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. 1986 Apr;166(1):313–318. doi: 10.1128/jb.166.1.313-318.1986

GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae.

J F Tschopp, S D Emr, C Field, R Schekman
PMCID: PMC214594  PMID: 3082856

Abstract

The gene encoding the galactose permease of Saccharomyces cerevisiae (GAL2) was cloned. The clone restores galactose permease activity to gal2 yeasts and is regulated by galactose in a manner similar to other GAL gene products (GAL1, -7, and -10). Experiments with temperature-conditional secretory mutants indicated that transport of the GAL2 gene product to the cell surface requires a functional secretory pathway. In addition, gene fusions were constructed between the GAL2 gene and the Escherichia coli lacZ gene. The GAL2-lacZ gene fusions code for galactose-regulated beta-galactosidase activity in yeasts. The beta-galactosidase activity was found to be membrane bound.

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Selected References

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