Abstract
From a partial Sau3A gene library of Bacillus subtilis chromosomal DNA in the expression plasmid pRK9, four hybrid plasmids were isolated carrying overlapping segments of the argA-argF-cpa cluster. The complementation patterns within Escherichia coli arginine auxotrophs of these hybrids and deletion derivatives provided the gene order argC-argA-argE-argB-argD-cpa-argF.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baumberg S., Harwood C. R. Carbon and nitrogen repression of arginine catabolic enzymes in Bacillus subtilis. J Bacteriol. 1979 Jan;137(1):189–196. doi: 10.1128/jb.137.1.189-196.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baumberg S., Mountain A. Bacillus subtilis 168 mutants resistant to arginine hydroxamate in the presence of ornithine or citrulline. J Gen Microbiol. 1984 May;130(5):1247–1252. doi: 10.1099/00221287-130-5-1247. [DOI] [PubMed] [Google Scholar]
- Harwood C. R., Baumberg S. Arginine hydroxamate-resistant mutants of Bacillus subtilis with altered control of arginine metabolism. J Gen Microbiol. 1977 May;100(1):177–188. doi: 10.1099/00221287-100-1-177. [DOI] [PubMed] [Google Scholar]
- Henner D. J., Hoch J. A. The Bacillus subtilis chromosome. Microbiol Rev. 1980 Mar;44(1):57–82. doi: 10.1128/mr.44.1.57-82.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAHLER I., NEUMANN I. M., MARMUR J. Studies of genetic-units controlling arginine biosynthesis in Bacillus subtilis. Biochim Biophys Acta. 1963 May 28;72:69–79. [PubMed] [Google Scholar]
- Mann N. H., Mountain A., Munton R. N., Smith M. C., Baumberg S. Transcription analysis of a Bacillus subtilis arg gene following cloning in Escherichia coli in an initially unstable hybrid plasmid. Mol Gen Genet. 1984;197(1):75–81. doi: 10.1007/BF00327925. [DOI] [PubMed] [Google Scholar]
- Miozzari G. F., Yanofsky C. The regulatory region of the trp operon of Serratia marcescens. Nature. 1978 Dec 14;276(5689):684–689. doi: 10.1038/276684a0. [DOI] [PubMed] [Google Scholar]
- Mountain A., Baumberg S. Map locations of some mutations conferring resistance to arginine hydroxamate in Bacillus subtilis 168. Mol Gen Genet. 1980;178(3):691–701. doi: 10.1007/BF00337880. [DOI] [PubMed] [Google Scholar]
- Mountain A., Mann N. H., Munton R. N., Baumberg S. Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis. Mol Gen Genet. 1984;197(1):82–89. doi: 10.1007/BF00327926. [DOI] [PubMed] [Google Scholar]
- Nichols B. P., Yanofsky C. Plasmids containing the trp promoters of Escherichia coli and Serratia marcescens and their use in expressing cloned genes. Methods Enzymol. 1983;101:155–164. doi: 10.1016/0076-6879(83)01011-3. [DOI] [PubMed] [Google Scholar]
- Nyunoya H., Lusty C. J. The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4629–4633. doi: 10.1073/pnas.80.15.4629. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paulus T. J., Switzer R. L. Characterization of pyrimidine-repressible and arginine-repressible carbamyl phosphate synthetases from Bacillus subtilis. J Bacteriol. 1979 Jan;137(1):82–91. doi: 10.1128/jb.137.1.82-91.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Potvin B., Gooder H. Carbamyl phosphate synthesis in Bacillus subtilis. Biochem Genet. 1975 Feb;13(1-2):125–143. doi: 10.1007/BF00486011. [DOI] [PubMed] [Google Scholar]
- Schechtman M. G., Yanofsky C. Structure of the trifunctional trp-1 gene from Neurospora crassa and its aberrant expression in Escherichia coli. J Mol Appl Genet. 1983;2(1):83–99. [PubMed] [Google Scholar]