filpula D. Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein. J Bacteriol. 1984 Sep;159(3):811–819. doi: 10.1128/jb.159.3.811-819.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. de Vos W. M., Venema G., Canosi U., Trautner T. A. Plasmid transformation in Bacillus subtilis: fate of plasmid DNA. Mol Gen Genet. 1981;181(4):424–433. doi: 10.1007/BF00428731. [DOI] [PubMed] [Google Scholar]
  21. de Vos W. M., Venema G. Fate of plasmid DNA in transformation of Bacillus subtilis protoplasts. Mol Gen Genet. 1981;182(1):39–43. doi: 10.1007/BF00422764. [DOI] [PubMed] [Google Scholar]

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