Abstract
The nucleotide sequence of the DNA region involved in partitioning of plasmid mini-F has been determined. The sequence consists of 12 direct tandemly arranged repeats of 43 base pairs (the two flanking repeats, 43 plus 1 base pairs) with extensive homology to each other. Each repeat contains an additional inverted repeat of 7 base pairs.
Full text
PDF![1043](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f242/214538/6efa37062e2b/jbacter00214-0389.png)
![1044](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f242/214538/8ad287f0ed2f/jbacter00214-0390.png)
![1045](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f242/214538/908af167fdd4/jbacter00214-0391.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Austin S., Abeles A. Partition of unit-copy miniplasmids to daughter cells. II. The partition region of miniplasmid P1 encodes an essential protein and a centromere-like site at which it acts. J Mol Biol. 1983 Sep 15;169(2):373–387. doi: 10.1016/s0022-2836(83)80056-4. [DOI] [PubMed] [Google Scholar]
- Austin S., Wierzbicki A. Two mini-F-encoded proteins are essential for equipartition. Plasmid. 1983 Jul;10(1):73–81. doi: 10.1016/0147-619x(83)90059-8. [DOI] [PubMed] [Google Scholar]
- Bergquist P. L., Lane H. E., Malcolm L., Downard R. A. Molecular homology and incompatibility in the IncFI plasmid Group. J Gen Microbiol. 1982 Feb;128(2):223–238. doi: 10.1099/00221287-128-2-223. [DOI] [PubMed] [Google Scholar]
- Deininger P. L. Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis. Anal Biochem. 1983 Feb 15;129(1):216–223. doi: 10.1016/0003-2697(83)90072-6. [DOI] [PubMed] [Google Scholar]
- Gardner R. C., Malcolm L., Bergquist P. L., Lane H. E. IncD, a genetic locus in F responsible for incompatibility with several plasmids of the IncFI group. Mol Gen Genet. 1982;188(2):345–352. doi: 10.1007/BF00332699. [DOI] [PubMed] [Google Scholar]
- Hakkaart M. J., Veltkamp E., Nijkamp H. J. Maintenance of the bacteriocinogenic plasmid Clo DF13 in Escherichia coli cells. II. Specific recombination functions involved in plasmid maintenance. Mol Gen Genet. 1982;188(2):338–344. doi: 10.1007/BF00332698. [DOI] [PubMed] [Google Scholar]
- Hayakawa Y., Murotsu T., Matsubara K. Mini-F protein that binds to a unique region for partition of mini-F plasmid DNA. J Bacteriol. 1985 Jul;163(1):349–354. doi: 10.1128/jb.163.1.349-354.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kline B. C. Incompatibility between Flac, R386, and F:pSC101 recombinant plasmids: the specificity of F incompatibility genes. Plasmid. 1979 Jul;2(3):437–445. doi: 10.1016/0147-619x(79)90027-1. [DOI] [PubMed] [Google Scholar]
- Komai N., Nishizawa T., Hayakawa Y., Murotsu T., Matsubara K. Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments. Mol Gen Genet. 1982;186(2):193–203. doi: 10.1007/BF00331850. [DOI] [PubMed] [Google Scholar]
- Lane H. E. Replication and incompatibility of F and plasmids in the IncFI Group. Plasmid. 1981 Jan;5(1):100–126. doi: 10.1016/0147-619x(81)90079-2. [DOI] [PubMed] [Google Scholar]
- Messing J., Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. doi: 10.1016/0378-1119(82)90016-6. [DOI] [PubMed] [Google Scholar]
- Miki T., Easton A. M., Rownd R. H. Cloning of replication, incompatibility, and stability functions of R plasmid NR1. J Bacteriol. 1980 Jan;141(1):87–99. doi: 10.1128/jb.141.1.87-99.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murotsu T., Matsubara K., Sugisaki H., Takanami M. Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid. Gene. 1981 Nov;15(2-3):257–271. doi: 10.1016/0378-1119(81)90135-9. [DOI] [PubMed] [Google Scholar]
- Nordström K., Molin S., Aagaard-Hansen H. Partitioning of plasmid R1 in Escherichia coli. II. Incompatibility properties of the partitioning system. Plasmid. 1980 Nov;4(3):332–339. doi: 10.1016/0147-619x(80)90071-2. [DOI] [PubMed] [Google Scholar]
- Ogura T., Hiraga S. Partition mechanism of F plasmid: two plasmid gene-encoded products and a cis-acting region are involved in partition. Cell. 1983 Feb;32(2):351–360. doi: 10.1016/0092-8674(83)90454-3. [DOI] [PubMed] [Google Scholar]
- Phua S. H., Bergquist P. L., Lane H. E. Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis. Mol Gen Genet. 1982;188(2):353–355. doi: 10.1007/BF00332700. [DOI] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith H. O. Recovery of DNA from gels. Methods Enzymol. 1980;65(1):371–380. doi: 10.1016/s0076-6879(80)65048-4. [DOI] [PubMed] [Google Scholar]
- Wehlmann H., Eichenlaub R. Analysis of transcripts from plasmid mini-F by electron microscopy of R loops. Plasmid. 1981 May;5(3):259–266. doi: 10.1016/0147-619x(81)90003-2. [DOI] [PubMed] [Google Scholar]
- Wehlmann H., Eichenlaub R. Plasmid mini-F encoded proteins. Mol Gen Genet. 1980;180(1):205–211. doi: 10.1007/BF00267371. [DOI] [PubMed] [Google Scholar]